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Answer: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
yongjie.wang
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Thanks for your reply.
" normFactors <- exp(-1 * offst(set2))
normFactors <- normFactors / exp(rowMeans(log(normFactors)))
normalizat…
Answer: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
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I'm not familiar with this usage, or code here
```
normFactors <- exp(-1 * offst(set2))
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The fitted values are on the log2CPM scale. I would have told you if they were not!
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