Bioconductor Forum

else asks.... ;-) ... subscription to a variety of useful mailing lists, including microarray-norm@ebi.ac.uk, is available here http://www.mged.org/Workgroups/index.html In fact, there's a whole host of useful stuff on

updated 21.8 years ago • michael watson IAH-C

Any help or suggestions would be greatly appreciated! Thanks so much, Norm Norm Allaire Scientist II Genetics and Genomics Biogen Idec 14 Cambridge Center Cambridge, MA 02142 Office: 617-679-2932

HTqPCR HTqPCR

updated 11.9 years ago • Norm Allaire

<div class="preformatted">Hi, I'm new to R. I'm trying to analyze 8 Fluidgm 96 *96 plates (96 assays and 192 samples replicated 4 times). I downloaded and installed HTqPCR and I'm now trying to read in the raw data. I am using the "HTqPCR -high-throughput qPCR analysis in R and Bioconductor" April 14, 2011 as a guide but the example in section 13 is for 48*48 arrays. I tried to modify the …

qPCR qPCR

updated 11.9 years ago • Norm Allaire

CONV F 1816 LOW CONV M 1818 LOW CONV F 1822 LOW CONV M 1826 LOW CONV F 1828 LOW CONV F 1764 NORM CONV F 1770 NORM CONV F 1782 NORM CONV M 1792 NORM CONV M 1802 NORM CONV F 1804 NORM CONV F 1755 HIGH HCHF M 1767 HIGH HCHF F 1777...HCHF F 1783 LOW HCHF M 1785 LOW HCHF M 1807 LOW HCHF F 1821 LOW HCHF M 1827 LOW HCHF F 1761 NORM HCHF F 1763 NORM HCHF F 1769 NORM HCHF M 1789 NOR…

deseq2

updated 6.9 years ago • sharmila.ahmad

colnames(design) <- c("s1", "s2") #process bg <- backgroundCorrect(agi, method = "normexp") norm <- normalizeBetweenArrays(bg, method = "cyclicloess") avg <- avereps(norm, ID=norm$genes$ProbeName) #fit contmat <- makeContrasts...applies subtraction background correction with limma bg_subtract <- function(x){ return(list(norm = x$norm, …

Annotation Normalization vsn limma Annotation Normalization vsn limma

updated 12.8 years ago • Cornish, Joseph NIH/NIAID [F]

lt;- info$ID libsizes plot(indiff_count) indiff_norm <- dba.normalize(indiff_count) norm <- dba.normalize(indiff_count, bRetrieve=TRUE) norm normlibs <- cbind(FullLibSize=norm$lib.sizes, NormFacs=norm$norm.factors...NormLibSize=round(norm$lib.sizes/norm$norm.factors)) rownames(normlibs) <- info$ID normlibs #Continue with DiffBind to obtain differenti…

edgeR Normalization DiffBind

updated 4.6 years ago • Nico

Code: <pre> AF.mydata <- ReadAffy() AF.esetRMA<-rma(AF.mydata) AF.sampletype <- c('Norm','Norm','Norm','Mut','Mut','Mut','Mut',) AF.group <-factor(AF.sampletype) AF.fit <- lmFit(AF.esetRMA,AF.design) AF.contrast.matrix...lt;-makeContrasts(Norm-Mut,levels=AF.design) AF.fit2<- contrasts.fit(AF.fit,AF.contrast.matrix) AF.fit2 <- eBayes…

Limma SAM differential expression

updated 11.1 years ago • Bade

I do not get best results with cosine distance, which is the usual distance measure I choose. L1 norm, L2 norm, L3 norm all seem to work better, but I am not sure which one is the best and I could not think of a theoretical justification

MAST

updated 8.3 years ago • alexanderaivazidis

An embedded and charset-unspecified text was scrubbed... Name: not available Url: https://stat.ethz.ch/pipermail/bioconductor/attachments/20061005/ 78ddec9f/attachment.pl

updated 19.3 years ago • James Anderson

Hi Gordon, I used to use predFC() to get modified log count-per-million values per sample but now I'm switching to cpm(). I just realized that predFC() doesn't use the normalization factors in the DGEList object when design=NULL, but it does appear to use them when the design is specified (see example below) and there is no argument to specify them, unlike cpm(). Is this a bug or the intended b…

