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Comment: DESeq2 testing ratio of ratios (RIP-Seq, CLIP-Seq, ribosomal profiling)
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Hi everyone.
I have a RIP-Seq analysis with 3 conditions and 3 assays (IP, IgG and Input for each of them). 4 replicates for each conditio…
Comment: Discrepancy between Excel TPP-TR reported values and the Melt Curve reported val
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You will need to provide a self-contained example, or it is unlikely that anybody could debug any possible issue.
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If you are fitting donor as a fixed effect you do not also need to fit as a random effect. The limma package fits a generalized least squar…
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Yes, perfectly valid. Analyzing counts from high-throughput experiments with DESeq2 (or edgeR, or limma) is standard. After all, what DiffB…
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AB
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Force=TRUE did the trick. Thank you.
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