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10x
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11
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emptyDrops new default alpha = Inf giving far fewer non-empty droplets than under old default of alpha = NULL
scrn
10x
scRNAseq
emptyDrops
DropletUtils
10 weeks ago • updated 9 weeks ago
Peter Hickey
▴ 760
1
vote
1
reply
1.6k
views
Origin of the labels for the SingleCellMultiModal dataset
labels
PBMC
SingleCellMultiModal
10X
updated 6 months ago by
ATpoint
★ 5.0k • written 6 months ago by
javier.ruizramirez
• 0
0
votes
2
replies
1.0k
views
Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
19 months ago
rocanja
▴ 60
0
votes
1
reply
1.4k
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 2.4 years ago by
Peter Hickey
▴ 760 • written 2.5 years ago by
sandra.garces.19
• 0
0
votes
2
replies
1.2k
views
Compare and filter three BAM files
RNAseq
10X
2.6 years ago
wt215
• 0
4
votes
5
replies
4.1k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
3.2 years ago
Peter Hickey
▴ 760
1
vote
1
reply
1.5k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 4.7 years ago by
Aaron Lun
★ 29k • written 4.7 years ago by
Peter Hickey
▴ 760
5
votes
3
replies
2.1k
views
Latent factors for differential expression
deseq2
zinbwave
10X
scRNA-seq
Differential expression
updated 5.3 years ago by
Michael Love
43k • written 5.3 years ago by
vincent.croset
▴ 20
4
votes
2
replies
3.7k
views
When to combine samples in the pre-processing of 10x scRNA-seq data?
10x
scrna-seq
updated 6.5 years ago by
Aaron Lun
★ 29k • written 6.5 years ago by
jma1991
▴ 70
7
votes
12
replies
9.2k
views
How to identify real cells in 10X RNA-seq ?
single-cell
10X
Cell calling
DropletUtils
cellranger
updated 6.6 years ago by
Aaron Lun
★ 29k • written 6.6 years ago by
xingxd16
▴ 20
10 results • Page
1 of 1
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Comment: Best practice for handling large data (matrix with >2^31-1 non-zero elements) in
by
dan.gatti
• 0
Was anyone else able to put the pieces together using the reply above? I read in one 10X sample using DropletUtils::read10xCounts() and thi…
Comment: Announcing GBCC 2025: The first-ever joint conference between Bioconductor and G
by
dzv20413
• 0
Wow, GBCC 2025 sounds amazing, can not wait to see the logo contest results! Also, I just played [Level Devil][1] and it is super addictive…
Comment: Memory error while running alignments
by
Erik Wright
▴ 150
How much memory is available on the computer? A distance matrix of 50k sequences requires at least 50k*50k/2 * 8 bytes = 10GB, assuming the…
Comment: NA values for DESeq2 p-values and adjusted p-values using large sample sizes
by
Gabriel
• 0
Thank you very much for the reply. However, none of these three options seems to fit: 1) The genes for which NA appears do not have all 0 …
Comment: "organism" missing in BSgenome.Hsapiens.UCSC.hg38 slotNames
by
ubjw24
• 0
Thank you for your replies. I got the problem solved by installing mutSignature with this line: ``` devtools::install_github("dami82/mutS…
Votes
Comment: Check removeBatchEffect effectiveness
Comment: Streamlining the computing time for MiloDE p-value correction in large dataset?
Answer: DESeq2 design for haplotype MPRA
A: Different logFC (log2foldchange) values for genes from limma-voom and other too
Comment: Check removeBatchEffect effectiveness
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