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EmptyDrops
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0
votes
2
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800
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Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
13 months ago
rocanja
▴ 60
0
votes
1
reply
1.2k
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 23 months ago by
Peter Hickey
▴ 750 • written 24 months ago by
sandra.garces.19
• 0
4
votes
5
replies
3.7k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
2.7 years ago
Peter Hickey
▴ 750
1
vote
1
reply
1.3k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 4.3 years ago by
Aaron Lun
★ 28k • written 4.3 years ago by
Peter Hickey
▴ 750
4
votes
5
replies
2.6k
views
emptyDrops identified less non-empty cells with lower total UMI counts?
DropletUtils
scRNA-seq
emptyDrops
simplesinglecell
cell calling
updated 4.5 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
C T
▴ 140
5
votes
1
reply
5.2k
views
Error with DropletUtils::EmptyDrops()
DropletUtils
EmptyDrops
simpleSingleCell
updated 2.7 years ago by
Stefano Mangiola
▴ 40 • written 5.7 years ago by
shbrief
▴ 20
0
votes
2
replies
1.4k
views
Scalability of emptyDrops() running times
dropletutils
emptydrops
updated 6.0 years ago by
Aaron Lun
★ 28k • written 6.0 years ago by
rosano
• 0
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Comment: Bioconductor 3.20 Release Schedule
by
randyprice13102000
• 0
This offers much info https://bioconductor.org/checkResults/release/bioc-LATEST/
Comment: Filtering after DESeq
by
ATpoint
★ 4.8k
The problem is that your prefilter is not group-aware so the behaviour of seeing these sorts of outliers is expected. This is why I always …
Comment: GSEA preranked analysis downstream of DEseq2
by
ATpoint
★ 4.8k
All due respect, this is what people do since pvalues exist, so this paper is not a reference, not is this a special score that merits a na…
Comment: Check removeBatchEffect effectiveness
by
ATpoint
★ 4.8k
> insert size, mean GC, etc... This is not a classical "batch" in the common sense, at least I've never seen this to be corrected for expl…
Answer: Check removeBatchEffect effectiveness
by
Gordon Smyth
52k
Your call to removeBatchEffect() is not correct because that function does not have an argument called `batch3`, meaning that the batch fac…
Votes
Answer: DiffBind; Error: No sites have activity greater than filter value
DiffBind; Error: No sites have activity greater than filter value
remove X and Y chromosome genes in RNA-seq data using DESeq2 pipeline
How to remove X & Y chromosome genes from RNAseq data
Is it advisable to remove X and Y chromosome genes in mouse bulk RNA-seq data at the level of the count matrix?
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