Normalization Normalization

updated 12.4 years ago • Jenny Drnevich

in edgeR are: library(edgeR) dge <- DGEList(counts = counts, group = group ) norm <- calcNormFactors(dge,method="TMM") d <- estimateGLMCommonDisp(norm, design = design) d <- estimateGLMTrendedDisp(d,design...log(counts)[,1], where i is the index of each individual (each row in the count matrix). Then I used norm&am…

Normalization edgeR Normalization edgeR

updated 11.4 years ago • Zhan Tianyu

3Dlist(maNormMed()),f.scale=3Dlist(maNormMAD()) ) giv= e a different result from using y=3DmaNorm(x,norm=3D=B2median=B2) and z=3DmaNormScale(y,norm=3D=B2globalMAD=B2) serially? Thanks, Sean [[alternative HTML version deleted]] </div

updated 22.0 years ago • Sean Davis

expressions (rows) and the output (norm). *data* GSM276723.CEL GSM276724.CEL GSM276725.CEL GSM276726.CEL norm 0.897000 0.590000 0.683000 0.949000 206427_s_at...5.903681 5.658115 GSM276732.CEL GSM276735.CEL GSM276736.CEL GSM276737.CEL norm 0.43400 0.647000 0.113000 1.000000 206427_s_at 12.80257 5.645002…

updated 16.2 years ago • Eleni Christodoulou

<div class="preformatted">Dear all, 1- In normalizeCtData for norm="geometric.mean" , Can we average over all arrays or should we obrain the geometric mean of all samples? 2- geo.mean.ref= apply...div class="preformatted">Dear all, 1- In normalizeCtData for norm="geometric.mean" , Can we average over all arrays or should we obrain the geometric mean of all samples? 2- geo.mean.ref...bu…

updated 13.8 years ago • Ali Mohammadian

Hi, I have 2 bed files from 2 control samples that have single nucleotide reads over a region. I would like to calculate the average coverage (and standard deviation) between the 2 samples at each base. I have calculated the size factors for each library to normalize the reads before averaging. I have also extended the reads by 100 basepairs to smooth out the coverage. I can easily make bigwigs …

GenomicRanges

updated 5.1 years ago • Jake

Chromosome #Setup design (from snapCGH guide) stuff$design <- c(-1,-1) #Normalisation within normed <- normalizeWithinArrays(stuff, method="loess") #Get rid of "genes" with no location information normed$genes <- normed...genes[!(is.na(normed$genes$Chr)) & normed$genes$Chr != "",] #ProcessCGH normed2 <- processCGH(normed, method.of.averaging = mean,ID="Prob…

Cancer snapCGH Cancer snapCGH

updated 18.9 years ago • Daniel Brewer

y<-dt(x1[1:(n.points/2)],DOF[j]) y<-c(y,rev(y)) y/sum(y)/Norm }) Tmp<-multi_conv(PDF) Tmp = Tmp*(n.points-1)/MaxDiff[i]/2 }else{ warning(paste("Gene set: (index ",i, ") has 0 overlap with eset.",sep="")) Tmp...if(!silent & i%%5==0){cat(".")} Indexes = geneSets[[i]] if(length(Indexes)!=0){ N…

qusage

updated 11 months ago • oliverd

<div class="preformatted"> I have a custom Affy array which allows several applications (expression profiling, genotyping, etc...) on a single chip. I want to use RLMM to analyze our genotyping data, but have a couple of questions: 1) Instead of normalizing to the scale of the training set (which I don't have), does it make sense to normalize all arrays to each other using quantile normal…

GO Classification cdf affy RLMM GO Classification cdf affy RLMM

updated 19.4 years ago • Amit Bahl

geo/query/acc.cgi?acc=GSM803742  is it rma wich converts it too log2 like this: > norm <- rma(raw); expr <- exprs(norm); >Pda <- fitPLM(raw, model = PM ~ -1 + probes + samples) Thanks, Anna &nbsp

affy microarray bioinformatics

updated 10.9 years ago • kanacska

preformatted">dear all: i have two questions 1. is the "size factor" from DESeq equally to the "norm factor" in the edgeR 2. i can get the normalized counts by counts(obj,normalized=T) how to get normalized counts from edgeR...can i use the each col of count matrix divided by norm factor -- shan gao Room 231(Dr.Fei lab) Boyce Thompson Institute Cornell University Tower Road, Ithaca, NY 14…

