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emptyDrops new default alpha = Inf giving far fewer non-empty droplets than under old default of alpha = NULL
scrn
10x
scRNAseq
emptyDrops
DropletUtils
5 months ago • updated 4 months ago
Peter Hickey
▴ 760
1
vote
1
reply
1.7k
views
Origin of the labels for the SingleCellMultiModal dataset
labels
PBMC
SingleCellMultiModal
10X
updated 9 months ago by
ATpoint
★ 5.0k • written 9 months ago by
javier.ruizramirez
• 0
0
votes
2
replies
1.1k
views
Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
21 months ago
rocanja
▴ 60
0
votes
1
reply
1.5k
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 2.7 years ago by
Peter Hickey
▴ 760 • written 2.7 years ago by
sandra.garces.19
• 0
0
votes
2
replies
1.3k
views
Compare and filter three BAM files
RNAseq
10X
2.8 years ago
wt215
• 0
4
votes
5
replies
4.5k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
3.4 years ago
Peter Hickey
▴ 760
1
vote
1
reply
1.6k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 5.0 years ago by
Aaron Lun
★ 29k • written 5.0 years ago by
Peter Hickey
▴ 760
5
votes
3
replies
2.2k
views
Latent factors for differential expression
deseq2
zinbwave
10X
scRNA-seq
Differential expression
updated 5.5 years ago by
Michael Love
43k • written 5.5 years ago by
vincent.croset
▴ 20
4
votes
2
replies
3.8k
views
When to combine samples in the pre-processing of 10x scRNA-seq data?
10x
scrna-seq
updated 6.7 years ago by
Aaron Lun
★ 29k • written 6.7 years ago by
jma1991
▴ 70
7
votes
12
replies
9.7k
views
How to identify real cells in 10X RNA-seq ?
single-cell
10X
Cell calling
DropletUtils
cellranger
updated 6.8 years ago by
Aaron Lun
★ 29k • written 6.8 years ago by
xingxd16
▴ 20
10 results • Page
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Comment: Guitar R package requires update due to dependency GenomicFeatures
by
ferran.llobet.cabau
▴ 10
Thank you @seandavi for your feedback. I followed your recommendations and generated a [pull request][1] including the fix for this issue…
Comment: Ensembl plants at annotationHub?
by
Guido Hooiveld
★ 4.1k
Hope you don't mind I am not Johannes... ;-) Note that you nowadays can generate these yourselves using the `docker` image Johannes made av…
Comment: How does contrasts.fit do in limma to extract comparisons with a model having in
by
jtsy6383
• 0
Thanks for the reminder about DiffVar (for that project I ended up using GAMLSS in the end and haven't touched DiffVar since) and the alter…
Comment: Ensembl plants at annotationHub?
by
jwp48
• 0
Dear Johannes, Would it be possible to generate a EnsDb for the newest Medicago V5 genome release? Medicago truncatula A17 r5.0 This woul…
Answer: please provide a BibTeX citation for "Advanced Single-Cell Analysis with Biocond
by
Ludwig Geistlinger
▴ 80
When referring to the OSCA book including individual sub-books please cite the OSCA paper: Amezquita, R.A., Lun, A.T.L., Becht, E. et a…
Votes
limma moderated t-statistics and B-statistics
Comment: Guitar R package requires update due to dependency GenomicFeatures
A: how to control age and gender effect in expression data using DESeq2
A: how to control age and gender effect in expression data using DESeq2
how to control age and gender effect in expression data using DESeq2
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