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10X
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emptyDrops new default alpha = Inf giving far fewer non-empty droplets than under old default of alpha = NULL
scrn
10x
scRNAseq
emptyDrops
DropletUtils
4 months ago
Peter Hickey
▴ 760
1
vote
1
reply
1.7k
views
Origin of the labels for the SingleCellMultiModal dataset
labels
PBMC
SingleCellMultiModal
10X
updated 8 months ago by
ATpoint
★ 5.0k • written 8 months ago by
javier.ruizramirez
• 0
0
votes
2
replies
1.1k
views
Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
21 months ago
rocanja
▴ 60
0
votes
1
reply
1.5k
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 2.6 years ago by
Peter Hickey
▴ 760 • written 2.6 years ago by
sandra.garces.19
• 0
0
votes
2
replies
1.2k
views
Compare and filter three BAM files
RNAseq
10X
2.7 years ago
wt215
• 0
4
votes
5
replies
4.2k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
3.4 years ago • updated 3.3 years ago
Peter Hickey
▴ 760
1
vote
1
reply
1.6k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 4.9 years ago by
Aaron Lun
★ 29k • written 4.9 years ago by
Peter Hickey
▴ 760
5
votes
3
replies
2.2k
views
Latent factors for differential expression
deseq2
zinbwave
10X
scRNA-seq
Differential expression
updated 5.4 years ago by
Michael Love
43k • written 5.4 years ago by
vincent.croset
▴ 20
4
votes
2
replies
3.8k
views
When to combine samples in the pre-processing of 10x scRNA-seq data?
10x
scrna-seq
updated 6.6 years ago by
Aaron Lun
★ 29k • written 6.6 years ago by
jma1991
▴ 70
7
votes
12
replies
9.4k
views
How to identify real cells in 10X RNA-seq ?
single-cell
10X
Cell calling
DropletUtils
cellranger
updated 6.8 years ago by
Aaron Lun
★ 29k • written 6.8 years ago by
xingxd16
▴ 20
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Answer: NUPOP usage
by
shepherl
4.2k
You should read the documentation in the package especially the package vignette https://bioconductor.org/packages/release/bioc/html/NuPoP.…
Comment: How can I correctly use phyloseq with Docker?
by
fnfgame
• 0
In a rocker image, this error usually means the Bioconductor install happened as root but the runtime library paths used by the shiny user …
Comment: DECIPHER DesignProbes unable to design FISH-probes
by
Erik Wright
▴ 150
Are the sequences aligned (i.e., the same width) before running `TileSeqs()`?
Comment: BiocParallel (and DESeq2) - wrong args for environment subassignment
by
Michael Love
43k
BTW, I tested with no issue on MacOS ARM on our previous github issue.
Answer: DESeq(dds, parallel=TRUE) wrong args for environment subassignment
by
Michael Love
43k
The DESeq2 error makes sense, as we've talked about, given that the simple `bplapply` isn't working in your setup. Maybe give the traceb…
Votes
C: DESeq2 Following RSEM
Upcoming Ensembl API and data access changes - new blog post available
Comment: Highly similar RNA-seq samples in PCA - pooling or technical duplication?
Answer: Large logFC but somewhat high FDR
Comment: upstream git branches of bioc-release
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