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Comment: Ensembl 115 has been released!
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eja.gsssssswwse.r.m
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Keeping up with Ensembls latest release ensures access to cutting-edge genomic information. For those balancing research and leisure, a mob…
Answer: After running samtools, the featureCounts command crashed.
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Kevin Blighe
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Hi Rui Tang,
The "buffer overflow detected" error in featureCounts usually stems from a corrupted or malformed GTF file, often due to tr…
Answer: Problem with the TMB plot
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Kevin Blighe
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Hi,
The linear TMB plot with no clustering suggests your variant calls may lack sample-specific discrimination, likely due to filtering ap…
Answer: gene Set with high AUC score but no cells assigned to cell type
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Kevin Blighe
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4.0k
Hi,
You have correctly identified the issue: AUCell's automatic threshold selection is conservative by design, aiming for specificity to…
Answer: featureCounts -L: high unassigned:mapq fraction
by
Kevin Blighe
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4.0k
Hi Katarzyna,
The discrepancy arises because your samtools stats were restricted to genic regions only, where alignments are typically o…
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