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DEseq2
•
reset
4
votes
2
replies
1.2k
views
Standardization of rlog transformed counts before PCA
RNASeq
DESeq2
Visualization
updated 13 months ago by
ATpoint
★ 5.0k • written 13 months ago by
bbao
▴ 10
1
vote
5
replies
1.2k
views
Deseq2 Design
RNASeq
DESeq2
updated 13 months ago by
James W. MacDonald
68k • written 13 months ago by
pm_25
• 0
0
votes
5
replies
1.3k
views
p value is 0, padj is 0
DESeq2
13 months ago
Wirayatida
• 0
1
vote
2
replies
1.3k
views
Pre-filtering TPM kallisto for ssGSEA / DESeq2
tximport
DESeq2
GSVA
13 months ago
georgii.vdovin
• 0
0
votes
7
replies
1.4k
views
DESeq2: Gene is DEG in one experiment but not in the other depite similar baseMean, L2FC, ...
DESeq2
updated 13 months ago by
txema.heredia
• 0 • written 13 months ago by
fjrsa
• 0
0
votes
1
reply
595
views
Deseq2 Error in solve.default(qr.R(qrx)) : 'a' (1 x 0) must be square
DESeq2
updated 13 months ago by
Michael Love
43k • written 13 months ago by
bhandary.8590
▴ 20
0
votes
3
replies
827
views
DESeq2 - Comparing Comparisons
DESeq2
updated 13 months ago by
James W. MacDonald
68k • written 13 months ago by
sk
• 0
0
votes
4
replies
1.0k
views
lfcShrink and alpha value
lfcShrink
DESeq2
14 months ago • updated 13 months ago
anon
• 0
2
votes
1
reply
833
views
DESeq2- merging technical vs biological replicates
DESeq2
RNASeqData
updated 13 months ago by
Michael Love
43k • written 14 months ago by
Nikita
• 0
0
votes
2
replies
542
views
DESeq2 dispersion estimates fitting drastically different based on explanatory variable scaling
dispersionestimates
DESeq2
updated 13 months ago by
Michael Love
43k • written 13 months ago by
Sam
• 0
0
votes
2
replies
533
views
DESeq2 sizefactors counfounded with biological treatment
DESeq2
14 months ago
Anna
• 0
1
vote
2
replies
849
views
Deseq2 res Function
DESeq2
updated 14 months ago by
James W. MacDonald
68k • written 14 months ago by
sap275
▴ 10
0
votes
4
replies
815
views
Help with RSEM counts: do I transpose matrix?
RSEM
DESeq2
14 months ago
NB
• 0
0
votes
5
replies
808
views
Identical samples after deseq2 batch effect removal
DESeq2
updated 14 months ago by
Michael Love
43k • written 14 months ago by
maripane
• 0
0
votes
1
reply
627
views
DE analysis on Single cell RNA Seq time course data (using pseudotime) with 5 different genotypes
DESeq2
updated 14 months ago by
James W. MacDonald
68k • written 14 months ago by
Raya
• 0
1
vote
2
replies
532
views
Differene between the Wald and LRT test for time-series gene expression data
DESeq2
14 months ago
Denise
• 0
0
votes
1
reply
472
views
eRNAs count
RNASeq
DESeq2
updated 14 months ago by
ATpoint
★ 5.0k • written 14 months ago by
Irene
• 0
0
votes
1
reply
659
views
DESeq2 rerun returns different results
DESeq2
updated 14 months ago by
ATpoint
★ 5.0k • written 14 months ago by
wl1111
• 0
0
votes
1
reply
570
views
Is my time course analysis with DESeq2 valid?
GeneExpression
DESeq2
TimeCourse
updated 14 months ago by
Michael Love
43k • written 14 months ago by
Jason
• 0
0
votes
1
reply
698
views
DESeq2 "Not full rank" and "less than full rank" error
DESeq2
deseq
updated 14 months ago by
Guido Hooiveld
★ 4.1k • written 14 months ago by
Najla Abassi
• 0
2
votes
2
replies
1.2k
views
Handling biological replicates in DESeq2
DESeq2
updated 14 months ago by
ATpoint
★ 5.0k • written 14 months ago by
sk
• 0
5
votes
3
replies
29k
views
DESeq2: thousands of NA for pvalues?
deseq2
rna-seq
DEGs
cooks cutoff
written 7.0 years ago by
aishu.jp
▴ 30
1
vote
6
replies
1.7k
views
Volcano plot has many values around zero
RNA-seq
DESeq2
volcanoplot
updated 14 months ago by
Michael Love
43k • written 14 months ago by
nramakr
▴ 10
0
votes
2
replies
600
views
DESeq2 interaction between cancer cells and microenvironment cells
DESeq2
written 14 months ago by
ballackselma
• 0
4
votes
5
replies
1.5k
views
DESeq Input from Salmon + tximport/tximeta
tximportData
summarizeToGene
tximport
DESeq2
salmon
14 months ago
LuciaNhu
• 0
0
votes
1
reply
793
views
Average expression of genes across replicates
DESeq2
updated 14 months ago by
ATpoint
★ 5.0k • written 14 months ago by
James
• 0
0
votes
0
replies
466
views
News:
RNAseq data analysis with R/Bioconductor (4-15 November)
DESeq2
GenomicRanges
15 months ago
info
▴ 10
0
votes
11
replies
2.6k
views
Question about using LRT test for time course experiments with DESeq2
DESeq2
updated 9 months ago by
Rob
• 0 • written 15 months ago by
Denise
• 0
0
votes
1
reply
586
views
Deseq to output continous sequencing data
DESeq2
updated 15 months ago by
Michael Love
43k • written 15 months ago by
Neha
• 0
1
vote
4
replies
1.5k
views
Adjusted p-values become NA when sub-setting samples
DESeq2
15 months ago • updated 14 months ago
txema.heredia
• 0
3
votes
3
replies
1.7k
views
Robust way of dealing with low number of samples for Differential Gene Expression
limma
edgeR
DESeq2
MAST
written 15 months ago by
Satoshi
• 0
0
votes
2
replies
968
views
ESTMATE, MCPcounter, EPIC, xCell input
DESeq2
ESTIMATE
RNASeqData
MCPcounter
written 15 months ago by
Aleksandra
• 0
0
votes
1
reply
514
views
Should zero counts in some replicates lead to inflated p-values?
