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simpleSingleCell
•
reset
4
votes
5
replies
2.3k
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emptyDrops identified less non-empty cells with lower total UMI counts?
DropletUtils
scRNA-seq
emptyDrops
simplesinglecell
cell calling
updated 4.1 years ago by
Aaron Lun
★ 28k • written 5.3 years ago by
C T
▴ 140
4
votes
6
replies
1.5k
views
Feature selection across batches in simpleSingleCell when spike-ins are not available.
simpleSingleCell
updated 5.3 years ago by
Aaron Lun
★ 28k • written 5.3 years ago by
Angelos Armen
• 0
1
vote
1
reply
833
views
Feature selection across batches in simpleSingleCell "Correcting batch effects" vignette
simpleSingleCell
updated 5.3 years ago by
Aaron Lun
★ 28k • written 5.3 years ago by
Angelos Armen
• 0
5
votes
1
reply
5.0k
views
Error with DropletUtils::EmptyDrops()
DropletUtils
EmptyDrops
simpleSingleCell
updated 2.3 years ago by
Stefano Mangiola
▴ 20 • written 5.3 years ago by
shbrief
▴ 20
1
vote
5
replies
1.9k
views
Preprocessing in scran::doubletCells
scran
simpleSingleCell
updated 5.4 years ago by
Aaron Lun
★ 28k • written 5.4 years ago by
Angelos Armen
• 0
4
votes
4
replies
3.1k
views
using limma and edgeR for differential expression of Drop-seq/10xGenomics scRNA-seq data
limma
edgeR
simplesingleCell
scran
scater
updated 5.4 years ago by
Aaron Lun
★ 28k • written 5.4 years ago by
Bogdan
▴ 670
4
votes
2
replies
1.9k
views
single cell data: adding random effects for subject with multilevel experiment design
SingleCellExperiment
scran
limma
simpleSingleCell
updated 5.5 years ago by
Aaron Lun
★ 28k • written 5.5 years ago by
Matt Mulé
• 0
1
vote
3
replies
1.9k
views
Troubles with barcodeRanks()
DropletUtils
SingleCellExperiement
DelayedArray
simpleSingleCell
updated 5.5 years ago by
Aaron Lun
★ 28k • written 5.5 years ago by
Steve Pederson
▴ 170
8
votes
2
replies
2.9k
views
Recommendations for combining multiple 10x runs into one SingleCellExperiment
singlecellexperiment
dropletutils
simplesinglecell
updated 6.1 years ago by
Aaron Lun
★ 28k • written 6.2 years ago by
Peter Hickey
▴ 740
0
votes
1
reply
1.0k
views
Unusual graph for Identifying HVGs from the normalized log-expression
workflows
simplesinglecell
updated 6.2 years ago by
Aaron Lun
★ 28k • written 6.2 years ago by
es.azadian
• 0
0
votes
1
reply
1.1k
views
The explanation of plotExplanatoryVariables plot
simplesinglecell
updated 6.7 years ago by
Aaron Lun
★ 28k • written 6.7 years ago by
Xingyu
• 0
1
vote
2
replies
2.0k
views
SingleCellExperiment object cannot be used by Scater package?
scater
singlecellexperiment
simplesinglecell
updated 6.8 years ago by
Aaron Lun
★ 28k • written 6.8 years ago by
Xingyu
• 0
3
votes
2
replies
3.6k
views
Sparse matrices in Bioconductor objects for single-cell analyses
scater
simpleSingleCell
workflows
updated 7.5 years ago by
Aaron Lun
★ 28k • written 7.5 years ago by
sandmann.t
▴ 70
13 results • Page
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Comment: Running multiple KNN queries in parallel
by
SamGG
▴ 360
Thank you for your detailed answer, I appreciate it and your work.
Answer: Running multiple KNN queries in parallel
by
Aaron Lun
★ 28k
That is correct - you can't pass the prebuilt objects to different processes. Maybe `MulticoreParam()` might work on POSIX systems but I'm …
Answer: RSubread output significantly different from bash Subread output
by
Gordon Smyth
52k
The difference (as ATpoint has correctly pointed out) is that Rsubread::featureCounts counts multi-mapping reads by default whereas the com…
Answer: Deseq2 Error in solve.default(qr.R(qrx)) : 'a' (1 x 0) must be square
by
Michael Love
43k
I would check the rank of your model matrix, and perhaps meet with a local statistician who can help you create an appropriate design matri…
Comment: sva: get a sv for RIN scores
by
gabriel.hoffman
▴ 170
Agreed. I'll add that with small sample size, a covariate can explain some fraction of expression variation even under the null.
Votes
Answer: Running multiple KNN queries in parallel
Answer: Running multiple KNN queries in parallel
Answer: RSubread output significantly different from bash Subread output
Answer: RSubread output significantly different from bash Subread output
Answer: How to annotate a limma Elist raw class object with an annotation file
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ATpoint
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Gordon Smyth
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