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RNA Seq
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0
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9
replies
2.6k
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How to create manhattan plot with DESeq2
deseq2
mahattan plot
RNA Seq
updated 3.6 years ago by
bernatgel
▴ 150 • written 4.5 years ago by
adeler001
• 0
1
vote
5
replies
1.3k
views
Time course experiment using limma with voom
limma
voom
RNA Seq
Time-course experiments
updated 4.9 years ago by
Aaron Lun
★ 28k • written 4.9 years ago by
mahes.muniandy
• 0
2
votes
5
replies
3.2k
views
Possible batch effect correction with RNA-Seq data when the batches do not contain both biological conditions for DE analysis
batch effect
limma
rna seq
DE
updated 4.0 years ago by
Steve Lianoglou
★ 13k • written 4.0 years ago by
svlachavas
▴ 830
1
vote
4
replies
893
views
Difference between Fold Changes from EdgeR glmLRTest and directly calculated fold changes.
edger
RNA Seq
updated 4.4 years ago by
James W. MacDonald
65k • written 4.4 years ago by
abf
▴ 30
3
votes
3
replies
885
views
Custom gene annotations for SGSeq
sgseq
alternative splicing
rna seq
updated 4.4 years ago by
Leonard Goldstein
▴ 200 • written 4.4 years ago by
116100016
• 0
5
votes
3
replies
962
views
Steep dendrograms and few modules. WGCNA on RNA seq data
RNA seq
wgcna
updated 4.9 years ago by
Peter Langfelder
★ 3.0k • written 4.9 years ago by
agustin.gonvi
▴ 20
0
votes
3
replies
650
views
Normalization of RNA seq data
RNA seq
cpm
updated 4.1 years ago by
Kevin Blighe
★ 3.9k • written 4.1 years ago by
isabel.pieterse
• 0
0
votes
2
replies
777
views
using only uniquely-mapping reads for alternative splicing using SGSeq
rna seq
SGSeq
Alternative splicing
updated 4.6 years ago by
Leonard Goldstein
▴ 200 • written 4.6 years ago by
116100016
• 0
1
vote
2
replies
1.9k
views
adapter trimming paired vs unpaired reads
rna seq
trimmomatic
trimming
adapter trimming
paired analysis
5.0 years ago
Mozart
▴ 30
0
votes
2
replies
947
views
Need help cprrecting overlappinge genes on valcano plot
volcano plot
rna seq
updated 4.7 years ago by
Michael Love
41k • written 4.7 years ago by
adeler001
• 0
0
votes
1
reply
550
views
51 views DESeq2 RNAseq differential expression analysis
deseq2
likelihood ratio
rna seq
wald test
updated 4.6 years ago by
Michael Love
41k • written 4.6 years ago by
adeler001
• 0
0
votes
0
replies
497
views
searching for specific RNA sequences
rna seq
4.5 years ago
adeler001
• 0
0
votes
0
replies
1.6k
views
Sub clustering from hierarchical cluster used to make heat map of gene expression data
heatmap.2
hierarchical clustering
microarray
RNA seq
DEG
6.2 years ago
lawarde.ankita1
▴ 30
13 results • Page
1 of 1
Recent ...
Replies
Answer: Once again a "Model matrix not full rank"
by
swbarnes2
★ 1.3k
Replicate numbers, like the 1 in control_1 are fine in sample names, but never add them to anything else in colData. From the computer's p…
Answer: limma Intercept vs No-intercept models completely changing DMR results?
by
Gordon Smyth
50k
I'll add a little bit of general advice to James' answer. You seem to have the misunderstanding that you can change the design matrix but …
Answer: package goseq seems to be not available on the latest version of R
by
Gordon Smyth
50k
It is true that goseq isn't available for Bioc 3.19. That is because goseq depends on txbmaker, which is itself not yet available for Bioc…
Answer: limma Intercept vs No-intercept models completely changing DMR results?
by
James W. MacDonald
65k
This part: ``` design_intercept <- model.matrix(~Alt+Sex+Age+PC1, data=targets_Sherpa) colnames(design_intercept) <- c("AltSHP_LA","Al…
Comment: CombineArrays for EPIC and EPIC V2
by
Kim
• 0
Thank you Tim, this is a great help in getting me started!
Votes
Answer: limma Intercept vs No-intercept models completely changing DMR results?
Answer: CombineArrays for EPIC and EPIC V2
Answer: Too many significant genes when integrating gtex and tcga
Comment: Too many significant genes when integrating gtex and tcga
A: Print Differentially Expressed Exons From Dexseq Results
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