DESeq DESeq

updated 13.5 years ago • wang peter

FALSE, background=T, library=DBA_LIBSIZE_BACKGROUND, normalize=DBA_NORM_LIB) db_data_spikeinNorm2$norm$DESeq2$lib.sizes [1] 7424321 7030471 8640826 7006223 > db_data_spikeinNorm2$norm$background$binned$totals [1] 7424321...TRUE, background=T, library=DBA_LIBSIZE_BACKGROUND, normalize=DBA_NORM_LIB) db_data_spikeinNorm3$norm$DESeq2$lib.sizes [1] 7747122 7334460 9112179 7386926 db_data_sp…

SpikeIn DiffBind

updated 2.8 years ago • Weisheng

<div class="preformatted">Hello, I'm new to marray, and after reading all of the tutorials and playing around with my data, I find myself in need of a bit of advice. I am attempting to load a batch of GenePix files and conduct a loess normalization on the unflagged spots. I'm having no problem normalizing the data using the approach below, but I am unsure whether the weight matrix is bei…

marray marray

updated 19.6 years ago • Oliver R. Homann

swirl yy <- maNormMain(x,f.loc=list(maNormMed()),f.scale=list(maNormMAD())) z1 <- maNorm(x, norm="m") z2 <- maNormScale(z1, norm="globalMAD") par(mfrow=c(2,2)) for(i in 1:4) plot(maM(z2)[,i], maM(yy)[,i]) Could you described a little more how

updated 22.0 years ago • Jean Yee Hwa Yang

Hi all, I have a question related to ChIP-seq spike-in normalization as illustrated on the csaw vignette. The idea of calculating a norm factor on the spikes data is great. But as mentionned in the vignette, using the normoffset function on the spike (...) _assume that the library sizes are the same between spike.data and endog.data_ (...). However, in my case, I did two seperate mappings (one…

ChIP-seq csaw

updated 7.2 years ago • Nicolas Servant

offset=1) boxplot(as.data.frame(Enorm$E), main="Mean intensities - normexp") #Normalisation quantile NormE <- normalizeBetweenArrays(Enorm,method="quantile") boxplot(as.data.frame(NormE$E), main="Normalized intensities") NormE...E <- log2(NormE$E) pData <- data.frame(population = c('mal','mal', 'mal', 'mal','mal','mal','mal','mal','mal','mal','mal','mal','mal','mal','sai n'…

updated 14.4 years ago • bigoun

to get stuck in a loop marrayNorm 1.1.1 > unix.time(experiment1.norm<-maNorm(experiment1, norm="loess")) [1] 803.99 29.73 843.40 0.00 0.00 marrayNorm 1.1.3 (installed with bioconductor 1.2) > unix.time(experiment1.norm...lt;-maNorm(experiment1, norm="loess")) Timing stopped at: 8874.73 19.65 10289.06 0 0 ie I interrupted the process. is there a known problem with …

PROcess PROcess

updated 22.5 years ago • Rob Dunne

value after > normalization?? > Min Normalized : ???? > Max Normalized : ???? > StdErr Norm : ???? > StdDev Norm : ???? > t-test P-value : P-value > Flags [A,M,P] : A - Absent; P - Present ; M - ??? > Raw : ???? > Min Raw : ???? > Max Raw : ???? > StdErr Raw : ???? &a…

Normalization Normalization

updated 20.9 years ago • S Peri

1.1.3 (installed with bioconductor 1.2) > unix.time(experiment1.norm<-maNorm(experiment1, norm="loess")) Timing stopped at: 8874.73 19.65 10289.06 0 0 ie I interrupted the process - but with marrayNorm 1.1.1 reinstalled...marrayNorm 1.1.1 > unix.time(experiment1.norm<-maNorm(experiment1, norm="loess")) [1] 803.99 29.73 843.40 0.00 0.00 is there a known proble…

PROcess PROcess

updated 22.5 years ago • Rob Dunne

or I am doing something wrong? I have tried the following normalizations: 1. normp <-maNorm(raw, norm="p") 2. normg<-maNormScale(normp, norm ="g") In each case the scatter plot yields a slope of .5 rather than 1. Any suggestions would

