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Incorporating RUVg results into ImpulseDE2 model
ImpulseDE2
ImpulseDE
ERCC
RUVSeq
3.9 years ago
Mallory
▴ 10
3
votes
2
replies
3.9k
views
Using the ERCC spike as a normalization factor for a custom algorithm
ERCC
rna-seq
edgeR
tmm normalised values
updated 8.6 years ago by
Aaron Lun
★ 29k • written 8.6 years ago by
what_theheck24
• 0
27
votes
7
replies
17k
views
Usability of ERCC spike-ins in standard RNAseq experiments
RNAseq
deseq2
ercc
updated 8.9 years ago by
Simon Anders
★ 3.8k • written 8.9 years ago by
mbeckste
▴ 30
16
votes
5
replies
8.4k
views
DESeq and Limma+Voom Normalization for Rna-Seq Data Using Ercc Spike-In
limma
voom
deseq
normalization
ercc
updated 9.8 years ago by
Gordon Smyth
53k • written 9.8 years ago by
komal.rathi
▴ 120
8
votes
5
replies
6.0k
views
edgeR and Normalization of RNAseq data using ERCC controls
edger
ercc
10.8 years ago
John
▴ 10
9
votes
4
replies
3.4k
views
News:
Experimental data package 'seqc'
seqc
rsubread
featurecounts
subjunc
ercc
News
10.8 years ago
Wei Shi
★ 3.6k
6 results • Page
1 of 1
Recent ...
Replies
Comment: Heatmap of the Count Matrix
by
ArnulfoByrd
• 0
Perhaps PCA loadings offer a more comprehensive perspective. Now, thinking back, I recall struggling with a similar data reduction dilemma …
Answer: readBam vs read_bam_insert_metrics
by
James W. MacDonald
68k
Depends on what you mean by input. If you mean to ask if you use `readBam` to generate something to use as an argument to `read_bam_insert_…
Answer: How to construct a BSgenome object from a custom genome assembly using BSgenomeF
by
James W. MacDonald
68k
There is an example in the help page for `forgeBSgenomePkgFromTwobitFile`. Have you tried emulating that?
Answer: Guidance on limma for pseudobulk scRNA-seq with multi-factor design
by
Gordon Smyth
53k
What you describe sounds a good approach to me. I would also use sample weights (`sample.weights=TRUE` when running `voomLmFit`) and robust…
Comment: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
Michael Love
43k
I moved your post from an "answer" to a "comment". I don't know about `offst()` function, what is the scale or direction, or whether you a…
Votes
Answer: Limpa Pipeline Order
A: How barcode-plot enrichment is calculated?
A: How barcode-plot enrichment is calculated?
Answer: ROAST compare results with complex design
How to account for solvent controls in DESeq2 when comparing follicular vs luteal phase?
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