I am a newer to use Bioconductor; I have met the following question when I load data by WTChimeraData() function This is my codes:

library(MouseGastrulationData)
sce.chimera <- WTChimeraData(samples=5:10)

sometimes, returned this

sce.chimera <- WTChimeraData(samples=5:10) |======================================================================| 100%

Error in curl::curl_fetch_memory(url, handle = handle) : Timeout was reached: [experimenthub.bioconductor.org] Connection time-out

and sometimes, returned this

Error in value[3L] : failed to connect reason: Timeout was reached: [bioconductor.org] Connection time-out Consider rerunning with 'localHub=TRUE' In addition: Warning message: Could not check database for updates. Database source currently unreachable. This should only be a temporary interruption. Using previously cached version.

I don't know why, who can help me? and thank you !

Looks to me like you're struggling to connect to the Bioconductor servers that hold the data.

If you have downloaded the data before, it looks like you can use the argument localHub=TRUE to avoid connecting to the servers.

If you haven't, then you could try running the code again now, after a little time has passed since the first attempt. Hopefully the servers just had some temporary downtime. If that doesn't work, then it seems like you have something set up on your network that is blocking that connection.

Jonny