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Answer: Consistent Decimals In Data After Antibody Array Background Correction Using Lim
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Gordon Smyth
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`nec` computes one set of negative control parameters (mu, sigma, alpha) for each sample.
Comment: DESeq Input from Salmon + tximport/tximeta
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LuciaNhu
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Thank you to the author for the quick reply. If I use `gene_counts_length_scaled.tsv` as a metric that has already normalized for library s…
Answer: DESeq Input from Salmon + tximport/tximeta
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Michael Love
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Given nf-core's pipeline here, you can use rounded counts from `gene_counts_length_scaled.tsv`.
Comment: Question about using LRT test for time course experiments with DESeq2
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James W. MacDonald
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You don't want to add a logFC on top like that, as it invalidates the p-value.
Maybe I wasn't clear before. The LRT gives you all the gene…
Comment: Question about using LRT test for time course experiments with DESeq2
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Denise
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Hi James,
Thank you very much for your very detailed explanation!
I guess my question is simpler than that, I just want to union, across…
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