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Comment: Small RNA-seq Analysis DESEQ2 : Unexpected Significance
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ATpoint
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"Raw" counts, so you're plotting un-normalized data, hence plots have no meaning in terms of groupwise comparisons. Use the `normTransform`…
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I have found a workaround : running dba.blacklist **after** running dba.count (while running dba.count with minOverlap=1).
```
dbObj_co…
Comment: Small RNA-seq Analysis DESEQ2 : Unexpected Significance
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By applying the log2 transformation to the raw counts of small RNAS AND after adding 1 to each count, the resulting values often approximat…
Comment: Small RNA-seq Analysis DESEQ2 : Unexpected Significance
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and sometimes in other count plots we observe a difference between the counts of the 2 conditions (ON/OFF) where it should be significant b…
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What is `Log2 Counts + 1`? Please show code for the plots. And please remove the comments and move everything to the toplevel question usin…
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