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block
•
reset
4
votes
14
replies
6.2k
views
Including technical and biological replicates in Limma block analysis?
limma
microarray
block
paired
linear model
10.4 years ago
vas
▴ 40
6
votes
14
replies
3.7k
views
limma with continous covariate depending on gene, 2 block variables, pairing and technical replicates
limma
covariate
block
paired samples
technical replicates
updated 9.5 years ago by
Aaron Lun
★ 28k • written 9.5 years ago by
eleonoregravier
▴ 70
7
votes
7
replies
10k
views
batch effect : comBat or blocking in limma ?
batch effect
combat
limma
block
updated 9.6 years ago by
Ryan C. Thompson
★ 7.9k • written 9.6 years ago by
eleonoregravier
▴ 70
1
vote
6
replies
1.9k
views
DESEQ2 blocking design doesn't match expectation !
deseq2
block
pca
8.9 years ago
akp
• 0
3
votes
5
replies
2.1k
views
Limma contrast matrix question/concern
limma
block
design and contrast matrix
9.9 years ago
choofd
• 0
1
vote
4
replies
3.5k
views
Remove batch effect using individuals as random in the model in Limma
batch effect
Coeficents non estimable
block
random effects
limma design matrix
updated 7.5 years ago by
Aaron Lun
★ 28k • written 7.5 years ago by
mheydarpour
▴ 10
11
votes
4
replies
2.4k
views
LIMMA dupcor() function time issue.
limma
microarray
block
updated 20 months ago by
Gordon Smyth
52k • written 6.3 years ago by
tleona3
• 0
4
votes
3
replies
1.4k
views
Diffbind for regressing multiple confounding variables (blocking factors)
diffbind
block
regression
deseq2
chipseq
updated 4.5 years ago by
Rory Stark
★ 5.2k • written 5.6 years ago by
melnuesch
▴ 10
4
votes
3
replies
2.4k
views
Blocked design and Surrogate Variables Analsysis(SVA)
limma
sva
block
updated 3.2 years ago by
stuartthafass
• 0 • written 10.5 years ago by
kodream
▴ 20
2
votes
2
replies
1.6k
views
limma roast/mroast with patient-matched pre-/post- treatment
limma
roast
mroast
block
updated 8.9 years ago by
Ryan C. Thompson
★ 7.9k • written 8.9 years ago by
barbara.shih
• 0
0
votes
1
reply
1.7k
views
Test for differential variability with missMethyl (DiffVar) using block design
missmethyl
diffvar
design matrix
limma
block
updated 7.0 years ago by
lhuang7
▴ 50 • written 7.9 years ago by
Simone
▴ 190
0
votes
1
reply
1.3k
views
Limma: Correct for location and batch effect
limma
BatchEffect
duplicatecorrelation
block
batch
updated 4.5 years ago by
Kevin Blighe
★ 4.0k • written 4.5 years ago by
Katherine
• 0
12 results • Page
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Replies
Answer: Adding gene names as row annotations to an RNAseq heatmap made with ComplexHeatm
by
James W. MacDonald
68k
``` library(org.Dm.eg.db) rn <- select(org.Dm.eg.db, row.names(cent_expr_matrix), "SYMBOL","FLYBASE") ## ensure no NA values get propaga…
Comment: Discrepancies in normalised count data vs unnormalised
by
AMgroup
• 0
Thank you for your explanation, that makes sense. Do you have any suggestions on how to deal with this issue? if it is an issue? especially…
Comment: Announcing GBCC 2025: The first-ever joint conference between Bioconductor and G
by
nytwordlehints
• 0
I have always had a great fondness for games, so witnessing their transformation into animated versions is truly a delightful experience. […
Comment: FlowSOM is unable to create Output
by
EvangelinePope
• 0
I will remember it, Thank you so much for sharing.
Answer: Limma suqeezeVar
by
Gordon Smyth
52k
I am not quite clear why you're not using the limma package directly, because it will compute all the variances and the df automatically fo…
Votes
Deseq2 DE analysis of host-pathogen samples (model separately or jointly?)
Comment: Check removeBatchEffect effectiveness
Answer: Check removeBatchEffect effectiveness
A: what should I do with many zero counts from Salmon quantification
Comment: parameters of filterByExpr
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