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spikein
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849
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Spike-in normalization method in ATACseq
sperm
Drosophila_melanogaster
ATACSeq
Normalization
SpikeIn
updated 10 months ago by
ATpoint
★ 4.9k • written 10 months ago by
Katerina
• 0
0
votes
2
replies
602
views
Analysis of transcription inhibition with DESeq2
SpikeIn
DESeq2
Normalization
written 12 months ago by
ADopico
• 0
0
votes
1
reply
890
views
Problem with spike-ins (ERCC) in DESeq2 analysis after doing RUVg!
SpikeIn
RUVSeq
DESeq2
RUV
updated 14 months ago by
Michael Love
43k • written 14 months ago by
Ειρήνη
• 0
0
votes
0
replies
487
views
Diffbind Error in socketConnection
DiffBind
Errorinsocketconnection
SpikeIn
17 months ago
Virangika
• 0
1
vote
2
replies
967
views
DiffBind spike-in lib.sizes confusion
SpikeIn
DiffBind
2.5 years ago
Weisheng
• 0
6
votes
2
replies
4.1k
views
Clarification of what DESeq2::estimateSizeFactors controlGenes does and when it should *not* be used
DESeq2
SpikeIn
Normalization
DifferentialExpression
updated 2.6 years ago by
ATpoint
★ 4.9k • written 2.6 years ago by
kalavattam
▴ 10
0
votes
2
replies
2.8k
views
How to use spike-in information (sequences from another species) with DESeq2::DESeq()
DESeq2
SpikeIn
Normalization
updated 16 months ago by
maria.soler
• 0 • written 2.6 years ago by
kalavattam
▴ 10
3
votes
4
replies
2.9k
views
RiP RLE normalisation using spike-in peaks in DiffBind for ChIP-seq
DiffBind
SpikeIn
Normalization
ChIP-seq
csaw
2.7 years ago
spg
• 0
0
votes
0
replies
1.3k
views
DiffBind spike-in normalisation with varying amounts of spike-in chromatin
DiffBind
ChIPSeq
Normalization
SpikeIn
3.9 years ago
Drew
• 0
0
votes
0
replies
1.1k
views
Spike-In Cells for Normalization?
SpikeIn
SingleCell
4.0 years ago
mb1996
• 0
0
votes
2
replies
1.8k
views
Using both spike in and TMM normalizations in ChIP-seq samples
ChIPSeq
SpikeIn
edgeR
Normalization
4.1 years ago
maria.soler
• 0
7
votes
12
replies
5.8k
views
Using edgeR and a spike-in to calculate absolute abundance
edgeR
SpikeIn
RNASeq
updated 23 months ago by
Miguel
• 0 • written 4.5 years ago by
robert.chen
• 0
1
vote
9
replies
4.5k
views
Spike-in normalization in EdgeR
CUTandRUN
edgeR
Normalization
SpikeIn
ChIPSeq
updated 2.8 years ago by
Bogdan
▴ 670 • written 4.9 years ago by
Hesh
▴ 10
13 results • Page
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Comment: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
Michael Love
43k
I moved your post from an "answer" to a "comment". I don't know about `offst()` function, what is the scale or direction, or whether you a…
Comment: Average biological replicates in RNA-seq
by
boczniak767
▴ 740
@atpoint Should I first standardise data and then average replicates?
Comment: 3-factor DESeq2 trime-series analysis
by
gogeni5529
• 0
Thans you @swbarnes2 for the answer. I can see why trying to include all three parameters might be difficult to interpret. But we're inte…
Comment: 3-factor DESeq2 trime-series analysis
by
swbarnes2
★ 1.4k
What does your PCA look like? A lot of times, I find that different cell lines are so different from each other, that they should not be d…
Comment: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
yongjie.wang
• 0
Thanks for your reply. " normFactors <- exp(-1 * offst(set2)) normFactors <- normFactors / exp(rowMeans(log(normFactors))) normalizat…
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Answer: Limpa Pipeline Order
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Answer: ROAST compare results with complex design
How to account for solvent controls in DESeq2 when comparing follicular vs luteal phase?
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