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Showing :
DeSeq2
•
reset
1
vote
3
replies
1.0k
views
DESeq - help with pair wise comparisons
DESeq2
5 months ago
bd2000
▴ 30
1
vote
8
replies
2.9k
views
DESeq: Gene is not flagged as differentially expressed despite count data showing differences
DifferentialExpression
DESeq2
6 months ago • updated 5 months ago
ktz
• 0
0
votes
1
reply
724
views
DESeq2
org.Mmu.eg.db
DESeq2
TimeCourse
DMSOvsdrug
RNASeqData
updated 6 months ago by
ATpoint
★ 4.9k • written 6 months ago by
Cosmic
• 0
4
votes
6
replies
1.5k
views
Surprising behavior for DEXseq dispersion estimate plot?
DESeq2
DEXSeq
updated 6 months ago by
ATpoint
★ 4.9k • written 7 months ago by
Elijah
• 0
1
vote
4
replies
717
views
DESEQ2 looking at main effects while controlling for a continuous variable
DESeq2
6 months ago
Lennie
• 0
0
votes
4
replies
1.1k
views
Guidance Needed: Best Practices for Handling Technical Replicates in RNA-seq Analysis
DESeq2
TechnicalReplicates
RNASeq
written 6 months ago by
questionanswer
• 0
1
vote
2
replies
1.1k
views
Use of org.Rn.eg.db with GRCr8 reference genome
DESeq2
org.Rn.eg.db
6 months ago • updated 4 months ago
Erika
• 0
1
vote
8
replies
2.6k
views
[ChIP-seq] DESeq2 on counts matrix from union set of called peaks
csaw
ChIPseq
DESeq2
DiffBind
6 months ago • updated 5 months ago
Luca
• 0
0
votes
2
replies
680
views
Removing UTR from GTF for gene quantification
edgeR
DESeq2
updated 6 months ago by
Michael Love
43k • written 6 months ago by
rohitsatyam102
▴ 20
0
votes
1
reply
411
views
DESeq use in lipidomic analysis
DESeq2
package
Lipidomics
updated 7 months ago by
Michael Love
43k • written 7 months ago by
泓宇
• 0
0
votes
3
replies
940
views
how many significant figures are there in DESeq2 output
DESeq2
updated 7 months ago by
ATpoint
★ 4.9k • written 7 months ago by
Matthew McCormack
▴ 180
2
votes
5
replies
1.3k
views
Group-specific condition effects with individuals nested: matrix is not full rank
DESeq2
7 months ago
picasa1983
• 0
3
votes
7
replies
2.3k
views
Vertical line at A=0, nothing else, on MA plot
deseq2
updated 7 months ago by
melissa
▴ 10 • written 6.6 years ago by
david.wheeler7
▴ 20
0
votes
1
reply
772
views
How to adjust for Cell Type Differences between groups in Pseudo-Bulk Differential Gene Expression analysis in scRNA-seq?
SingleCellData
DESeq2
pseudobulk
sing
updated 7 months ago by
James W. MacDonald
68k • written 7 months ago by
Sara
• 0
1
vote
1
reply
624
views
Interpreting DESEQ2 interaction term and inclusion of covariate
DESeq2
updated 7 months ago by
swbarnes2
★ 1.4k • written 7 months ago by
Len
▴ 10
1
vote
1
reply
415
views
DEseq2 design for additive treatment
DESeq2
updated 7 months ago by
ATpoint
★ 4.9k • written 7 months ago by
picasa1983
• 0
2
votes
2
replies
780
views
Plot title for PCA in DESeq2
DESeq2
7 months ago
AL
• 0
0
votes
3
replies
871
views
Differential variance analysis - can i use deseq2
DESeq2
DifferentialExpression
RNASeq
RNASeqData
scRNAseq
updated 7 months ago by
Michael Love
43k • written 7 months ago by
Marwan
• 0
1
vote
2
replies
1.3k
views
Weird p-values using DESeq2?
