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spikein
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Spike-in normalization method in ATACseq
sperm
Drosophila_melanogaster
ATACSeq
Normalization
SpikeIn
updated 14 months ago by
ATpoint
★ 5.0k • written 14 months ago by
Katerina
• 0
0
votes
2
replies
729
views
Analysis of transcription inhibition with DESeq2
SpikeIn
DESeq2
Normalization
written 17 months ago by
ADopico
• 0
0
votes
1
reply
1.1k
views
Problem with spike-ins (ERCC) in DESeq2 analysis after doing RUVg!
SpikeIn
RUVSeq
DESeq2
RUV
updated 18 months ago by
Michael Love
43k • written 18 months ago by
Ειρήνη
• 0
0
votes
0
replies
569
views
Diffbind Error in socketConnection
DiffBind
Errorinsocketconnection
SpikeIn
21 months ago
Virangika
• 0
1
vote
2
replies
1.1k
views
DiffBind spike-in lib.sizes confusion
SpikeIn
DiffBind
2.9 years ago
Weisheng
• 0
6
votes
2
replies
4.4k
views
Clarification of what DESeq2::estimateSizeFactors controlGenes does and when it should *not* be used
DESeq2
SpikeIn
Normalization
DifferentialExpression
updated 3.0 years ago by
ATpoint
★ 5.0k • written 3.0 years ago by
kalavattam
▴ 10
0
votes
2
replies
3.1k
views
How to use spike-in information (sequences from another species) with DESeq2::DESeq()
DESeq2
SpikeIn
Normalization
updated 20 months ago by
maria.soler
• 0 • written 3.0 years ago by
kalavattam
▴ 10
3
votes
4
replies
3.3k
views
RiP RLE normalisation using spike-in peaks in DiffBind for ChIP-seq
DiffBind
SpikeIn
Normalization
ChIP-seq
csaw
3.1 years ago • updated 3.0 years ago
spg
• 0
0
votes
0
replies
1.4k
views
DiffBind spike-in normalisation with varying amounts of spike-in chromatin
DiffBind
ChIPSeq
Normalization
SpikeIn
4.3 years ago
Drew
• 0
0
votes
0
replies
1.1k
views
Spike-In Cells for Normalization?
SpikeIn
SingleCell
4.4 years ago
mb1996
• 0
0
votes
2
replies
2.0k
views
Using both spike in and TMM normalizations in ChIP-seq samples
ChIPSeq
SpikeIn
edgeR
Normalization
4.5 years ago
maria.soler
• 0
7
votes
12
replies
6.2k
views
Using edgeR and a spike-in to calculate absolute abundance
edgeR
SpikeIn
RNASeq
updated 2.3 years ago by
Miguel
• 0 • written 4.9 years ago by
robert.chen
• 0
1
vote
9
replies
5.1k
views
Spike-in normalization in EdgeR
CUTandRUN
edgeR
Normalization
SpikeIn
ChIPSeq
updated 3.2 years ago by
Bogdan
▴ 670 • written 5.2 years ago by
Hesh
▴ 10
13 results • Page
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Comment: Making a Full Rank Model for Allele-Specific Expression with DESeq2
by
Gordon Smyth
53k
The design matrix that I described above is completely general. I imagine that it would be possible to use the same approach to generate fu…
Comment: Making a Full Rank Model for Allele-Specific Expression with DESeq2
by
chp265
• 0
Cool, glad to hear I wasn't missing some functionality in DESeq2. I don't know that much about the differences between DESeq2 vs edgeR; my …
Comment: Making a Full Rank Model for Allele-Specific Expression with DESeq2
by
Michael Love
43k
Yes, so for one thing, note that Gordon has provided code for doing what you are asking in edgeR, which you can go ahead and use. You aske…
Comment: Making a Full Rank Model for Allele-Specific Expression with DESeq2
by
chp265
• 0
I don't think DESeq2 has the ability to do multi-contrast comparisons, at least based on what I can understand of the manual; I can pick mu…
Comment: Upcoming Ensembl API and data access changes - new blog post available
by
Hugo Gruson
• 0
Thanks for the update. To be very explicit: what is going to happen to the Biomart service, on which the biomaRt bioconductor package relie…
Votes
A: DESeq2: Is it possible to convert read counts to expression values via TPM and r
A: Filtering read counts matrix: how to deal with duplicated gene symbols, differen
Comment: limpa analysis advice
Answer: When to use edgeR or limma
Answer: When to use edgeR or limma
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