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RNA seq
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0
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9
replies
2.6k
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How to create manhattan plot with DESeq2
deseq2
mahattan plot
RNA Seq
updated 3.5 years ago by
bernatgel
▴ 150 • written 4.5 years ago by
adeler001
• 0
2
votes
5
replies
3.2k
views
Possible batch effect correction with RNA-Seq data when the batches do not contain both biological conditions for DE analysis
batch effect
limma
rna seq
DE
updated 4.0 years ago by
Steve Lianoglou
★ 13k • written 4.0 years ago by
svlachavas
▴ 830
0
votes
3
replies
641
views
Normalization of RNA seq data
RNA seq
cpm
updated 4.0 years ago by
Kevin Blighe
★ 3.9k • written 4.0 years ago by
isabel.pieterse
• 0
3
votes
3
replies
867
views
Custom gene annotations for SGSeq
sgseq
alternative splicing
rna seq
updated 4.4 years ago by
Leonard Goldstein
▴ 170 • written 4.4 years ago by
116100016
• 0
1
vote
4
replies
884
views
Difference between Fold Changes from EdgeR glmLRTest and directly calculated fold changes.
edger
RNA Seq
updated 4.4 years ago by
James W. MacDonald
65k • written 4.4 years ago by
abf
▴ 30
0
votes
0
replies
485
views
searching for specific RNA sequences
rna seq
4.4 years ago
adeler001
• 0
0
votes
2
replies
764
views
using only uniquely-mapping reads for alternative splicing using SGSeq
rna seq
SGSeq
Alternative splicing
updated 4.5 years ago by
Leonard Goldstein
▴ 170 • written 4.5 years ago by
116100016
• 0
0
votes
1
reply
545
views
51 views DESeq2 RNAseq differential expression analysis
deseq2
likelihood ratio
rna seq
wald test
updated 4.6 years ago by
Michael Love
41k • written 4.6 years ago by
adeler001
• 0
0
votes
2
replies
934
views
Need help cprrecting overlappinge genes on valcano plot
volcano plot
rna seq
updated 4.6 years ago by
Michael Love
41k • written 4.6 years ago by
adeler001
• 0
1
vote
5
replies
1.2k
views
Time course experiment using limma with voom
limma
voom
RNA Seq
Time-course experiments
updated 4.8 years ago by
Aaron Lun
★ 28k • written 4.8 years ago by
mahes.muniandy
• 0
5
votes
3
replies
935
views
Steep dendrograms and few modules. WGCNA on RNA seq data
RNA seq
wgcna
updated 4.9 years ago by
Peter Langfelder
★ 3.0k • written 4.9 years ago by
agustin.gonvi
▴ 20
1
vote
2
replies
1.9k
views
adapter trimming paired vs unpaired reads
rna seq
trimmomatic
trimming
adapter trimming
paired analysis
5.0 years ago
Mozart
▴ 30
0
votes
0
replies
1.6k
views
Sub clustering from hierarchical cluster used to make heat map of gene expression data
heatmap.2
hierarchical clustering
microarray
RNA seq
DEG
6.2 years ago
lawarde.ankita1
▴ 30
13 results • Page
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Comment: The difference when using Limma compare between 3 conditions vs 2 conditions
by
Chris
• 0
Thanks Robert!
Comment: The difference when using Limma compare between 3 conditions vs 2 conditions
by
Robert Castelo
★ 3.2k
Hi, your `c2` object storing all the gene sets is probably a `list` object, where element names are the names of the pathways given by MSig…
Comment: Differential peaks that were not identified in diffbind were identified in diseq
by
lonn
• 0
I tried the default normalization and got similar results as above. Perhaps I need to try identifying the difference peak without using dif…
Answer: Cannabis and KEGGgraph
by
deeenes
▴ 30
Hello, I'm a developer of the `OmnipathR` package, and I wrote [a function][1] to access certain KEGG data: library(OmnipathR) cs…
Answer: RNAseq:Technical replicates from different batches
by
Gordon Smyth
50k
I am not entirely clear what you mean by "technical" replicates in this case. Are they resequencing of the same RNA samples or are they fre…
Votes
Comment: The difference when using Limma compare between 3 conditions vs 2 conditions
Installing/switching between versions of R/Rstudio/Bioconductor on personal machine (Linux | Ubuntu)
A: Docker container with bioconductor and devtools
Answer: OrgDb for maize
Comment: significant difference of p-values with dream() after updating VariancePartition
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