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11
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emptyDrops new default alpha = Inf giving far fewer non-empty droplets than under old default of alpha = NULL
scrn
10x
scRNAseq
emptyDrops
DropletUtils
6 months ago
Peter Hickey
▴ 760
1
vote
1
reply
1.7k
views
Origin of the labels for the SingleCellMultiModal dataset
labels
PBMC
SingleCellMultiModal
10X
updated 10 months ago by
ATpoint
★ 5.0k • written 10 months ago by
javier.ruizramirez
• 0
0
votes
2
replies
1.3k
views
Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
23 months ago
rocanja
▴ 60
0
votes
1
reply
1.6k
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 2.8 years ago by
Peter Hickey
▴ 760 • written 2.8 years ago by
sandra.garces.19
• 0
0
votes
2
replies
1.3k
views
Compare and filter three BAM files
RNAseq
10X
2.9 years ago
wt215
• 0
4
votes
5
replies
5.1k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
3.5 years ago
Peter Hickey
▴ 760
1
vote
1
reply
1.7k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 5.1 years ago by
Aaron Lun
★ 29k • written 5.1 years ago by
Peter Hickey
▴ 760
5
votes
3
replies
2.3k
views
Latent factors for differential expression
deseq2
zinbwave
10X
scRNA-seq
Differential expression
updated 5.6 years ago by
Michael Love
43k • written 5.6 years ago by
vincent.croset
▴ 20
4
votes
2
replies
4.0k
views
When to combine samples in the pre-processing of 10x scRNA-seq data?
10x
scrna-seq
updated 6.8 years ago by
Aaron Lun
★ 29k • written 6.8 years ago by
jma1991
▴ 70
7
votes
12
replies
10k
views
How to identify real cells in 10X RNA-seq ?
single-cell
10X
Cell calling
DropletUtils
cellranger
updated 6.9 years ago by
Aaron Lun
★ 29k • written 6.9 years ago by
xingxd16
▴ 20
10 results • Page
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Answer: Should I use beta1 from limpa::dpc() or limpa::dpcCN(), if they differ?
by
Gordon Smyth
53k
I tend to use dpcCN(), because it more robust than dpc() to experiments with lots of samples and big differences between samples. You coul…
Answer: Which preprocessing steps are needed before running limpa?
by
Gordon Smyth
53k
Please see the example limpa analyses given at <http://github.com/smythlab/limpa>, especially the kidney cancer case study. limpa does not…
Comment: Why does IDTaxa trainingSet for SILVA contain "_2" suffixes in the taxonomy name
by
Korneel
• 0
Thanks for the quick reply! If I understand correctly, I think if I keep track of the full lineage, I shouldn't have any risk of collapsin…
Comment: Pathway abundance data from Curatedmetagenomics
by
yesquokkan
• 0
Hello, I was also wondering about the same issue. Did you figure it out?
Comment: [DESeq2] Changing dispersion estimates between runs of DESeq2
by
colinmccornack
• 0
FWIW: This seems to be due to architectural differences between two compute nodes I was running on, specifically with the DESeq2:::fitNbino…
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Answer: Which preprocessing steps are needed before running limpa?
Answer: Should I use beta1 from limpa::dpc() or limpa::dpcCN(), if they differ?
Answer: Which preprocessing steps are needed before running limpa?
Answer: Which preprocessing steps are needed before running limpa?
Answer: Should I use beta1 from limpa::dpc() or limpa::dpcCN(), if they differ?
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