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RNA seq
•
reset
0
votes
9
replies
3.3k
views
How to create manhattan plot with DESeq2
deseq2
mahattan plot
RNA Seq
updated 4.2 years ago by
bernatgel
▴ 150 • written 5.2 years ago by
adeler001
• 0
2
votes
5
replies
3.9k
views
Possible batch effect correction with RNA-Seq data when the batches do not contain both biological conditions for DE analysis
batch effect
limma
rna seq
DE
updated 4.7 years ago by
Steve Lianoglou
★ 13k • written 4.7 years ago by
svlachavas
▴ 840
0
votes
3
replies
939
views
Normalization of RNA seq data
RNA seq
cpm
updated 4.7 years ago by
Kevin Blighe
★ 4.0k • written 4.7 years ago by
isabel.pieterse
• 0
4
votes
4
replies
1.2k
views
Custom gene annotations for SGSeq
sgseq
alternative splicing
rna seq
updated 4 months ago by
Sara
• 0 • written 5.1 years ago by
116100016
• 0
1
vote
4
replies
1.1k
views
Difference between Fold Changes from EdgeR glmLRTest and directly calculated fold changes.
edger
RNA Seq
updated 5.1 years ago by
James W. MacDonald
67k • written 5.1 years ago by
abf
▴ 30
0
votes
0
replies
653
views
searching for specific RNA sequences
rna seq
5.1 years ago
adeler001
• 0
0
votes
2
replies
995
views
using only uniquely-mapping reads for alternative splicing using SGSeq
rna seq
SGSeq
Alternative splicing
updated 5.2 years ago by
Leonard Goldstein
▴ 260 • written 5.2 years ago by
116100016
• 0
0
votes
1
reply
673
views
51 views DESeq2 RNAseq differential expression analysis
deseq2
likelihood ratio
rna seq
wald test
updated 5.3 years ago by
Michael Love
43k • written 5.3 years ago by
adeler001
• 0
0
votes
2
replies
1.2k
views
Need help cprrecting overlappinge genes on valcano plot
volcano plot
rna seq
updated 5.3 years ago by
Michael Love
43k • written 5.3 years ago by
adeler001
• 0
1
vote
5
replies
1.5k
views
Time course experiment using limma with voom
limma
voom
RNA Seq
Time-course experiments
updated 5.5 years ago by
Aaron Lun
★ 28k • written 5.5 years ago by
mahes.muniandy
• 0
5
votes
3
replies
1.3k
views
Steep dendrograms and few modules. WGCNA on RNA seq data
RNA seq
wgcna
updated 5.6 years ago by
Peter Langfelder
★ 3.0k • written 5.6 years ago by
agustin.gonvi
▴ 20
1
vote
2
replies
2.3k
views
adapter trimming paired vs unpaired reads
rna seq
trimmomatic
trimming
adapter trimming
paired analysis
5.6 years ago
Mozart
▴ 30
0
votes
0
replies
1.8k
views
Sub clustering from hierarchical cluster used to make heat map of gene expression data
heatmap.2
hierarchical clustering
microarray
RNA seq
DEG
6.8 years ago
lawarde.ankita1
▴ 30
13 results • Page
1 of 1
Recent ...
Replies
Answer: Cross-validation with multiple control subgroups in limma
by
Gordon Smyth
52k
limma doesn't do cross-validation or resampling. Like most classical linear modeling procedures in statistics, limma uses a statistical mod…
Comment: Issues with seqlevelsStyle when making custom txdb objects for genomes/annotatio
by
Robert Castelo
★ 3.4k
Hi Hervé, thank you very much for your input into how to properly build and manage TxDb objects. gDNAx has two main inputs, one or more BAM…
Comment: Biostrings: Error writing long reads (>200 kbps) with writeQualityScaledXStringS
by
Aidan
▴ 60
Quick update: this is fixed in a branch awaiting PR to Biostrings (https://github.com/Bioconductor/Biostrings/pull/122). Should be in `deve…
Answer: Can DESeq2's design compensate for sequencing experimental design shortcomings?
by
Michael Love
43k
For statistical analysis plans, I recommend working with a local statistician or someone familiar with linear models in R. I have to reserv…
Comment: how should I apply "cpg.annotate" to TCGA methylation data in hg38 for HM450K?
by
xiaofeiwang198266
• 0
Thanks for your reply!
Votes
C: edgeR and lack of counts ID on CPM matrix
A: DESeq2 Following RSEM
A: importing RSEM data into DESeq2
A: Expected counts from RSEM in DESeq2
A: Using RSEM reads for DESeq2
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