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single-cells RNA-seq analysis by MAST with unbalanced group size
mast
single-cell
rna-seq
differential analysis
updated 25 days ago by
achoppe
▴ 10 • written 7.3 years ago by
shao
▴ 100
3
votes
3
replies
1.2k
views
Robust way of dealing with low number of samples for Differential Gene Expression
limma
edgeR
DESeq2
MAST
written 6 months ago by
Satoshi
• 0
0
votes
1
reply
1.4k
views
Low logFCs using MAST for scRNA-seq
MAST
updated 8 months ago by
nhaus
▴ 70 • written 3.1 years ago by
learner_MD
• 0
0
votes
1
reply
632
views
Which input data is acceptable for MAST?
SCTransform
MAST
updated 6 months ago by
Andrew_McDavid
▴ 280 • written 6 months ago by
james.watson1
• 0
0
votes
0
replies
381
views
MAST package: Most appropriate way to perform lrTest for effect of factor with multiple levels in pairwise manner?
MAST
9 months ago
Kelly
• 0
0
votes
0
replies
599
views
Linear Mixed Model for Differential Gene Expression in Single-Cell RNAseq with Batch Effects
lmer
MAST
DifferentialExpression
9 months ago
nhaus
▴ 70
0
votes
0
replies
180
views
Confidence interval contains 0 but p<0.05
MAST
4 months ago
Adelyn
• 0
0
votes
0
replies
402
views
Z-score Interpretation across components
MAST
7 months ago
GJ
• 0
0
votes
1
reply
1.6k
views
How to do pairwise multiple comparisons in MAST
MAST
updated 9 months ago by
Kelly
• 0 • written 4.2 years ago by
sysumed3615
• 0
9 results • Page
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Comment: GSEA preranked analysis downstream of DEseq2
by
Ajay
• 0
`logFC * -log10(pval)` seems to be the pi-score proposed in this [paper][1] [1]: https://pmc.ncbi.nlm.nih.gov/articles/PMC3957066/
Comment: error in ChAMP loading file
by
janwilseo
• 0
Any update on this post? I also had an issue with this. [erosion control](https://charlestonhydroseeding.com/)
Answer: CluserProfiler message "No gene can be mapped"
by
James W. MacDonald
68k
Two things. First, the order of your term2gene is backwards (should be term and then gene, like the name), and by default `enricher` adjust…
Comment: Controlling for batch and estrus cycle using DESeq2 in RNA-seq analysis
by
donnycrimson
• 0
One potential workaround might be to adjust for these factors using a combination of design matrices perhaps including batch as a covariate…
Comment: DESeq2 Design controlling for gender
by
donnycrimson
• 0
On a lighter note, while you are tackling such complex data, you might enjoy a quick break playing the [Slope Game](https://slopegame.lol)…
Votes
DiffBind; Error: No sites have activity greater than filter value
remove X and Y chromosome genes in RNA-seq data using DESeq2 pipeline
How to remove X & Y chromosome genes from RNAseq data
Is it advisable to remove X and Y chromosome genes in mouse bulk RNA-seq data at the level of the count matrix?
A: Unbalanced experiment with multiple samples from each patient.
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