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simplesinglecell
•
reset
4
votes
5
replies
2.5k
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emptyDrops identified less non-empty cells with lower total UMI counts?
DropletUtils
scRNA-seq
emptyDrops
simplesinglecell
cell calling
updated 4.4 years ago by
Aaron Lun
★ 28k • written 5.6 years ago by
C T
▴ 140
4
votes
6
replies
1.6k
views
Feature selection across batches in simpleSingleCell when spike-ins are not available.
simpleSingleCell
updated 5.6 years ago by
Aaron Lun
★ 28k • written 5.6 years ago by
Angelos Armen
• 0
1
vote
1
reply
889
views
Feature selection across batches in simpleSingleCell "Correcting batch effects" vignette
simpleSingleCell
updated 5.6 years ago by
Aaron Lun
★ 28k • written 5.6 years ago by
Angelos Armen
• 0
5
votes
1
reply
5.2k
views
Error with DropletUtils::EmptyDrops()
DropletUtils
EmptyDrops
simpleSingleCell
updated 2.6 years ago by
Stefano Mangiola
▴ 40 • written 5.6 years ago by
shbrief
▴ 20
1
vote
5
replies
2.1k
views
Preprocessing in scran::doubletCells
scran
simpleSingleCell
updated 5.7 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
Angelos Armen
• 0
4
votes
4
replies
3.3k
views
using limma and edgeR for differential expression of Drop-seq/10xGenomics scRNA-seq data
limma
edgeR
simplesingleCell
scran
scater
updated 5.7 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
Bogdan
▴ 670
4
votes
2
replies
2.1k
views
single cell data: adding random effects for subject with multilevel experiment design
SingleCellExperiment
scran
limma
simpleSingleCell
updated 5.8 years ago by
Aaron Lun
★ 28k • written 5.8 years ago by
Matt Mulé
• 0
1
vote
3
replies
2.0k
views
Troubles with barcodeRanks()
DropletUtils
SingleCellExperiement
DelayedArray
simpleSingleCell
updated 5.8 years ago by
Aaron Lun
★ 28k • written 5.8 years ago by
Steve Pederson
▴ 170
8
votes
2
replies
3.2k
views
Recommendations for combining multiple 10x runs into one SingleCellExperiment
singlecellexperiment
dropletutils
simplesinglecell
updated 6.4 years ago by
Aaron Lun
★ 28k • written 6.4 years ago by
Peter Hickey
▴ 750
0
votes
1
reply
1.1k
views
Unusual graph for Identifying HVGs from the normalized log-expression
workflows
simplesinglecell
updated 6.5 years ago by
Aaron Lun
★ 28k • written 6.5 years ago by
es.azadian
• 0
0
votes
1
reply
1.1k
views
The explanation of plotExplanatoryVariables plot
simplesinglecell
updated 7.0 years ago by
Aaron Lun
★ 28k • written 7.0 years ago by
Xingyu
• 0
1
vote
2
replies
2.1k
views
SingleCellExperiment object cannot be used by Scater package?
scater
singlecellexperiment
simplesinglecell
updated 7.1 years ago by
Aaron Lun
★ 28k • written 7.1 years ago by
Xingyu
• 0
3
votes
2
replies
3.8k
views
Sparse matrices in Bioconductor objects for single-cell analyses
scater
simpleSingleCell
workflows
updated 7.8 years ago by
Aaron Lun
★ 28k • written 7.8 years ago by
sandmann.t
▴ 70
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Answer: Improving performance of edgeR and limma while adjusting for multiple confounder
by
Gordon Smyth
52k
We recommend that you use voomLmFit() instead of voom+lmFit. We are pretty proud of the speed of glmQLFit and voom, and we hadn't expected…
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68k
It appears that [SeSAMe][1] has functions meant to do that sort of thing. [1]: https://bioconductor.org/packages/release/bioc/vignet…
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by
Benjamin
▴ 20
I have received a response from Ensembl regarding the items I have listed above. I wanted to post it here and will continue to post update…
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by
Guido Hooiveld
★ 4.1k
Unfortunately you did not show how you generated your input data (the object `enrichment`), nor did you show the output from `sessionInfo()…
Answer: getBM from biomaRt inconsistencies found with .gtf files across multiple species
by
Mike Smith
★ 6.6k
I think this is because Ensembl can assign different Gene IDs to "the same gene" if it is found both on a standard chromosome and a scaffol…
Votes
Answer: Merging annotation with differential expression output
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clusterProfiler-GO enrichment Error
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