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ruvseq
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4
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14
replies
10k
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Extracting custom post-normalization counts from DESeq2
deseq2
r
ruvseq
normalization
rnaseq
10.5 years ago
nickp60
• 0
5
votes
3
replies
3.2k
views
edgeR: annotation and isoform analysis alternatives
edgeR
ruvseq
rnaseq
isoforms
10.7 years ago
sergio.espeso-gil
• 0
3
votes
9
replies
6.1k
views
DESeq2 results with RUVSeq values
deseq2
ruvseq
sizefacotrs
updated 10.7 years ago by
davide risso
▴ 980 • written 10.7 years ago by
Assa Yeroslaviz
★ 1.5k
3
votes
2
replies
3.2k
views
Normalisation before RUVg and RUV2?
ruvseq
ruv
updated 10.7 years ago by
davide risso
▴ 980 • written 10.7 years ago by
Pauly Lin
▴ 160
1
vote
8
replies
4.5k
views
Intepretation of RUVseq/edgeR logFC
RUVseq
logFC
edgeR
10.7 years ago
Marie
• 0
1
vote
9
replies
3.9k
views
RUV: removing unwanted variation in RNA-seq
RUV
ruvseq
rnaseq
10.7 years ago
sergio.espeso-gil
• 0
0
votes
1
reply
1.6k
views
RUVSeq installation problem
RUVSeq
11.4 years ago • updated 11.3 years ago
davide risso
▴ 980
2
votes
8
replies
3.5k
views
Issue with RUVs
ruvseq
RUVs EDA
11.4 years ago • updated 11.3 years ago
asif.zubair
• 0
108 results • Page
3 of 3
Recent ...
Replies
Answer: lfc shrinkage estimator in DESeq2
by
plumsgorillas
• 0
You are on the right track. In DESeq2, raw log2 fold changes are often noisy and can be misleading, especially for genes with low counts. A…
Answer: Array weights in dpcDE
by
Gordon Smyth
53k
The uncertainty of quantification is fully accounted for in the dpcDE() analysis, including in the computation of sample weights. However,…
Answer: lfc shrinkage estimator in DESeq2
by
Michael Love
43k
Try the vignette which has a lot of information on these type of questions.
Comment: limpa analysis advice
by
Gordon Smyth
53k
The DPC slope is beta1. The estimated slopes for your data look pretty low, lower than I've ever used for a real dataset. Nevertheless, as …
Comment: limpa analysis advice
by
SamGG
▴ 360
Thanks for your feedback and for reassuring me. :-) As you suggested, I tried dpcCN() and set the slope to 0.7 in addition to the standard…
Votes
A: DESeq2: Is it possible to convert read counts to expression values via TPM and r
A: Filtering read counts matrix: how to deal with duplicated gene symbols, differen
Comment: limpa analysis advice
Answer: When to use edgeR or limma
Answer: When to use edgeR or limma
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