DESeq2
RNASeq
updated 7 months ago by
ATpoint
★ 4.9k • written 7 months ago by
ingrid.h
• 0
0
votes
1
reply
352
views
Comparing Mock vs Infected in WT and Knockout Cells
DESeq2
DifferentialExpression
DifferentialRegulation
RNASeq
GeneSetEnrichment
updated 7 months ago by
Michael Love
43k • written 7 months ago by
Sahil
• 0
0
votes
3
replies
754
views
DESeq2 adujsted p-value cutoff seemingly not working
DESeq2
updated 7 months ago by
980linnet
• 0 • written 8 months ago by
Guilherme
• 0
0
votes
2
replies
874
views
accounting for vs. removing batch effects with DESeq2 in RNAseq analysis
DESeq2
updated 7 months ago by
Blair
• 0 • written 8 months ago by
A
• 0
0
votes
3
replies
585
views
displaying the differential expression with deseq2 over 4X2 samples with the individual changes in gene expression per sample
DESeq2
updated 7 months ago by
Leonard
• 0 • written 8 months ago by
Martijn
• 0
0
votes
3
replies
793
views
How to compare expression levels of genes between scRNAseq and bulkRNAseq?
Seurat
RNASeq
DESeq2
scRNAseq
updated 8 months ago by
jodan
• 0 • written 8 months ago by
Quang NN
▴ 10
0
votes
1
reply
480
views
DEG analysis across multiple timepoints, treatments and tissues
DifferentialExpression
RNASeq
DESeq2
updated 8 months ago by
Michael Love
43k • written 8 months ago by
Sim
• 0
2
votes
2
replies
652
views
Selection of correct coefficients or contrasts in DESeq2
DESeq2
updated 8 months ago by
swbarnes2
★ 1.4k • written 8 months ago by
Nathan
• 0
0
votes
4
replies
1.3k
views
Why select top highly expressed genes based on normalized counts, but then plot VST-transformed values in DESeq2 pheatmap?
RNASeq
DESeq2
8 months ago
Quang NN
▴ 10
0
votes
2
replies
1.2k
views
How to generate circular cnetplot and color nodes and edges in the same plot ?
cnetplot
DESeq2
8 months ago
Blandine
• 0
0
votes
5
replies
643
views
Is there a way to do DEG analysis across two batches sharing one sample only?
DESeq2
DifferentialExpression
RNASeqData
BatchEffect
written 8 months ago by
georgeyu703
• 0
0
votes
4
replies
913
views
How to sort negative log2FC or FC in RNAseq for GSEA?
DESeq2
GSEA
clusterProfiler
8 months ago
Quang NN
▴ 10
2
votes
5
replies
740
views
Differential variability modelling with vst() expression
limma
DESeq2
written 8 months ago by
jtsy6383
• 0
0
votes
3
replies
846
views
DESeq2 analysis with transcript counts
Transcriptomics
RNASeq
DESeq2
written 8 months ago by
sindhuri
• 0
2
votes
9
replies
1.6k
views
Why did PCA plots look exactly the same after adjusting for batch covariate in DESeq2 design?
DESeq2
BatchEffect
8 months ago
Quang NN
▴ 10
1
vote
1
reply
827
views
Considerations behind the choice of RNAseq Differential Expression Analysis Tools
fishpond
DESeq2
rnaseqDTU
updated 8 months ago by
Michael Love
43k • written 8 months ago by
muhammadsohailraza
• 0
0
votes
1
reply
331
views
Want numbers in DESeq to be seen as numerical, not categorical
DESeq2
updated 9 months ago by
swbarnes2
★ 1.4k • written 9 months ago by
natalievillafranca
• 0
0
votes
1
reply
566
views
Please, I desperately need help with DESeq2. Three groups of repeated measures with two timepoints... (plus an additional group)
edgeR
DESeq2
updated 9 months ago by
Michael Love
43k • written 9 months ago by
Jimmy Mak
• 0
2
votes
1
reply
598
views
Is it possible to work with DESeq2 where data is not properly matched for sex and age
DESeq2
RNASeqData
updated 9 months ago by
Michael Love
43k • written 9 months ago by
KR
• 0
0
votes
2
replies
685
views
DESeq error: counts matrix should be numeric, currently it has mode: character
DESeq2
9 months ago
Kucsera
• 0
1
vote
1
reply
536
views
Can DESeq2's design compensate for sequencing experimental design shortcomings?
