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10x
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0
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Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
12 months ago
rocanja
▴ 60
0
votes
1
reply
1.1k
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 22 months ago by
Peter Hickey
▴ 750 • written 23 months ago by
sandra.garces.19
• 0
0
votes
2
replies
998
views
Compare and filter three BAM files
RNAseq
10X
2.0 years ago
wt215
• 0
4
votes
5
replies
3.6k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
2.6 years ago
Peter Hickey
▴ 750
1
vote
1
reply
1.3k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 4.2 years ago by
Aaron Lun
★ 28k • written 4.2 years ago by
Peter Hickey
▴ 750
5
votes
3
replies
1.8k
views
Latent factors for differential expression
deseq2
zinbwave
10X
scRNA-seq
Differential expression
updated 4.7 years ago by
Michael Love
43k • written 4.7 years ago by
vincent.croset
▴ 20
4
votes
2
replies
3.3k
views
When to combine samples in the pre-processing of 10x scRNA-seq data?
10x
scrna-seq
updated 5.9 years ago by
Aaron Lun
★ 28k • written 5.9 years ago by
jma1991
▴ 70
7
votes
12
replies
8.5k
views
How to identify real cells in 10X RNA-seq ?
single-cell
10X
Cell calling
DropletUtils
cellranger
updated 6.1 years ago by
Aaron Lun
★ 28k • written 6.1 years ago by
xingxd16
▴ 20
8 results • Page
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Comment: Annotation package for Manduca sexta
by
GA
▴ 10
Thank you!
Comment: Improving performance of edgeR and limma while adjusting for multiple confounder
by
ATpoint
★ 4.8k
If 2 minutes is inacceptable to you then you can still fall back to limma-trend which should give a dramatic speedup and finish in seconds.…
Comment: getBM from biomaRt inconsistencies found with .gtf files across multiple species
by
Mike Smith
★ 6.6k
Great, thanks for the update from Ensembl. So the most relevant part of 'Location: Scaffold HG1277_PATCH: 137,021-141,806 forward strand' …
Answer: Improving performance of edgeR and limma while adjusting for multiple confounder
by
Gordon Smyth
52k
We recommend that you use voomLmFit() instead of voom+lmFit. We are pretty proud of the speed of glmQLFit and voom, and we hadn't expected…
Answer: Merging EPICV1 and V2 betas
by
James W. MacDonald
68k
It appears that [SeSAMe][1] has functions meant to do that sort of thing. [1]: https://bioconductor.org/packages/release/bioc/vignet…
Votes
Looking for equivalent EPIC chip v1 vs. v2 annotation variables
Properly constructing a hypergeometric test
A: Is logFC a log2 fold change or some other base in scran::findMarkers
Comment: Error in retrieving clinical data
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