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RNA seq
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9
replies
3.4k
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How to create manhattan plot with DESeq2
deseq2
mahattan plot
RNA Seq
updated 4.3 years ago by
bernatgel
▴ 150 • written 5.3 years ago by
adeler001
• 0
2
votes
5
replies
4.0k
views
Possible batch effect correction with RNA-Seq data when the batches do not contain both biological conditions for DE analysis
batch effect
limma
rna seq
DE
updated 4.8 years ago by
Steve Lianoglou
★ 13k • written 4.8 years ago by
svlachavas
▴ 840
0
votes
3
replies
1.0k
views
Normalization of RNA seq data
RNA seq
cpm
updated 4.8 years ago by
Kevin Blighe
★ 4.0k • written 4.8 years ago by
isabel.pieterse
• 0
4
votes
4
replies
1.2k
views
Custom gene annotations for SGSeq
sgseq
alternative splicing
rna seq
updated 6 months ago by
Sara
• 0 • written 5.2 years ago by
116100016
• 0
1
vote
4
replies
1.1k
views
Difference between Fold Changes from EdgeR glmLRTest and directly calculated fold changes.
edger
RNA Seq
updated 5.2 years ago by
James W. MacDonald
67k • written 5.2 years ago by
abf
▴ 30
0
votes
0
replies
669
views
searching for specific RNA sequences
rna seq
5.2 years ago
adeler001
• 0
0
votes
2
replies
1.0k
views
using only uniquely-mapping reads for alternative splicing using SGSeq
rna seq
SGSeq
Alternative splicing
updated 5.3 years ago by
Leonard Goldstein
▴ 260 • written 5.3 years ago by
116100016
• 0
0
votes
1
reply
688
views
51 views DESeq2 RNAseq differential expression analysis
deseq2
likelihood ratio
rna seq
wald test
updated 5.4 years ago by
Michael Love
43k • written 5.4 years ago by
adeler001
• 0
0
votes
2
replies
1.2k
views
Need help cprrecting overlappinge genes on valcano plot
volcano plot
rna seq
updated 5.4 years ago by
Michael Love
43k • written 5.4 years ago by
adeler001
• 0
1
vote
5
replies
1.6k
views
Time course experiment using limma with voom
limma
voom
RNA Seq
Time-course experiments
updated 5.6 years ago by
Aaron Lun
★ 28k • written 5.6 years ago by
mahes.muniandy
• 0
5
votes
3
replies
1.3k
views
Steep dendrograms and few modules. WGCNA on RNA seq data
RNA seq
wgcna
updated 5.7 years ago by
Peter Langfelder
★ 3.0k • written 5.7 years ago by
agustin.gonvi
▴ 20
1
vote
2
replies
2.4k
views
adapter trimming paired vs unpaired reads
rna seq
trimmomatic
trimming
adapter trimming
paired analysis
5.8 years ago
Mozart
▴ 30
0
votes
0
replies
1.9k
views
Sub clustering from hierarchical cluster used to make heat map of gene expression data
heatmap.2
hierarchical clustering
microarray
RNA seq
DEG
7.0 years ago
lawarde.ankita1
▴ 30
13 results • Page
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Comment: Is there a way to do DEG analysis across two batches sharing one sample only?
by
kahunroleh
• 0
Yes, you can perform DEG analysis across two batches with one shared sample by using batch correction methods like ComBat or limma. These t…
Comment: displaying the differential expression with deseq2 over 4X2 samples with the in
by
Martijn
• 0
thanks James, but if I do pairwise analyses per subject, I get the impression that I only get the intercepts per gene. Do you mean regardin…
Comment: plyranges: find exact GRanges match keeping metadata columns
by
alexis.weinreb
• 0
Thank you, this does clarify the available options.
Answer: displaying the differential expression with deseq2 over 4X2 samples with the in
by
James W. MacDonald
67k
If you have paired data you should block on subject, which is algebraically the same as fitting a model on the difference between treated a…
Answer: How to compare expression levels of genes between scRNAseq and bulkRNAseq?
by
jodan
• 0
Your approach sounds solid! You might also consider plotting a scatter plot comparing log2 fold changes from both datasets to identify gene…
Votes
Answer: Find exact GRanges match keeping metadata columns
Answer: Find exact GRanges match keeping metadata columns
Answer: Unused arguments error without used arguments in GSVA?
Answer: Unused arguments error without used arguments in GSVA?
Answer: Unused arguments error without used arguments in GSVA?
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