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simplesinglecell
•
reset
4
votes
5
replies
3.1k
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emptyDrops identified less non-empty cells with lower total UMI counts?
DropletUtils
scRNA-seq
emptyDrops
simplesinglecell
cell calling
updated 5.2 years ago by
Aaron Lun
★ 29k • written 6.4 years ago by
C T
▴ 140
4
votes
6
replies
1.9k
views
Feature selection across batches in simpleSingleCell when spike-ins are not available.
simpleSingleCell
updated 6.4 years ago by
Aaron Lun
★ 29k • written 6.4 years ago by
Angelos Armen
• 0
1
vote
1
reply
1.1k
views
Feature selection across batches in simpleSingleCell "Correcting batch effects" vignette
simpleSingleCell
updated 6.4 years ago by
Aaron Lun
★ 29k • written 6.4 years ago by
Angelos Armen
• 0
5
votes
1
reply
5.6k
views
Error with DropletUtils::EmptyDrops()
DropletUtils
EmptyDrops
simpleSingleCell
updated 3.4 years ago by
Stefano Mangiola
▴ 40 • written 6.4 years ago by
shbrief
▴ 20
1
vote
5
replies
2.5k
views
Preprocessing in scran::doubletCells
scran
simpleSingleCell
updated 6.5 years ago by
Aaron Lun
★ 29k • written 6.5 years ago by
Angelos Armen
• 0
4
votes
4
replies
3.8k
views
using limma and edgeR for differential expression of Drop-seq/10xGenomics scRNA-seq data
limma
edgeR
simplesingleCell
scran
scater
updated 6.5 years ago by
Aaron Lun
★ 29k • written 6.5 years ago by
Bogdan
▴ 670
4
votes
2
replies
2.4k
views
single cell data: adding random effects for subject with multilevel experiment design
SingleCellExperiment
scran
limma
simpleSingleCell
updated 6.6 years ago by
Aaron Lun
★ 29k • written 6.6 years ago by
Matt Mulé
• 0
1
vote
3
replies
2.3k
views
Troubles with barcodeRanks()
DropletUtils
SingleCellExperiement
DelayedArray
simpleSingleCell
updated 6.6 years ago by
Aaron Lun
★ 29k • written 6.6 years ago by
Steve Pederson
▴ 170
8
votes
2
replies
3.8k
views
Recommendations for combining multiple 10x runs into one SingleCellExperiment
singlecellexperiment
dropletutils
simplesinglecell
updated 7.2 years ago by
Aaron Lun
★ 29k • written 7.2 years ago by
Peter Hickey
▴ 760
0
votes
1
reply
1.3k
views
Unusual graph for Identifying HVGs from the normalized log-expression
workflows
simplesinglecell
updated 7.3 years ago by
Aaron Lun
★ 29k • written 7.3 years ago by
es.azadian
• 0
0
votes
1
reply
1.4k
views
The explanation of plotExplanatoryVariables plot
simplesinglecell
updated 7.8 years ago by
Aaron Lun
★ 29k • written 7.8 years ago by
Xingyu
• 0
1
vote
2
replies
2.4k
views
SingleCellExperiment object cannot be used by Scater package?
scater
singlecellexperiment
simplesinglecell
updated 7.9 years ago by
Aaron Lun
★ 29k • written 7.9 years ago by
Xingyu
• 0
3
votes
2
replies
4.3k
views
Sparse matrices in Bioconductor objects for single-cell analyses
scater
simpleSingleCell
workflows
updated 8.6 years ago by
Aaron Lun
★ 29k • written 8.6 years ago by
sandmann.t
▴ 70
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Comment: Incosistencies in buildSNNGraph() function
by
ATpoint
★ 5.0k
Just played with this a bit and I think it comes down to not having a fixed seed before running the leiden thingy. Same goes for PCA and UM…
Answer: Incosistencies in buildSNNGraph() function
by
Aaron Lun
★ 29k
The most obvious thing that comes to mind is the RNG seed. `cluster_leiden()` does some random number generation, somewhere deep in the **i…
Comment: Opportunity to host EuroBioC2026 Preconference workshops!
by
TikTsokaas33ocissalpc
• 0
Thank you for sharing the EuroBioC2026 workshop opportunity. I m interested in hosting a hands on session teaching reproducible data analys…
Comment: Ensembl plants at annotationHub?
by
jwp48
• 0
Nope I don't mind haha! Anyway thanks so much! Thats super kind of you!
Answer: Combining two proteomics datasets with limpa
by
Gordon Smyth
53k
Hi Andrew, We don't have that much experience combining different datasets with limpa yet, but my feeling is that the same DPC should be u…
Votes
Answer: Incosistencies in buildSNNGraph() function
Answer: Where do the filename come from in the LOLA datasets?
Bioconductor Maintainer Application
A: What is logFC and "B" in methylation analysis ??
limma moderated t-statistics and B-statistics
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