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10X
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Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
12 months ago
rocanja
▴ 60
0
votes
1
reply
1.1k
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 22 months ago by
Peter Hickey
▴ 750 • written 22 months ago by
sandra.garces.19
• 0
0
votes
2
replies
994
views
Compare and filter three BAM files
RNAseq
10X
2.0 years ago • updated 24 months ago
wt215
• 0
4
votes
5
replies
3.6k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
2.6 years ago
Peter Hickey
▴ 750
1
vote
1
reply
1.3k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 4.2 years ago by
Aaron Lun
★ 28k • written 4.2 years ago by
Peter Hickey
▴ 750
5
votes
3
replies
1.8k
views
Latent factors for differential expression
deseq2
zinbwave
10X
scRNA-seq
Differential expression
updated 4.7 years ago by
Michael Love
43k • written 4.7 years ago by
vincent.croset
▴ 20
4
votes
2
replies
3.3k
views
When to combine samples in the pre-processing of 10x scRNA-seq data?
10x
scrna-seq
updated 5.9 years ago by
Aaron Lun
★ 28k • written 5.9 years ago by
jma1991
▴ 70
7
votes
12
replies
8.4k
views
How to identify real cells in 10X RNA-seq ?
single-cell
10X
Cell calling
DropletUtils
cellranger
updated 6.0 years ago by
Aaron Lun
★ 28k • written 6.0 years ago by
xingxd16
▴ 20
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Comment: DiffBind custom normalization factor
by
Abbie
• 0
Hello! Thanks so much for adding dba.normalize and all the documentation in the manual! I'm hoping I can ask a follow-up question here. I…
Answer: CpGs with genomic positions greater than the chromosome size in EPICv2?
by
James W. MacDonald
68k
All of the annotation packages provided by Bioconductor are simply repackaging of existing data from one or more sources. In this case, the…
Comment: Using TPM from tximport of Salmon output for singscore
by
Kent
• 0
Thank you for the suggestions Michael. Would you say that after filtering out the low count genes, `rlog(counts, blind = T)` is doing the s…
Comment: Using TPM from tximport of Salmon output for singscore
by
Michael Love
43k
For clustering generally we recommend `vst()` see the workflow for example. For working with individual samples, I can see how a scaling/t…
Comment: DESeq2 model - How to test case/control time course model when taking account wi
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43k
I'd recommend working with a local statistician or someone familiar with linear models in R. I have to reserve my time on the support site …
Votes
clusterProfiler-GO enrichment Error
A: Machine learning in R book
Data preparation and Machine learning on time course RNAseq data
Data preparation and Machine learning on time course RNAseq data
Answer: Use of org.Rn.eg.db with GRCr8 reference genome
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