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10X
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reset
0
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11
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emptyDrops new default alpha = Inf giving far fewer non-empty droplets than under old default of alpha = NULL
scrn
10x
scRNAseq
emptyDrops
DropletUtils
9 weeks ago • updated 8 weeks ago
Peter Hickey
▴ 760
1
vote
1
reply
1.6k
views
Origin of the labels for the SingleCellMultiModal dataset
labels
PBMC
SingleCellMultiModal
10X
updated 6 months ago by
ATpoint
★ 5.0k • written 6 months ago by
javier.ruizramirez
• 0
0
votes
2
replies
994
views
Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
19 months ago
rocanja
▴ 60
0
votes
1
reply
1.4k
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 2.4 years ago by
Peter Hickey
▴ 760 • written 2.4 years ago by
sandra.garces.19
• 0
0
votes
2
replies
1.2k
views
Compare and filter three BAM files
RNAseq
10X
2.6 years ago
wt215
• 0
4
votes
5
replies
4.1k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
3.2 years ago
Peter Hickey
▴ 760
1
vote
1
reply
1.5k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 4.7 years ago by
Aaron Lun
★ 29k • written 4.7 years ago by
Peter Hickey
▴ 760
5
votes
3
replies
2.1k
views
Latent factors for differential expression
deseq2
zinbwave
10X
scRNA-seq
Differential expression
updated 5.2 years ago by
Michael Love
43k • written 5.2 years ago by
vincent.croset
▴ 20
4
votes
2
replies
3.7k
views
When to combine samples in the pre-processing of 10x scRNA-seq data?
10x
scrna-seq
updated 6.5 years ago by
Aaron Lun
★ 29k • written 6.5 years ago by
jma1991
▴ 70
7
votes
12
replies
9.2k
views
How to identify real cells in 10X RNA-seq ?
single-cell
10X
Cell calling
DropletUtils
cellranger
updated 6.6 years ago by
Aaron Lun
★ 29k • written 6.6 years ago by
xingxd16
▴ 20
10 results • Page
1 of 1
Recent ...
Replies
Comment: Help with defining groups
by
Phinney, Brett
▴ 10
I got it working , thank you!!!
Comment: Check removeBatchEffect effectiveness
by
massimo
• 0
I would kindly ask for additional support. When not including the design_timepoint argument in removeBatchEffect and thus keeping the inter…
Comment: Error Loading Experiment from SimBenchData Package
by
ksankaran
• 0
Aha, that was it! The object was class Seurat, but I didn't have it installed. After installing, everything works as expected.
Comment: Streamlining the computing time for MiloDE p-value correction in large dataset?
by
ATpoint
★ 5.0k
Is a method that is so computationally expensive really beneficial and necessary? You seem to have biological replication, don't you, so ps…
Answer: NA values for DESeq2 p-values and adjusted p-values using large sample sizes
by
ATpoint
★ 5.0k
Outliers without replacement, independent filtering or all-zero counts for that gene. That's the three options that trigger NAs https://bio…
Votes
Comment: Check removeBatchEffect effectiveness
Gene expression associated with continuous (quantitative) traits (Limma/edgeR/correlation)
Answer: Error Loading Experiment from SimBenchData Package
Comment: incomplete imputation of missing values with LEA impute() function
Answer: Expected memory usage for analyzing large single-cell seq datasets (> 450,000 ce
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