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emptyDrops new default alpha = Inf giving far fewer non-empty droplets than under old default of alpha = NULL
scrn
10x
scRNAseq
emptyDrops
DropletUtils
3 months ago
Peter Hickey
▴ 760
1
vote
1
reply
1.7k
views
Origin of the labels for the SingleCellMultiModal dataset
labels
PBMC
SingleCellMultiModal
10X
updated 7 months ago by
ATpoint
★ 5.0k • written 7 months ago by
javier.ruizramirez
• 0
0
votes
2
replies
1.1k
views
Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
20 months ago
rocanja
▴ 60
0
votes
1
reply
1.5k
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 2.6 years ago by
Peter Hickey
▴ 760 • written 2.6 years ago by
sandra.garces.19
• 0
0
votes
2
replies
1.2k
views
Compare and filter three BAM files
RNAseq
10X
2.7 years ago
wt215
• 0
4
votes
5
replies
4.2k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
3.3 years ago
Peter Hickey
▴ 760
1
vote
1
reply
1.5k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 4.9 years ago by
Aaron Lun
★ 29k • written 4.9 years ago by
Peter Hickey
▴ 760
5
votes
3
replies
2.2k
views
Latent factors for differential expression
deseq2
zinbwave
10X
scRNA-seq
Differential expression
updated 5.4 years ago by
Michael Love
43k • written 5.4 years ago by
vincent.croset
▴ 20
4
votes
2
replies
3.8k
views
When to combine samples in the pre-processing of 10x scRNA-seq data?
10x
scrna-seq
updated 6.6 years ago by
Aaron Lun
★ 29k • written 6.6 years ago by
jma1991
▴ 70
7
votes
12
replies
9.3k
views
How to identify real cells in 10X RNA-seq ?
single-cell
10X
Cell calling
DropletUtils
cellranger
updated 6.7 years ago by
Aaron Lun
★ 29k • written 6.7 years ago by
xingxd16
▴ 20
10 results • Page
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Comment: Question about DESeq2 Design for Paired Treatment Study
by
ATpoint
★ 5.0k
It's a paired analysis so you don't need any patient-specific covariates such as age and sex. What you call batch effect might just be the …
Comment: problem reading bam files into nucleR
by
efoss
▴ 10
I thought I might be able to track down a problematic line in the bam file by repeatedly splitting the bam file in and then running the co…
Answer: two questions about limma
by
Gordon Smyth
53k
Answered here: https://support.bioconductor.org/p/16318/
Comment: Highly similar RNA-seq samples in PCA - pooling or technical duplication?
by
Ahmed Salah
• 0
Thank you very much! will take that into consideration
Comment: Large logFC but somewhat high FDR
by
JKim
• 0
Thank you very much for taking the time to answer the question. I really appreciate your insights and comments.
Votes
Comment: Highly similar RNA-seq samples in PCA - pooling or technical duplication?
Answer: Large logFC but somewhat high FDR
Comment: upstream git branches of bioc-release
Comment: upstream git branches of bioc-release
Comment: upstream git branches of bioc-release
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