DESeq2
updated 9 months ago by
Michael Love
43k • written 9 months ago by
txema.heredia
• 0
1
vote
9
replies
1.4k
views
Normalizing batch effects using technical replicates for DGE analysis between two conditions from 2 different runs
limma
DESeq2
edgeR
written 9 months ago by
anna.cot.anna.cot
▴ 30
4
votes
2
replies
937
views
Standardization of rlog transformed counts before PCA
RNASeq
DESeq2
Visualization
updated 9 months ago by
ATpoint
★ 4.9k • written 9 months ago by
bbao
▴ 10
1
vote
5
replies
943
views
Deseq2 Design
RNASeq
DESeq2
updated 9 months ago by
James W. MacDonald
68k • written 9 months ago by
pm_25
• 0
0
votes
5
replies
1.1k
views
p value is 0, padj is 0
DESeq2
9 months ago
Wirayatida
• 0
1
vote
2
replies
1.1k
views
Pre-filtering TPM kallisto for ssGSEA / DESeq2
tximport
DESeq2
GSVA
9 months ago
georgii.vdovin
• 0
0
votes
7
replies
1.2k
views
DESeq2: Gene is DEG in one experiment but not in the other depite similar baseMean, L2FC, ...
DESeq2
updated 9 months ago by
txema.heredia
• 0 • written 9 months ago by
fjrsa
• 0
0
votes
1
reply
514
views
Deseq2 Error in solve.default(qr.R(qrx)) : 'a' (1 x 0) must be square
DESeq2
updated 10 months ago by
Michael Love
43k • written 10 months ago by
bhandary.8590
▴ 20
0
votes
3
replies
709
views
DESeq2 - Comparing Comparisons
DESeq2
updated 9 months ago by
James W. MacDonald
68k • written 10 months ago by
sk
• 0
0
votes
4
replies
859
views
lfcShrink and alpha value
lfcShrink
DESeq2
10 months ago
cropero
• 0
2
votes
1
reply
663
views
DESeq2- merging technical vs biological replicates
DESeq2
RNASeqData
updated 10 months ago by
Michael Love
43k • written 10 months ago by
Nikita
• 0
0
votes
2
replies
462
views
DESeq2 dispersion estimates fitting drastically different based on explanatory variable scaling
dispersionestimates
DESeq2
updated 10 months ago by
Michael Love
43k • written 10 months ago by
Sam
• 0
4,835 results • Page
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Comment: R course in Boston December 19-20
by
eja.gaasa2e.r.m
• 0
The upcoming XLSolutions R course in Boston on December 19 20 is a great opportunity to enhance your data skills. Whether you re a beginner…
Answer: Gatins Set error
by
James W. MacDonald
68k
`GatingSet` isn't a function in `flowCore`. It is however in `flowWorkspace`, which you would need to load first.
Answer: How to model DE for RNA-seq in concordant monozygotic twins?
by
Gordon Smyth
53k
Your question is more about science than it is about software syntax, and I can't give advice about how to conduct a scientific analysis, n…
Comment: incomplete imputation of missing values with LEA impute() function
by
Gordon Smyth
53k
Nadia, please tag your question with the name of the package ("LEA") that you are asking about. "impute" is the name of a different Biocond…
Comment: Sharing a collection of datasets in ExperimentHub
by
Aaron Lun
★ 29k
FYI the **scRNAseq** packages uses a non-ExperimentHub storage platform, see [here](https://github.com/ArtifactDB/gypsum-worker) for more d…
Votes
Comment: incomplete imputation of missing values with LEA impute() function
Answer: Expected memory usage for analyzing large single-cell seq datasets (> 450,000 ce
Answer: ordinary t from limma [was: How would I normally compare swirl?]
Comment: readDNAStringSet with non DNA characters
Comment: Guidance on limma for pseudobulk scRNA-seq with multi-factor design
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