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spikein
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366
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Spike-in normalization method in ATACseq
sperm
Drosophila_melanogaster
ATACSeq
Normalization
SpikeIn
updated 6 weeks ago by
ATpoint
★ 4.6k • written 7 weeks ago by
Katerina
• 0
0
votes
2
replies
356
views
Analysis of transcription inhibition with DESeq2
SpikeIn
DESeq2
Normalization
written 4 months ago by
ADopico
• 0
0
votes
1
reply
498
views
Problem with spike-ins (ERCC) in DESeq2 analysis after doing RUVg!
SpikeIn
RUVSeq
DESeq2
RUV
updated 5 months ago by
Michael Love
43k • written 5 months ago by
Ειρήνη
• 0
0
votes
0
replies
281
views
Diffbind Error in socketConnection
DiffBind
Errorinsocketconnection
SpikeIn
8 months ago
Virangika
• 0
1
vote
2
replies
779
views
DiffBind spike-in lib.sizes confusion
SpikeIn
DiffBind
21 months ago
Weisheng
• 0
6
votes
2
replies
3.0k
views
Clarification of what DESeq2::estimateSizeFactors controlGenes does and when it should *not* be used
DESeq2
SpikeIn
Normalization
DifferentialExpression
updated 22 months ago by
ATpoint
★ 4.6k • written 22 months ago by
kalavattam
▴ 10
0
votes
2
replies
2.2k
views
How to use spike-in information (sequences from another species) with DESeq2::DESeq()
DESeq2
SpikeIn
Normalization
updated 7 months ago by
maria.soler
• 0 • written 23 months ago by
kalavattam
▴ 10
3
votes
4
replies
2.3k
views
RiP RLE normalisation using spike-in peaks in DiffBind for ChIP-seq
DiffBind
SpikeIn
Normalization
ChIP-seq
csaw
2.0 years ago • updated 23 months ago
spg
• 0
0
votes
0
replies
1.1k
views
DiffBind spike-in normalisation with varying amounts of spike-in chromatin
DiffBind
ChIPSeq
Normalization
SpikeIn
3.2 years ago
Drew
• 0
0
votes
0
replies
889
views
Spike-In Cells for Normalization?
SpikeIn
SingleCell
3.3 years ago
mb1996
• 0
0
votes
2
replies
1.4k
views
Using both spike in and TMM normalizations in ChIP-seq samples
ChIPSeq
SpikeIn
edgeR
Normalization
3.4 years ago
maria.soler
• 0
7
votes
12
replies
4.7k
views
Using edgeR and a spike-in to calculate absolute abundance
edgeR
SpikeIn
RNASeq
updated 14 months ago by
Miguel
• 0 • written 3.8 years ago by
robert.chen
• 0
1
vote
9
replies
3.7k
views
Spike-in normalization in EdgeR
CUTandRUN
edgeR
Normalization
SpikeIn
ChIPSeq
updated 2.1 years ago by
Bogdan
▴ 670 • written 4.1 years ago by
Hesh
▴ 10
13 results • Page
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Comment: Biostrings: Error writing long reads (>200 kbps) with writeQualityScaledXStringS
by
Aidan
▴ 60
Quick update: this is fixed in a branch awaiting PR to Biostrings (https://github.com/Bioconductor/Biostrings/pull/122). Should be in `deve…
Answer: Can DESeq2's design compensate for sequencing experimental design shortcomings?
by
Michael Love
43k
For statistical analysis plans, I recommend working with a local statistician or someone familiar with linear models in R. I have to reserv…
Comment: how should I apply "cpg.annotate" to TCGA methylation data in hg38 for HM450K?
by
xiaofeiwang198266
• 0
Thanks for your reply!
Comment: Issues with seqlevelsStyle when making custom txdb objects for genomes/annotatio
by
Hervé Pagès
16k
> I have requested gDNAx developers for a feature request to turn off seqlevelsStyle(txdb) Yes, that's the right course of action. `seqlev…
Comment: ISO a solution to rank-order introns by transcript
by
mat149
▴ 80
Hey, In revisiting this question, the following code works for the '+' strand. I am having issues with demarcating intron rank by transcri…
Votes
C: edgeR and lack of counts ID on CPM matrix
A: DESeq2 Following RSEM
A: importing RSEM data into DESeq2
A: Expected counts from RSEM in DESeq2
A: Using RSEM reads for DESeq2
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