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10X
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11
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emptyDrops new default alpha = Inf giving far fewer non-empty droplets than under old default of alpha = NULL
scrn
10x
scRNAseq
emptyDrops
DropletUtils
5 months ago
Peter Hickey
▴ 760
1
vote
1
reply
1.7k
views
Origin of the labels for the SingleCellMultiModal dataset
labels
PBMC
SingleCellMultiModal
10X
updated 9 months ago by
ATpoint
★ 5.0k • written 9 months ago by
javier.ruizramirez
• 0
0
votes
2
replies
1.2k
views
Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
22 months ago
rocanja
▴ 60
0
votes
1
reply
1.6k
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 2.7 years ago by
Peter Hickey
▴ 760 • written 2.7 years ago by
sandra.garces.19
• 0
0
votes
2
replies
1.3k
views
Compare and filter three BAM files
RNAseq
10X
2.8 years ago
wt215
• 0
4
votes
5
replies
4.8k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
3.5 years ago • updated 3.4 years ago
Peter Hickey
▴ 760
1
vote
1
reply
1.7k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 5.0 years ago by
Aaron Lun
★ 29k • written 5.0 years ago by
Peter Hickey
▴ 760
5
votes
3
replies
2.3k
views
Latent factors for differential expression
deseq2
zinbwave
10X
scRNA-seq
Differential expression
updated 5.5 years ago by
Michael Love
43k • written 5.5 years ago by
vincent.croset
▴ 20
4
votes
2
replies
3.9k
views
When to combine samples in the pre-processing of 10x scRNA-seq data?
10x
scrna-seq
updated 6.7 years ago by
Aaron Lun
★ 29k • written 6.7 years ago by
jma1991
▴ 70
7
votes
12
replies
10k
views
How to identify real cells in 10X RNA-seq ?
single-cell
10X
Cell calling
DropletUtils
cellranger
updated 6.9 years ago by
Aaron Lun
★ 29k • written 6.9 years ago by
xingxd16
▴ 20
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Comment: Bile acid analysis with limpa
by
ATpoint
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Wilcox/Mann-Whitney-U cannot work because it is bound to sample size even when all of group X are lower than all of group Y. At 4 vs 3 the …
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Jeremy
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Comment: Making a Full Rank Model for Allele-Specific Expression with DESeq2
by
Gordon Smyth
53k
The design matrix that I described above is completely general. I imagine that it would be possible to use the same approach to generate fu…
Comment: Making a Full Rank Model for Allele-Specific Expression with DESeq2
by
chp265
• 0
Cool, glad to hear I wasn't missing some functionality in DESeq2. I don't know that much about the differences between DESeq2 vs edgeR; my …
Comment: Making a Full Rank Model for Allele-Specific Expression with DESeq2
by
Michael Love
43k
Yes, so for one thing, note that Gordon has provided code for doing what you are asking in edgeR, which you can go ahead and use. You aske…
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Answer: When to use edgeR or limma
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