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normalisation
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2
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2
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895
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Double normalising when using sva and deseq2
DESeq2
Normalisation
Bulkrnaseq
Svaseq
16 months ago
BioinfGuru
▴ 70
1
vote
2
replies
870
views
Different regions for normalisation
DESeq2
median-of-ratios
normalisation
2.5 years ago
msrch
• 0
3
votes
2
replies
1.5k
views
Sequencing depth before and aftr normalisation with DESEQ2 doesn't look as expected: Problem?
normalisation
DESEQ2
updated 4.2 years ago by
swbarnes2
★ 1.4k • written 4.2 years ago by
xavier
• 0
0
votes
3
replies
4.2k
views
Normalisation - Deseq2 vs StingTie-Ballgown
deseq2
StringTie
RNA-seq
normalisation
updated 5.9 years ago by
Michael Love
43k • written 5.9 years ago by
JindrichK
• 0
0
votes
7
replies
1.8k
views
DESEQ2 for circRNA normalisation
DESEQ2
circRNA
normalisation
updated 5.9 years ago by
Michael Love
43k • written 5.9 years ago by
zoe.ward
• 0
5 results • Page
1 of 1
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Comment: "Watched Tags" are being erased rather than updated
by
shepherl
4.2k
I can reproduce this and have opened an issue on the code base https://github.com/Bioconductor/support.bioconductor.org/issues/112
Comment: "Watched Tags" are being erased rather than updated
by
shepherl
4.2k
we can investigate this issue. thank you for bringing it to our attention
Comment: deseq2 filter the low counts
by
Michael Love
43k
> But within each comparison (contrast), if I trace some DEGs back to the original salmon merged gene counts (or even to normalised counts)…
Comment: deseq2 filter the low counts
by
empty_mt
• 0
Thank you for you suggestion to look at the pre-filtering section in the DESeq2 vignettes - am I missing something? I have 6 treatments, …
Comment: deseq2 filter the low counts
by
Michael Love
43k
Have you seen this part: https://bioconductor.org/packages/release/bioc/vignettes/DESeq2/inst/doc/DESeq2.html#pre-filtering
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Bioconductor 3.22 Released!
Bioconductor 3.22 Released!
Comment: "Watched Tags" are being erased rather than updated
Comment: deseq2 filter the low counts
Answer: DESeq2 interactions
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