Normalization Cancer marray Normalization Cancer marray

updated 21.4 years ago • Richard Friedman

1000, lc = 0) myTMM myfilt = filtered.data(countsTable, factor = conditions$Cellular\_Type, norm = FALSE, depth = NULL, method = 1, cv.cutoff = 100, cpm = 1, p.adj = "fdr") mynoiseq = noiseqbio(mydata, k = 0.5, norm = "tmm", factor="Cellular\_Type

noiseq logfoldchange

updated 7.7 years ago • Aurora

Context: I've been doing some in silico research involving large-scale simulations with various DGE packages. I detected an error (detailed below) in my simulations that seemed new to edgeR 4.2.1 and did not occur in earlier versions. The following example might involve some non-sensical data, but I was able to reduce the original, meaningful, large-scale simulation dataset to this 3 by 3 one f…

edger edgeR

updated 15 months ago • Hongjian

colnames(design) <- c("s1", "s2") #process bg <- backgroundCorrect(agi, method = "normexp") norm <- normalizeBetweenArrays(bg, method = "cyclicloess") avg <- avereps(norm, ID=norm$genes$ProbeName) #fit contmat <- makeContrasts...bg = bg, desg = desg, norm = norm) processed <- matrix(NA, nrow = …

miRNA Annotation Normalization Visualization Clustering Infrastructure Cancer BSgenome vsn

updated 12.8 years ago • Matthew Thornton

access the slot 'count'. After fixing that, it runs into: Error in qpHexbin(mnorm, main = "MA-Plot :: Norm") : couldn't find function "hexagons" Error in qpHexbin(mnorm, main = "MA-Plot :: Norm") : couldn't find function "hex.legend" Which I also

hexbin arrayQuality hexbin arrayQuality

updated 20.6 years ago • Rob J Goedman

normalizations in a row with the default. That is to say I have done: ira.norm <- maNorm(ira.raw, norm = "p") ira.x2.norm <- maNorm(ira.norm, norm = "p") The curves flattened considerably. Is there a potenial problem with this procedure

Normalization Cancer Normalization Cancer

updated 21.9 years ago • Richard Friedman

I would like to take out raw counts from __gene X __from matrix __A__, and use the lib-size and norm-factors from a DGEList-object based on matrix __B__. How do I calculated the normalized expression of gene X? Thanks, &nbsp

limma

updated 7.6 years ago • R

j in 1:(i - 1)) { mm <- subdata[, c(i, j)] rs5 <- rowSums(mm) > 5 norm <- t(t(mm)/colSums(mm)) * gm(colSums(mm)) delta <- optimize(myFun, interval = c(1e-04, 0.99), tol = 1e-06, maximum = TRUE, y = norm[rs5, ], der = 0, doSum

Normalization Normalization

updated 13.2 years ago • Guest User

data analysis, for that I am using a matrix of log Fold change values generated by: <pre> norm <- assay(normTransform(dds)) i <- seq.int(1L,72,by = 2L) # 72 is the total number of samples. norm.fc <- norm[,i]-norm[,i+1] write.table

RNA-Seq deseq2 batch effect

updated 9.3 years ago • g.atla

function 'featureNames' for this call This information is available in the marrayNorm object: > norm at maGnames@maInfo[1:10,] ID Name X22170 22170 DXF68S1E X21642 21642 ABCA2 X22209 22209 LOC56990 X22121 22121 PDZD2 X24284...GGA2 X22739 22739 FLJ14566 I can fix the gene names by doing the following geneNames(monkey) <- norm at maGnames@maInfo$Name…

Cancer probe convert Cancer probe convert

updated 19.5 years ago • Daniel Brewer

<div class="preformatted">Hello, I'm not sure if this is a known issue, but I have been experiencing unpredictable results with the warpSet function in flowStats (see sessionInfo at end). Essentially, I have a been experimenting with warpSet and have it in a script that runs through the same 5 fcs files as a flowSet each time. About every 2 out of 3 runs I get this error with warpSet: par…

flowStats flowStats

updated 15.6 years ago • Aric Gregson

library(reb) expr <- justRMA() matrix <- assayData(expr)$exprs #Define oncocytoma as norms norms <- 16:30 chr <- buildChromLocation.2("hgu133plus2.db","bands") cset <- reb(matrix,chr,ref=norms,center=TRUE) exprs...lt;- cset[,-norms] exprs[abs(exprs) < 1.96] <- NA banded <- cset2band(exprs,chr,FUN=mean,na.rm=TRUE) h.cyto <- bui…

