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10X
•
reset
0
votes
2
replies
628
views
Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
9 months ago
rocanja
▴ 60
0
votes
1
reply
979
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 19 months ago by
Peter Hickey
▴ 740 • written 19 months ago by
sandra.garces.19
• 0
0
votes
2
replies
923
views
Compare and filter three BAM files
RNAseq
10X
21 months ago
wt215
• 0
4
votes
5
replies
3.2k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
2.4 years ago • updated 2.3 years ago
Peter Hickey
▴ 740
1
vote
1
reply
1.2k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 3.9 years ago by
Aaron Lun
★ 28k • written 3.9 years ago by
Peter Hickey
▴ 740
5
votes
3
replies
1.6k
views
Latent factors for differential expression
deseq2
zinbwave
10X
scRNA-seq
Differential expression
updated 4.4 years ago by
Michael Love
43k • written 4.4 years ago by
vincent.croset
▴ 20
4
votes
2
replies
3.0k
views
When to combine samples in the pre-processing of 10x scRNA-seq data?
10x
scrna-seq
updated 5.6 years ago by
Aaron Lun
★ 28k • written 5.6 years ago by
jma1991
▴ 70
7
votes
12
replies
7.9k
views
How to identify real cells in 10X RNA-seq ?
single-cell
10X
Cell calling
DropletUtils
cellranger
updated 5.8 years ago by
Aaron Lun
★ 28k • written 5.8 years ago by
xingxd16
▴ 20
8 results • Page
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Comment: Cross-validation with multiple control subgroups in limma
by
Ali Barry
▴ 40
Thanks so much for taking the time here, the insights are much appreciated. I suspected I'd have to ad hoc compare results if I did repeat …
Answer: Cross-validation with multiple control subgroups in limma
by
Gordon Smyth
52k
limma doesn't do cross-validation or resampling. Like most classical linear modeling procedures in statistics, limma uses a statistical mod…
Comment: Issues with seqlevelsStyle when making custom txdb objects for genomes/annotatio
by
Robert Castelo
★ 3.4k
Hi Hervé, thank you very much for your input into how to properly build and manage TxDb objects. gDNAx has two main inputs, one or more BAM…
Comment: Biostrings: Error writing long reads (>200 kbps) with writeQualityScaledXStringS
by
Aidan
▴ 60
Quick update: this is fixed in a branch awaiting PR to Biostrings (https://github.com/Bioconductor/Biostrings/pull/122). Should be in `deve…
Answer: Can DESeq2's design compensate for sequencing experimental design shortcomings?
by
Michael Love
43k
For statistical analysis plans, I recommend working with a local statistician or someone familiar with linear models in R. I have to reserv…
Votes
C: edgeR and lack of counts ID on CPM matrix
A: DESeq2 Following RSEM
A: importing RSEM data into DESeq2
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