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10x
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Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
10 months ago
rocanja
▴ 60
0
votes
1
reply
1.0k
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 21 months ago by
Peter Hickey
▴ 750 • written 21 months ago by
sandra.garces.19
• 0
0
votes
2
replies
953
views
Compare and filter three BAM files
RNAseq
10X
22 months ago
wt215
• 0
4
votes
5
replies
3.4k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
2.5 years ago
Peter Hickey
▴ 750
1
vote
1
reply
1.3k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 4.0 years ago by
Aaron Lun
★ 28k • written 4.0 years ago by
Peter Hickey
▴ 750
5
votes
3
replies
1.7k
views
Latent factors for differential expression
deseq2
zinbwave
10X
scRNA-seq
Differential expression
updated 4.6 years ago by
Michael Love
43k • written 4.6 years ago by
vincent.croset
▴ 20
4
votes
2
replies
3.1k
views
When to combine samples in the pre-processing of 10x scRNA-seq data?
10x
scrna-seq
updated 5.8 years ago by
Aaron Lun
★ 28k • written 5.8 years ago by
jma1991
▴ 70
7
votes
12
replies
8.1k
views
How to identify real cells in 10X RNA-seq ?
single-cell
10X
Cell calling
DropletUtils
cellranger
updated 5.9 years ago by
Aaron Lun
★ 28k • written 5.9 years ago by
xingxd16
▴ 20
8 results • Page
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Comment: displaying the differential expression with deseq2 over 4X2 samples with the in
by
Martijn
• 0
thanks James, but if I do pairwise analyses per subject, I get the impression that I only get the intercepts per gene. Do you mean regardin…
Comment: plyranges: find exact GRanges match keeping metadata columns
by
alexis.weinreb
• 0
Thank you, this does clarify the available options.
Answer: displaying the differential expression with deseq2 over 4X2 samples with the in
by
James W. MacDonald
67k
If you have paired data you should block on subject, which is algebraically the same as fitting a model on the difference between treated a…
Answer: How to compare expression levels of genes between scRNAseq and bulkRNAseq?
by
jodan
• 0
Your approach sounds solid! You might also consider plotting a scatter plot comparing log2 fold changes from both datasets to identify gene…
Answer: Unused arguments error without used arguments in GSVA?
by
jodan
• 0
It looks like the ssgseaParam function might not be passing the correct parameters to GSVA::gsva(). The error suggests that minSize and max…
Votes
Answer: Find exact GRanges match keeping metadata columns
Answer: Find exact GRanges match keeping metadata columns
Answer: Unused arguments error without used arguments in GSVA?
Answer: Unused arguments error without used arguments in GSVA?
Answer: Unused arguments error without used arguments in GSVA?
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