Organism hgu133plus2 reb Organism hgu133plus2 reb

updated 15.5 years ago • Sathe, Tejas

dgelist <- DGEList(matrix) ##the matrix with gene names as row names and counts in columns norm-mat <- calcNormFactors(dgelist, method = "TMM") norm-mat <- cpm(norm-mat, log = TRUE) For DESeq2: DESeq2 requires integers as input

DESeq2 tximport Kallisto TMM edgeR

updated 4.2 years ago • BioNovice247

1 dis 2 2A7_Hex_07Jul_09.CEL 1 dis 3 2A8_Hex_07Jul_09.CEL 1 dis 4 2A9_Hex_07Jul_09.CEL 1 norm 5 2AA_Hex_07Jul_09.CEL 1 norm 6 2AB_Hex_07Jul_09.CEL 1 norm </div

limma sva limma sva

updated 14.1 years ago • Fabrice Tourre

installed with bioconductor 1.2) > > unix.time(experiment1.norm<-maNorm(experiment1, norm="loess")) >Timing stopped at: 15358.18 19.85 20734.47 0.00 0.00 > >ie I interrupted the process - >but with marrayNorm...reinstalled > > marrayNorm 1.1.1 > > unix.time(experiment1.norm<-maNorm(experiment1, norm="…

limma PROcess limma PROcess

updated 22.3 years ago • Rob Dunne

Read 23148733 records Read 21898540 records [0% done] Starting chr:80000-1e+05:Error in var(tr$"+"$norm[x[1]:x[2]]) :    error in evaluating the argument 'x' in selecting a method for function 'var': Error in tr$"+"$norm[x[1]:x[2]] :  &nbsp

bayespeak

updated 10.4 years ago • Reed

FALSE, quantile=0.9, groups =files$Group, verbose=TRUE) s.norm <- normalizeCtData(raw.cat, norm="scale.rank") exprs(s.norm) write.table(exprs(s.norm),file="Ct norm scaling.txt") g.norm <- normalizeCtData(raw.cat, norm...geometric.mean") exprs(g.norm) write.table(exprs(g.norm),file="Ct norm media geometrica.txt") Now if we look at the content of the two expression value fil…

Normalization HTqPCR Normalization HTqPCR

updated 12.3 years ago • Guest User

FALSE, quantile=0.9, groups =files$Group, verbose=TRUE) s.norm <- normalizeCtData(raw.cat, norm="scale.rank") exprs(s.norm) write.table(exprs(s.norm),file="Ct norm scaling.txt") g.norm <- normalizeCtData(raw.cat, norm...geometric.mean") exprs(g.norm) write.table(exprs(g.norm),file="Ct norm media geometrica.txt") Now if we look at the content of the two expression value fil…

Normalization HTqPCR Normalization HTqPCR

updated 12.3 years ago • Guest User

metagenomeSeq's function "aggTax" to aggregate:</span>     AggObj = aggTax(MRcounts(obj, norm=TRUE, log=FALSE),norm=TRUE, log=FALSE, lvl = Class, out = "MRexperiment") As you can see, I am using the normalized counts for aggregation

metagenomeSeq microbiome

updated 10.9 years ago • noelle.noyes

handy later on. The parts that should interest you most are the highcut and lowcut options. The normed and rawout options are not used that often. I last ran this code under R 2.6.1, so I am not sure if it will work without a...0, method = "loess", lowcut=0, highcut=195000, bw = 0.1, rawout=FALSE,normed=FALSE, SpecNames = list.files(fldr, patter…

PROcess PROcess

updated 16.1 years ago • Davis, Wade

1 print tip is present), but it did end up making the code crash. This can be fixed by specifying norm='l' (if loess normalization is wanted) in the agQuality. I think that should be the default in agQuality and change (in agQuality...defs <- list(norm = "p") to defs <- list(norm = "l") As a note, the diagnostic spatial images being produced are not correct either. It prod…

