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News:
Announcing the GBCC2025 Logo Design Contest!
Conference
Community
Galaxy
GBCC2025
Bioconductor
12 months ago
Maria Doyle
▴ 100
0
votes
2
replies
1.5k
views
DESeq2 analysis on Galaxy. PCA plot shows my replicates do not cluster and large intragroup variability
DESeq2
Galaxy
pca
updated 2.7 years ago by
swbarnes2
★ 1.4k • written 2.7 years ago by
mbxdn1
• 0
0
votes
1
reply
1.3k
views
Controlling for sex variance in Galaxy DESeq2
DESeq2
RNASeq
Galaxy
updated 3.7 years ago by
Michael Love
43k • written 3.7 years ago by
István
• 0
0
votes
3
replies
2.2k
views
StringTie output as Ballgown input
ballgown
StringTie
galaxy
rnaseq
updated 3.4 years ago by
Kevin Blighe
★ 4.0k • written 5.3 years ago by
lakshmi9c
• 0
0
votes
1
reply
792
views
deseq2 for enrichments between different subcellular fractions?
deseq2
galaxy
updated 5.5 years ago by
Michael Love
43k • written 5.5 years ago by
mikelirastor
• 0
1
vote
3
replies
1.8k
views
Running DESeq2 inside local Galaxy instance
deseq2
galaxy
8.5 years ago
gkuffel
▴ 10
0
votes
2
replies
1.7k
views
Data analysis in R
bioconductor
next-generation sequencing
galaxy
updated 8.8 years ago by
Lucas Schiffer
▴ 240 • written 8.8 years ago by
efraim.culfa
• 0
0
votes
4
replies
4.8k
views
Using DESeq2 inside Galaxy
rnaseq
galaxy
deseq2
updated 9.6 years ago by
Michael Love
43k • written 9.7 years ago by
Marcelo Pereira
▴ 70
0
votes
2
replies
1.5k
views
Inverting order of groups when using DESeq2
DESeq2
Galaxy
updated 9.6 years ago by
Michael Love
43k • written 9.6 years ago by
Marcelo Pereira
▴ 70
9 results • Page
1 of 1
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Comment: "Watched Tags" are being erased rather than updated
by
shepherl
4.2k
I can reproduce this and have opened an issue on the code base https://github.com/Bioconductor/support.bioconductor.org/issues/112
Comment: "Watched Tags" are being erased rather than updated
by
shepherl
4.2k
we can investigate this issue. thank you for bringing it to our attention
Comment: deseq2 filter the low counts
by
Michael Love
43k
> But within each comparison (contrast), if I trace some DEGs back to the original salmon merged gene counts (or even to normalised counts)…
Comment: deseq2 filter the low counts
by
empty_mt
• 0
Thank you for you suggestion to look at the pre-filtering section in the DESeq2 vignettes - am I missing something? I have 6 treatments, …
Comment: deseq2 filter the low counts
by
Michael Love
43k
Have you seen this part: https://bioconductor.org/packages/release/bioc/vignettes/DESeq2/inst/doc/DESeq2.html#pre-filtering
Votes
Comment: "Watched Tags" are being erased rather than updated
Comment: deseq2 filter the low counts
Answer: DESeq2 interactions
Answer: Important: Bioconductor Release 3.22 Delayed
A: Multi-level experiments in Limma
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