DESeq2
updated 15 months ago by
Michael Love
43k • written 15 months ago by
nicolettacommins
• 0
2
votes
3
replies
1.3k
views
Analysis of large RNA-Seq Datasets
DESeq2
updated 15 months ago by
Michael Love
43k • written 15 months ago by
KokiBio
• 0
1
vote
4
replies
1.2k
views
DESeq2 - interactions when using factors concatenated into group
DESeq2
15 months ago
boczniak767
▴ 740
0
votes
1
reply
411
views
2 factor analysis using DESeq2 but the result is not what I expected from the design
DESeq2
15 months ago
JY
• 0
0
votes
1
reply
499
views
How does deseq2 address intra group variance when calculating log2foldchange
DESeq2
updated 15 months ago by
Michael Love
43k • written 15 months ago by
agamemnon
• 0
0
votes
2
replies
538
views
how to extract expression matrix post deseq2
DESeq2
written 15 months ago by
agamemnon
• 0
0
votes
2
replies
573
views
Using Normalized Counts in DESeq2
DESeq2
updated 15 months ago by
Michael Love
43k • written 15 months ago by
KokiBio
• 0
3
votes
2
replies
675
views
When running contrasts, does it use normalized read counts?
DESeq2
15 months ago
Katherine
▴ 20
0
votes
1
reply
449
views
How to adjust the DESeq2 formula when there is no control group for one factor?
DESeq2
updated 15 months ago by
swbarnes2
★ 1.4k • written 15 months ago by
Bingqian
• 0
3
votes
4
replies
1.5k
views
Are transformed values from rlog /vst log2 normalized counts?
Normalization
DESeq2
rlog
vst
15 months ago
Eva
▴ 10
1
vote
4
replies
1.4k
views
Mostly increased counts in differentially accessible peaks using DESeq2
ATAC-seq
DESeq2
Oddresults
updated 15 months ago by
ATpoint
★ 5.0k • written 15 months ago by
Ricardo Iván
• 0
0
votes
1
reply
565
views
How to set filtering standard for low-expression genes in ImpulseDE2
DifferentialExpression
ImpulseDE2
DESeq2
updated 15 months ago by
Michael Love
43k • written 15 months ago by
Jiahao Tian
• 0
2
votes
4
replies
1.0k
views
RNASeq Time Course Question
DESeq2
RNASeq
TimeCourse
15 months ago
jihoon.kim.1495
▴ 10
0
votes
6
replies
1.5k
views
[DESeq2] Introduce a bias in DESeq2
DifferentialExpression
DESeq2
BatchEffect
updated 15 months ago by
Michael Love
43k • written 15 months ago by
CriticalPeriod
• 0
5
votes
4
replies
1.1k
views
How to control batch effect if Group and batch are same?
limma
DESeq2
BatchEffect
updated 15 months ago by
ATpoint
★ 5.0k • written 15 months ago by
Bioinformagician
• 0
0
votes
2
replies
757
views
RNA-Seq p value range variation
DESeq2
pvalue
RNA-Seq
15 months ago
paul.meraner
• 0
0
votes
0
replies
665
views
News:
RNAseq data analysis with Bioconductor (online course)
DESeq2
SummarizedExperiment
15 months ago
info
▴ 10
1
vote
6
replies
1.6k
views
heatmap with batch effect
DESeq2
BatchEffect
written 15 months ago by
tothuhien
• 0
4,845 results • Page
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TikTs33ssalpc
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This rotation style program sounds like a great way to explore different fields before committing to one. Similarly, apkgamex lets users ex…
Comment: Bioconductor Maintainer Application
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TikTs33ssalpc
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This compliance focused approach makes a lot of sense. Clear reminders and opt in policies help maintain trust, similar to how users look f…
Comment: Bile acid analysis with limpa
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ATpoint
★ 5.0k
Wilcox/Mann-Whitney-U cannot work because it is bound to sample size even when all of group X are lower than all of group Y. At 4 vs 3 the …
Comment: chemmineOB install help
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Jeremy
• 0
I could install ChemmineOB on my MacBook Pro M1 Max using this procedure **1. Install open-babel and eigen using homebrew and create a cus…
Comment: Making a Full Rank Model for Allele-Specific Expression with DESeq2
by
Gordon Smyth
53k
The design matrix that I described above is completely general. I imagine that it would be possible to use the same approach to generate fu…
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A: DESeq2: Is it possible to convert read counts to expression values via TPM and r
A: Filtering read counts matrix: how to deal with duplicated gene symbols, differen
Comment: limpa analysis advice
Answer: When to use edgeR or limma
Answer: When to use edgeR or limma
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