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4
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Fold change calculation in Diffbind vs. DESEQ2?
DiffBind
ChIPSeqData
DESeq2
20 minutes ago
marcusn
• 0
0
votes
1
reply
19
views
putting my own expression data in deseq2
DESeq2
updated 2 hours ago by
ATpoint
★ 4.1k • written 3 hours ago by
Dev
• 0
0
votes
0
replies
21
views
Timecourse RNASeq analysis
DESeq2
ImpulseDE2
timecour
timecoursedata
moanin
5 hours ago
Aurora
• 0
0
votes
0
replies
11
views
How do I select a GC file (gc.wig)? (How to determine the inputdata of HMMcopy)
CopyNumberPlots
HMMcopy
CopyNumberVariation
9 hours ago
SSSJec
• 0
0
votes
0
replies
23
views
Padj "NA" with thousands of low count although the raw read counts are quite large
DESeq2
16 hours ago
Agung
• 0
0
votes
0
replies
31
views
Could we detect difference at pathway level but not significant different at gene level?
GSVA
22 hours ago • updated 20 hours ago
Chris
• 0
0
votes
0
replies
60
views
One error problem occurred caused by data type during using GDCprepare to handle BRCA data
GDCRNATools
TCGA
5 days ago
glaciya2018
• 0
0
votes
0
replies
58
views
how to do Differential splicing from SGSeq analysis
DEXSeq
SGSeq
5 days ago • updated 1 day ago
Sara
• 0
0
votes
0
replies
61
views
Exon-exon Junction Analysis for Variable-length Exons
Rsubread
6 days ago
Dario Strbenac
★ 1.5k
0
votes
8
replies
304
views
DESeq2 output used for PCA plot on R studio
DESeq2
RSTUDIO
PCAtools
updated 5 days ago by
swbarnes2
★ 1.3k • written 6 days ago by
Aaliya
• 0
0
votes
2
replies
200
views
featureCounts reports an error:"featureCounts: input-files.c:2890: SAM_pairer_get_next_read_BIN: Assertion `l_name < 256' failed. Aborted (core dumpe…
metaMSdata
7 days ago • updated 5 days ago
shuwangxinze1996
• 0
1
vote
13
replies
350
views
Trying to use enrichGO
clusterProfiler
GenomicDistributionsData
KEGG
8 days ago • updated 5 days ago
fernanda.backsouza
▴ 10
1
vote
3
replies
833
views
How to tell and visualize up/down regulation of pathways from clusterprofiler gsea output
clusterProfiler
RNASeq
updated 6 days ago by
Guangchuang Yu
★ 1.2k • written 14 months ago by
ruiqi.li
▴ 10
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Answer: Version 3.15 S4Vectors packages are missing
by
shepherl
3.8k
It is missing because that package did not have any updates in the release except for the initial version. The archive directory holds prev…
Comment: putting my own expression data in deseq2
by
ATpoint
★ 4.1k
> directly from GEOquery I can tell you that GEOquery retrieves array data not appropriate for DESeq2. For anything else make your questi…
Comment: Assist me in determining whether the analysis process using the limma package ha
by
SSSJec
• 0
Thanks for your reply. I understand the principle of adjusted P-values, but I still want to address that issue. The description of the G…
Comment: Why p value inconsistent when using different number of pathway score?
by
Gordon Smyth
50k
Just a note that fgsea is not classical GSEA. The classical GSEA as described in the Subramanian paper and other papers from the Broad Inst…
Comment: Perform gene set enrichment analysis using fgsea on two conditions that don't ha
by
Chris
• 0
Thank you! The resource I use may mispresent the requirement input of fgsea.
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Nominations for Bioconductor Awards 2024!
Answer: Improving the design matrices vignette in RNAseq123
Answer: Perform gene set enrichment analysis using fgsea on two conditions that don't ha
Answer: Perform gene set enrichment analysis using fgsea on two conditions that don't ha
Comment: DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
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Matthew Ritchie
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