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DESeq2
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How to compare expression levels of genes between scRNAseq and bulkRNAseq?
Seurat
RNASeq
DESeq2
scRNAseq
4 hours ago
Quang NN
▴ 10
0
votes
0
replies
10
views
Why select top highly expressed genes based on normalized counts, but then plot VST-transformed values in DESeq2 pheatmap?
RNASeq
DESeq2
4 hours ago
Quang NN
▴ 10
0
votes
1
reply
139
views
Selection of correct coefficients or contrasts in DESeq2
DESeq2
updated 1 day ago by
James W. MacDonald
67k • written 1 day ago by
Nathan
• 0
0
votes
2
replies
213
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How to generate circular cnetplot and color nodes and edges in the same plot ?
cnetplot
DESeq2
3 days ago • updated 1 day ago
Blandine
• 0
0
votes
4
replies
179
views
Is there a way to do DEG analysis across two batches sharing one sample only?
DESeq2
DifferentialExpression
RNASeqData
BatchEffect
4 days ago
georgeyu703
• 0
0
votes
4
replies
358
views
How to sort negative log2FC or FC in RNAseq for GSEA?
DESeq2
GSEA
clusterProfiler
5 days ago • updated 3 days ago
Quang NN
▴ 10
2
votes
9
replies
747
views
Why did PCA plots look exactly the same after adjusting for batch covariate in DESeq2 design?
DESeq2
BatchEffect
17 days ago • updated 5 days ago
Quang NN
▴ 10
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Answer: Postdoc/RA position: Software Engineer For R and High-Performance Computing
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Answer: Issues with seqlevelsStyle when making custom txdb objects for genomes/annotatio
by
Robert Castelo
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Following the discussion of this post, the package [gDNAx](https://bioconductor.org/packages/gDNAx) has been fixed in devel and release 1.4…
Answer: Selection of correct coefficients or contrasts in DESeq2
by
James W. MacDonald
67k
Using R's model formulation for three-way interactions is probably not the way to go here. Or at least I wouldn't do it that way. An easier…
Comment: How to generate circular cnetplot and color nodes and edges in the same plot ?
by
Blandine
• 0
Thank you so much ! It's working !
Comment: how should I apply "cpg.annotate" to TCGA methylation data in hg38 for HM450K?
by
James W. MacDonald
67k
Yes. If you have the Illumina IDs, then all the annotation data that `cpg.annotate` uses will be based on hg19. It doesn't matter if TCGA l…
Votes
A: DESeq2 interaction term
Comment: Recount3 data correspond to TCGA data
Answer: DESeq2- merging technical vs biological replicates
Answer: SingleCellExperiment convenience function for metadata+expression?
How to correct for tumor purity using DESeq2 to analyze TCGA tumor sample expression.
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