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RNA seq
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reset
0
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9
replies
4.0k
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How to create manhattan plot with DESeq2
deseq2
mahattan plot
RNA Seq
updated 4.9 years ago by
bernatgel
▴ 150 • written 5.9 years ago by
adeler001
• 0
2
votes
5
replies
4.8k
views
Possible batch effect correction with RNA-Seq data when the batches do not contain both biological conditions for DE analysis
batch effect
limma
rna seq
DE
updated 5.4 years ago by
Steve Lianoglou
★ 13k • written 5.4 years ago by
svlachavas
▴ 840
0
votes
3
replies
1.4k
views
Normalization of RNA seq data
RNA seq
cpm
updated 5.4 years ago by
Kevin Blighe
★ 4.0k • written 5.4 years ago by
isabel.pieterse
• 0
4
votes
4
replies
1.4k
views
Custom gene annotations for SGSeq
sgseq
alternative splicing
rna seq
updated 13 months ago by
Sara
• 0 • written 5.8 years ago by
116100016
• 0
1
vote
4
replies
1.3k
views
Difference between Fold Changes from EdgeR glmLRTest and directly calculated fold changes.
edger
RNA Seq
updated 5.8 years ago by
James W. MacDonald
68k • written 5.8 years ago by
abf
▴ 30
0
votes
0
replies
788
views
searching for specific RNA sequences
rna seq
5.8 years ago
adeler001
• 0
0
votes
2
replies
1.3k
views
using only uniquely-mapping reads for alternative splicing using SGSeq
rna seq
SGSeq
Alternative splicing
updated 5.9 years ago by
Leonard Goldstein
▴ 260 • written 5.9 years ago by
116100016
• 0
0
votes
1
reply
786
views
51 views DESeq2 RNAseq differential expression analysis
deseq2
likelihood ratio
rna seq
wald test
updated 6.0 years ago by
Michael Love
43k • written 6.0 years ago by
adeler001
• 0
0
votes
2
replies
1.3k
views
Need help cprrecting overlappinge genes on valcano plot
volcano plot
rna seq
updated 6.1 years ago by
Michael Love
43k • written 6.1 years ago by
adeler001
• 0
1
vote
5
replies
1.8k
views
Time course experiment using limma with voom
limma
voom
RNA Seq
Time-course experiments
updated 6.2 years ago by
Aaron Lun
★ 29k • written 6.2 years ago by
mahes.muniandy
• 0
5
votes
3
replies
1.6k
views
Steep dendrograms and few modules. WGCNA on RNA seq data
RNA seq
wgcna
updated 6.3 years ago by
Peter Langfelder
★ 3.0k • written 6.3 years ago by
agustin.gonvi
▴ 20
1
vote
2
replies
2.6k
views
adapter trimming paired vs unpaired reads
rna seq
trimmomatic
trimming
adapter trimming
paired analysis
6.4 years ago
Mozart
▴ 30
0
votes
0
replies
2.1k
views
Sub clustering from hierarchical cluster used to make heat map of gene expression data
heatmap.2
hierarchical clustering
microarray
RNA seq
DEG
7.6 years ago
lawarde.ankita1
▴ 30
13 results • Page
1 of 1
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Comment: Guidance on limma for pseudobulk scRNA-seq with multi-factor design
by
Gordon Smyth
53k
Normalization or filtering are never done for subsets of samples in any limma or edgeR analysis. These things must always be done globally.
Answer: DESeq2 interactions
by
swbarnes2
★ 1.4k
The simpler way to do the first four comparisons is to make a new column in colData that is temp_pollution, and specify that column alone a…
Comment: How to construct a BSgenome object from a custom genome assembly using BSgenomeF
by
James W. MacDonald
68k
You are. The reason I pointed you to `forgeBSgenomeDataPkgFromTwobitFile` is because it's much simpler, doesn't require a seed file, and is…
Comment: How to construct a BSgenome object from a custom genome assembly using BSgenomeF
by
mat149
▴ 80
I've tried the following, but I must be missing something: setwd("C:\\Users\\....\\wd\\dump\\seqs_srcdir") e122<-readDNA…
Comment: readBam vs read_bam_insert_metrics
by
rtrivedi1
▴ 10
Thanks for the answer.
Votes
Comment: How to construct a BSgenome object from a custom genome assembly using BSgenomeF
Answer: Guidance on limma for pseudobulk scRNA-seq with multi-factor design
Answer: How to left_join a tidySummarizedExperiment with a GRanges object by seqnames an
Answer: Limpa Pipeline Order
A: How barcode-plot enrichment is calculated?
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