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simplesingleCell
•
reset
4
votes
5
replies
1.2k
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emptyDrops identified less non-empty cells with lower total UMI counts?
DropletUtils
scRNA-seq
emptyDrops
simplesinglecell
cell calling
updated 2.3 years ago by
Aaron Lun
★ 28k • written 3.4 years ago by
C T
▴ 140
4
votes
6
replies
723
views
Feature selection across batches in simpleSingleCell when spike-ins are not available.
simpleSingleCell
updated 3.4 years ago by
Aaron Lun
★ 28k • written 3.4 years ago by
Angelos Armen
• 0
1
vote
1
reply
377
views
Feature selection across batches in simpleSingleCell "Correcting batch effects" vignette
simpleSingleCell
updated 3.4 years ago by
Aaron Lun
★ 28k • written 3.4 years ago by
Angelos Armen
• 0
5
votes
1
reply
3.3k
views
Error with DropletUtils::EmptyDrops()
DropletUtils
EmptyDrops
simpleSingleCell
updated 5 months ago by
stefano.mangiola
▴ 10 • written 3.5 years ago by
shbrief
▴ 20
1
vote
5
replies
1.0k
views
Preprocessing in scran::doubletCells
scran
simpleSingleCell
updated 3.5 years ago by
Aaron Lun
★ 28k • written 3.5 years ago by
Angelos Armen
• 0
4
votes
4
replies
1.9k
views
using limma and edgeR for differential expression of Drop-seq/10xGenomics scRNA-seq data
limma
edgeR
simplesingleCell
scran
scater
updated 3.5 years ago by
Aaron Lun
★ 28k • written 3.5 years ago by
Bogdan
▴ 660
3
votes
2
replies
912
views
single cell data: adding random effects for subject with multilevel experiment design
SingleCellExperiment
scran
limma
simpleSingleCell
updated 3.6 years ago by
Aaron Lun
★ 28k • written 3.6 years ago by
Matt Mulé
• 0
1
vote
3
replies
1.1k
views
Troubles with barcodeRanks()
DropletUtils
SingleCellExperiement
DelayedArray
simpleSingleCell
updated 3.7 years ago by
Aaron Lun
★ 28k • written 3.7 years ago by
Steve Pederson
▴ 170
5
votes
2
replies
1.6k
views
Recommendations for combining multiple 10x runs into one SingleCellExperiment
singlecellexperiment
dropletutils
simplesinglecell
updated 4.3 years ago by
Aaron Lun
★ 28k • written 4.3 years ago by
Peter Hickey
▴ 630
0
votes
1
reply
565
views
Unusual graph for Identifying HVGs from the normalized log-expression
workflows
simplesinglecell
updated 4.4 years ago by
Aaron Lun
★ 28k • written 4.4 years ago by
es.azadian
• 0
0
votes
1
reply
696
views
The explanation of plotExplanatoryVariables plot
simplesinglecell
updated 4.9 years ago by
Aaron Lun
★ 28k • written 4.9 years ago by
Xingyu
• 0
1
vote
2
replies
1.3k
views
SingleCellExperiment object cannot be used by Scater package?
scater
singlecellexperiment
simplesinglecell
updated 4.9 years ago by
Aaron Lun
★ 28k • written 4.9 years ago by
Xingyu
• 0
3
votes
2
replies
2.5k
views
Sparse matrices in Bioconductor objects for single-cell analyses
scater
simpleSingleCell
workflows
updated 5.6 years ago by
Aaron Lun
★ 28k • written 5.6 years ago by
sandmann.t
▴ 70
13 results • Page
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Replies
Comment: Empty ChipQC Report
by
Okan
• 0
Hi, I have the same issue. Have you found any solution or any reason why this is happening?
Comment: edgeR: Usage of estimated dispersion from replicates for pools of the same exper
by
Gordon Smyth
47k
Am I understanding correctly that you have 24 RNA-seq libraries in total, 6 from the pooled samples and 18 from regular samples with biolog…
Comment: edgeR: Usage of estimated dispersion from replicates for pools of the same exper
by
k.roelfs
• 0
I'm sorry, I know it is kinda confusing... We have 6 pools in total: Phenotype A: T1a, T2, T1b, T1c Phenotype B: T1, T2 Each pool consist…
Comment: edgeR: Usage of estimated dispersion from replicates for pools of the same exper
by
Gordon Smyth
47k
Sorry, I don't understand your experiment. You are reading the edgeR Section on on what to do without replicates, but your experiment appe…
Answer: MA plot
by
Nathalie
• 0
Thank you very much for your help and comments. There is errors in my file. BaseMean is BaseMeanLog2. I don't know how insert a file.
Votes
Volcano Plot in R
Answer: Replicating iDEP parameters in DESeq2; two conditions and an interaction term.
Answer: Replicating iDEP parameters in DESeq2; two conditions and an interaction term.
Answer: MA plot
Answer: MA plot
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