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LIMMA
•
reset
0
votes
2
replies
120
views
Assist me in determining whether the analysis process using the limma package has been executed correctly.
GEOquery
ArrayExpress
limma
DifferentialExpression
23 hours ago • updated 10 hours ago
SSSJec
• 0
0
votes
1
reply
119
views
limma-voom: Calculate the log-fold change in gene expression within a sample
limma
RNASeq
voom
updated 7 days ago by
Gordon Smyth
50k • written 7 days ago by
taur.vil
• 0
14
votes
17
replies
1.0k
views
Why p value inconsistent when using different number of pathway score?
limma
9 days ago • updated just now
Chris
• 0
0
votes
2
replies
181
views
RNAseq:Technical replicates from different batches
technicalReplicates
BatchEffect
limma
RNASeq
10 days ago • updated 8 days ago
catv
• 0
3
votes
1
reply
150
views
Log-cpm values from limma
limma
edgeR
RNASeq
updated 11 days ago by
Gordon Smyth
50k • written 12 days ago by
Shaimaa Gamal
• 0
0
votes
7
replies
277
views
Methylation Analysis experiment design using Limma
DNAMethylation
limma
methylationArrayAnalysis
13 days ago • updated 7 days ago
hortense96
• 0
5
votes
9
replies
885
views
Light difference when using coef vs omit a group when compare 3 groups.
limma
19 days ago • updated 11 days ago
Chris
• 0
1
vote
3
replies
334
views
Why is my gene not significantly differentially expressed?
limmaGUI
edgeR
limma
updated 15 days ago by
Gordon Smyth
50k • written 20 days ago by
Simran
• 0
2
votes
6
replies
371
views
How to make boxplot with pairwise and overall p value from Limma
t.test
boxplot
limma
anova
21 days ago
Chris
• 0
0
votes
1
reply
153
views
Limma design matrix only showing 3/4 treatments?
limma
designmatrix
updated 24 days ago by
Gordon Smyth
50k • written 24 days ago by
avery
• 0
0
votes
4
replies
296
views
Help with design.matrix
limma
24 days ago • updated 23 days ago
andrea.rodriguez-martinez13
• 0
1
vote
4
replies
420
views
Using a change in a continuous explanatory variable to test changes in gene expression with limma
limma
updated 25 days ago by
Gordon Smyth
50k • written 27 days ago by
jari.karppinen
• 0
0
votes
2
replies
178
views
voom(calcNormFactors()) for normalization of RNAseq counts for WGCNA
Voom
Limma
WGCNA
27 days ago • updated 26 days ago
Gimena
• 0
15
votes
22
replies
1.9k
views
The difference when using Limma compare between 3 conditions vs 2 conditions
limma
4 weeks ago • updated 9 days ago
Chris
• 0
5
votes
25
replies
841
views
limma models out of memory issue on Slurm HPC
Regression
limma
STATISITICS
HPC
updated 25 days ago by
Gordon Smyth
50k • written 5 weeks ago by
Jitendra
▴ 10
0
votes
4
replies
584
views
How to correct for known and surrogate variables in DESeq2?
DESeq2
BatchEffect
sva
limma
updated 13 days ago by
Michael Love
41k • written 6 months ago by
Crystal571
• 0
5
votes
9
replies
1.2k
views
Batch correction and repeated measures
RUVSeq
sva
limma
updated 12 days ago by
Gordon Smyth
50k • written 7 months ago by
Jonathan
▴ 10
5
votes
8
replies
8.8k
views
Limma design with continuous and categorical covariates
limma
batch effect
covariates
microarray
updated 7 days ago by
hortense96
• 0 • written 7.7 years ago by
dmcleanuk
▴ 30
0
votes
6
replies
3.1k
views
limma, analysis on subset of data gives completely different results
limma
limma
updated 28 days ago by
Gordon Smyth
50k • written 9.8 years ago by
Arvid Sondén
▴ 40
19 results • Page
1 of 1
Recent ...
Replies
Answer: Timecourse RNASeq analysis
by
Michael Love
41k
There are many choices for time course, and we have an example in our workflow (rnaseqGene). Otherwise, I recommend to consult a local …
Answer: Padj "NA" with thousands of low count although the raw read counts are quite lar
by
Michael Love
41k
You can either turn off independent filtering in `results` or use `ihw` see the vignette.
Comment: Why p value inconsistent when using different number of pathway score?
by
Chris
• 0
Thanks Gordon for a detail guide! Seem you track time of everything.
Answer: Version 3.15 S4Vectors packages are missing
by
shepherl
3.8k
It is missing because that package did not have any updates in the release except for the initial version. The archive directory holds prev…
Answer: putting my own expression data in deseq2
by
ATpoint
★ 4.1k
> directly from GEOquery I can tell you that GEOquery retrieves array data not appropriate for DESeq2. For anything else make your questi…
Votes
Comment: Why p value inconsistent when using different number of pathway score?
Nominations for Bioconductor Awards 2024!
Answer: Improving the design matrices vignette in RNAseq123
Answer: Perform gene set enrichment analysis using fgsea on two conditions that don't ha
Answer: Perform gene set enrichment analysis using fgsea on two conditions that don't ha
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Chris
• 0
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Matthew Ritchie
▴ 1000
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alserg
▴ 260
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bioinf
▴ 10
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Gordon Smyth
50k
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