Normalization Biobase hexbin arrayQuality marray Normalization Biobase hexbin arrayQuality

updated 20.1 years ago • Paquet, Agnes

the group representing each individual chipseq run, and then creating a design matrix to calculate norm factors, estimate dispersion, and perform glmQLFtests (following p.8 of the edgeR manual

diffbind edger

updated 7.9 years ago • james.dalgleish

address 0x110660000, cause 'memory not mapped' Traceback: 1: .Call("bayespeak", as.integer(tr$"+"$norm[659981:720020]), as.integer(tr$"-"$norm[659981:720020]), as.integer(w$norm[659981:720020]), as.double(w$norm[659981:720020]), as.integer...max(w$norm[659981:720020])), as.integer(60000), as.character(ch), start = as.integer(83925925), end = as.integer(89929925), as.integer(bin.size

BayesPeak BayesPeak

updated 15.6 years ago • Tim Rayner

results = res, sep = sep, file = paste(c(x[[1]]$bg, x[[1]]$norm, "lmfit.csv"), collapse = "")) write.fit(fit2, results = res, sep = sep, file = paste(c(x[[1]]$bg, x[[1]]$norm, "be.csv"), collapse = "")) where x[[1]]$avg is the corrected

Annotation limma Annotation limma

updated 12.8 years ago • Cornish, Joseph NIH/NIAID [F]

254 61 80 79 876 571218 131 42 61 47 246 > Norm<-normalize.quantiles(BG) > dim(Norm) [1] 473162 15 > Norm[1:10,1:5] [,1] [,2] [,3] [,4] [,5] [1,] 128.4667 143.40000 114.06667 332.7333 230.0000...195.80000 482.5333 602.5667 [10,] 125.7333 89.73333 131.33333 103.0667 187.9333 &…

Preprocessing Preprocessing

updated 17.5 years ago • Ann Hess

FALSE, quantile=0.9, groups =files$Group, verbose=TRUE) s.norm <- normalizeCtData(raw.cat, norm="scale.rank") exprs(s.norm) write.table(exprs(s.norm),file="Ct norm scaling.txt") g.norm <- normalizeCtData(raw.cat, norm...geometric.mean") exprs(g.norm) write.table(exprs(g.norm),file="Ct norm media geometrica.txt") Now if we look at the content of the two expression value files, it …

Normalization HTqPCR Normalization HTqPCR

updated 12.4 years ago • Guest User

access the slot 'count'. After fixing that, it runs into: Error in qpHexbin(mnorm, main = "MA-Plot :: Norm") : couldn't find function "hexagons" Error in qpHexbin(mnorm, main = "MA-Plot :: Norm") : couldn't find function "hex.legend" Which I also

hexbin arrayQuality hexbin arrayQuality

updated 20.6 years ago • Paquet, Agnes

of memory. apt-cel-extract -o out.txt [-c chip.clf -p chip.pgf] [-d chip.cdf] \ --probeset-ids=norm-exon.txt --probeset-ids=norm-intron.txt \ --probe-ids=antigenomic.bgp *.cel Hope it helps Raffaele shirley zhang wrote: &gt

probe probe

updated 17.8 years ago • raffaele calogero

work for my data set and I am getting the following error message: > dat.Norm = maNorm(dat.RAW, norm="p") Error in simpleLoess(y, x, w, span, degree, parametric, drop.square, normalize, : invalid `x' Is there any way to use maNorm() successfully

updated 22.0 years ago • Mahbub Latif

against a single normalization control. Below is the code and the error. <pre> > NORM <- "hsa-miR-30a-5p" > idx <- which( rownames( Counts.deseq )==NORM ) > Counts.deseq.cnorm <- estimateSizeFactors( Counts.deseq

deseq2 bug

updated 10.9 years ago • Johannes Rainer

nbsp; Here is my code: <pre> eset57338 <- (getDataset("GSE57338", "GPL11532",format = "CURESET", norm = "SCAN", features = "GENE")) eset42955 <- (getDataset("GSE42955", "GPL6244", format = "CURESET",norm = "SCAN", features = "GENE")) thelist <- list

limma eset error ebayes

updated 10.5 years ago • Vani

Hi! I have some questions in eliminating efficiency after reading the csaw user's guide. In the examples of the user's guide, two of them filter windows and then use TMM for normalization to eliminate composition biaes. However, in [5.3 Eliminating efficiency biases](http://bioconductor.org/books/3.15/csawBook/chap-norm.html#sec:eff-norm), windows are also filtered first and then applied to TMM…

csawUsersGuide csaw

updated 2.9 years ago • sashu