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10X
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emptyDrops new default alpha = Inf giving far fewer non-empty droplets than under old default of alpha = NULL
scrn
10x
scRNAseq
emptyDrops
DropletUtils
12 weeks ago • updated 11 weeks ago
Peter Hickey
▴ 760
1
vote
1
reply
1.6k
views
Origin of the labels for the SingleCellMultiModal dataset
labels
PBMC
SingleCellMultiModal
10X
updated 6 months ago by
ATpoint
★ 5.0k • written 6 months ago by
javier.ruizramirez
• 0
0
votes
2
replies
1.0k
views
Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
19 months ago
rocanja
▴ 60
0
votes
1
reply
1.5k
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 2.5 years ago by
Peter Hickey
▴ 760 • written 2.5 years ago by
sandra.garces.19
• 0
0
votes
2
replies
1.2k
views
Compare and filter three BAM files
RNAseq
10X
2.6 years ago
wt215
• 0
4
votes
5
replies
4.1k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
3.3 years ago • updated 3.2 years ago
Peter Hickey
▴ 760
1
vote
1
reply
1.5k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 4.8 years ago by
Aaron Lun
★ 29k • written 4.8 years ago by
Peter Hickey
▴ 760
5
votes
3
replies
2.1k
views
Latent factors for differential expression
deseq2
zinbwave
10X
scRNA-seq
Differential expression
updated 5.3 years ago by
Michael Love
43k • written 5.3 years ago by
vincent.croset
▴ 20
4
votes
2
replies
3.7k
views
When to combine samples in the pre-processing of 10x scRNA-seq data?
10x
scrna-seq
updated 6.5 years ago by
Aaron Lun
★ 29k • written 6.5 years ago by
jma1991
▴ 70
7
votes
12
replies
9.3k
views
How to identify real cells in 10X RNA-seq ?
single-cell
10X
Cell calling
DropletUtils
cellranger
updated 6.7 years ago by
Aaron Lun
★ 29k • written 6.7 years ago by
xingxd16
▴ 20
10 results • Page
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Comment: "Watched Tags" are being erased rather than updated
by
shepherl
4.2k
I can reproduce this and have opened an issue on the code base https://github.com/Bioconductor/support.bioconductor.org/issues/112
Comment: "Watched Tags" are being erased rather than updated
by
shepherl
4.2k
we can investigate this issue. thank you for bringing it to our attention
Comment: deseq2 filter the low counts
by
Michael Love
43k
> But within each comparison (contrast), if I trace some DEGs back to the original salmon merged gene counts (or even to normalised counts)…
Comment: deseq2 filter the low counts
by
empty_mt
• 0
Thank you for you suggestion to look at the pre-filtering section in the DESeq2 vignettes - am I missing something? I have 6 treatments, …
Comment: deseq2 filter the low counts
by
Michael Love
43k
Have you seen this part: https://bioconductor.org/packages/release/bioc/vignettes/DESeq2/inst/doc/DESeq2.html#pre-filtering
Votes
Bioconductor 3.22 Released!
Bioconductor 3.22 Released!
Comment: "Watched Tags" are being erased rather than updated
Comment: deseq2 filter the low counts
Answer: DESeq2 interactions
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