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rnaseqpower
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Are unequal sample sizes in differential gene expression (DGE) analysis a problem for edgeR, DESeq2, and NOISeq? Could you give me some advice on how…
RNA
RNASeqPower
DEGseq
written 14 months ago by
Jonathan
• 0
1
vote
3
replies
2.1k
views
featureCounts fails to load annotation file
featurecounts
rnas
RNASeqPower
15 months ago
yasmin.hilliam
▴ 10
2
votes
2
replies
806
views
Batch adjustment for cohort based RNA seq data
RNASeqPower
removeBatchEffect
cpm
Batcheffect
16 months ago
sukeshinik5
▴ 20
1
vote
2
replies
1.3k
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RNASeq time course analysis, missing time points for control
RNASeqData
RnaSeqSampleSize
RNASeq
RNASeqPower
2.1 years ago
drewhaydensteele
• 0
0
votes
1
reply
1.1k
views
Comparing RNASeq data from multiple studies
RNASeqData
RnaSeqSampleSize
RNASeqPower
RNASeq
updated 2.3 years ago by
Basti
▴ 780 • written 2.3 years ago by
Luke
• 0
1
vote
3
replies
1.7k
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Is it okay to increase filtering to get more significant Adjusted P Values for DEGs found using Limma?
RNASeqPower
edgeR
DifferentialExpression
limma
RNASeq
updated 2.7 years ago by
Gordon Smyth
53k • written 2.7 years ago by
Sara
• 0
0
votes
0
replies
869
views
Typo in citation for RNASeqPower
RNASeqPower
citation
3.6 years ago
stephenjjohnson
• 0
0
votes
3
replies
3.3k
views
How to calculate coefficient of variation within group?
edgeR
limma
RNASeqPower
updated 3.9 years ago by
Gordon Smyth
53k • written 4.9 years ago by
Ahdee
▴ 60
0
votes
5
replies
3.1k
views
getting sample size for RNAseq experiment
rnaseqpower
sspa
sample size
sequencing
complex-design
updated 9.9 years ago by
m.van_iterson
▴ 20 • written 9.9 years ago by
roser.navarro
• 0
0
votes
1
reply
1.5k
views
Depth or # of reads for RNASeqPower
rnaseqpower
11.2 years ago
drnknmstrr
• 0
0
votes
0
replies
1.4k
views
Power Calculation of multiple factors design of RNA-sequencing study
edgeR
DESeq
RNASeqPower
edgeR
DESeq
RNASeqPower
11.9 years ago
Guest User
★ 13k
11 results • Page
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Comment: DESeq2 - how many surrogate variables from svaseq, use of lfcshrink
by
ja569116
• 0
Hi @atpoint Thanks for the information. Could you please expand on when to use adjusted p-values vs nominal p-values. My understanding was …
Answer: limpa analysis advice
by
Gordon Smyth
53k
It is actually very hard to estimate the DPC from observed data, because the curve is function of unobserved intensities rather than a func…
Answer: When to use edgeR or limma
by
Gordon Smyth
53k
The short answer is that we generally prefer edgeR for applications with lots of small counts and limma for complex designs with random eff…
Comment: limpa-blank normalization and Spectronaut's PTM stoichiometry
by
Gordon Smyth
53k
I updated the above answer today.
Answer: When to use edgeR or limma
by
James W. MacDonald
68k
I would use edgeR's quasi-likelihood model for any analysis with a smaller number of observations (like 3 vs 3 or similar), but if you have…
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Answer: When to use edgeR or limma
Answer: When to use edgeR or limma
Answer: When to use edgeR or limma
Answer: When to use edgeR or limma
A: deseq2 - paired samples in 2 sequencing types with very different library sizes
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