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mast
•
reset
5
votes
3
replies
2.6k
views
single-cells RNA-seq analysis by MAST with unbalanced group size
mast
single-cell
rna-seq
differential analysis
updated 25 days ago by
achoppe
▴ 10 • written 7.3 years ago by
shao
▴ 100
4
votes
1
reply
2.2k
views
NAs in continuous coefficient and fold change in `zlm`
scrnaseq
MAST
differential gene expression
7.7 years ago
Andrew_McDavid
▴ 280
3
votes
2
replies
2.8k
views
MAST reported warning "Coefficients ... are never estimible and will be dropped."
mast
sc-rnaseq
8.1 years ago • updated 8.0 years ago
shao
▴ 100
3
votes
3
replies
3.2k
views
How do you test a contrast in MAST?
MAST
single-cell
differential gene expression
updated 7.6 years ago by
Andrew_McDavid
▴ 280 • written 7.6 years ago by
Shian Su
▴ 40
3
votes
3
replies
1.2k
views
Robust way of dealing with low number of samples for Differential Gene Expression
limma
edgeR
DESeq2
MAST
written 6 months ago by
Satoshi
• 0
2
votes
4
replies
2.4k
views
Interpretation of cngeneson when doing differential expression analysis in MAST
mast
differential gene expression
updated 7.0 years ago by
Andrew_McDavid
▴ 280 • written 7.0 years ago by
francesco.brundu@gmail.com
▴ 40
2
votes
3
replies
1.6k
views
Logistic regression in MAST - question
mast
updated 6.1 years ago by
Andrew_McDavid
▴ 280 • written 6.1 years ago by
Nik Tuzov
▴ 90
2
votes
3
replies
2.6k
views
Using MAST with DropSeq data
MAST
mast
dropseq
updated 7.2 years ago by
Andrew_McDavid
▴ 280 • written 7.2 years ago by
jeremycfd
▴ 10
2
votes
1
reply
5.0k
views
Intepretation of Z scores for gene set enrichment analysis in MAST
MAST
scRNA
GSEA
updated 7.7 years ago by
Andrew_McDavid
▴ 280 • written 7.8 years ago by
siajunren
• 0
2
votes
1
reply
3.2k
views
MAST: testing differential expression with a nested factorial design
MAST
single-cell
rna-seq
updated 7.0 years ago by
Andrew_McDavid
▴ 280 • written 7.1 years ago by
khandarius
▴ 20
2
votes
1
reply
1.3k
views
How to load data csv in MAST
mast
7.0 years ago
francesco.brundu@gmail.com
▴ 40
2
votes
7
replies
2.2k
views
Estimation of non-trivial contrasts in MAST
mast
updated 6.1 years ago by
Andrew_McDavid
▴ 280 • written 6.1 years ago by
Nik Tuzov
▴ 90
2
votes
2
replies
2.6k
views
Input for MAST
MAST
2.2 years ago • updated 2.1 years ago
zuljiamel1991
▴ 10
1
vote
3
replies
1.8k
views
comparing 2 models with confounders with MAST (scRNA-seq DEA)
scRNA-seq
MAST
confounders
updated 5.3 years ago by
Andrew_McDavid
▴ 280 • written 5.3 years ago by
ramonmassoni
▴ 10
1
vote
2
replies
924
views
Extracting genomic sequences using agat and bedtools yields different results
MAST
updated 13 months ago by
ATpoint
★ 4.8k • written 13 months ago by
maximilian.beikirch
• 0
1
vote
7
replies
2.2k
views
Likelihood ratio test (lrTest) in MAST comparing full to reduced model; and another question on controlling for CDR
MAST
2.1 years ago
Ken C
• 0
1
vote
0
replies
875
views
When I run `zlm` with several clusters, how to compare 1 cluster versus all the others?
MAST
3.7 years ago
Dan
• 0
0
votes
0
replies
1.1k
views
Issues geting meaninfull results for single cell DGE using MAST
MAST
SingleCell
3.3 years ago
zroger499
• 0
0
votes
1
reply
1.4k
views
Low logFCs using MAST for scRNA-seq
MAST
updated 8 months ago by
nhaus
▴ 70 • written 3.1 years ago by
learner_MD
• 0
0
votes
0
replies
814
views
How to extract P-Values and LogFC from lrTest in MAST?
pvalue
logFC
lrtest
MAST
2.0 years ago
Tadeoye
▴ 20
0
votes
1
reply
632
views
Which input data is acceptable for MAST?
SCTransform
MAST
updated 6 months ago by
Andrew_McDavid
▴ 280 • written 6 months ago by
james.watson1
• 0
0
votes
0
replies
842
views
Error: grouping factors must have > 1 sampled level
Mast
mastR
MAST
updated 13 months ago by
James W. MacDonald
68k • written 13 months ago by
Andrew
• 0
0
votes
0
replies
381
views
MAST package: Most appropriate way to perform lrTest for effect of factor with multiple levels in pairwise manner?
MAST
9 months ago
Kelly
• 0
0
votes
0
replies
683
views
MAST for single cell ATAC data
MAST
2.2 years ago
zuljiamel1991
▴ 10
0
votes
0
replies
415
views
Comparing the effects of a condition over time in two seperate tissues
MAST
14 months ago
Dillon
• 0
0
votes
0
replies
940
views
Differential expression with single cell data: any good two component (hurdle) models recently?
SingleCellWorkflow
DifferentialExpression
SingleCell
SingleCellMultiModal
MAST
2.8 years ago
Nik Tuzov
▴ 90
0
votes
0
replies
757
views
How does MAST caculate log fc
MAST
2.7 years ago
Chenghao
• 0
0
votes
1
reply
1.3k
views
Differentially expressed genes across three different conditions
MAST
SingleCell
diffGeneAnalysis
updated 3.8 years ago by
James W. MacDonald
68k • written 3.8 years ago by
sofia.puvogel
• 0
0
votes
0
replies
1.1k
views
Performing DGE analysis with MAST on a variable with more than 2 levels
MAST
DEG
SingleCell
3.0 years ago
zroger499
• 0
0
votes
0
replies
599
views
Linear Mixed Model for Differential Gene Expression in Single-Cell RNAseq with Batch Effects
lmer
MAST
DifferentialExpression
9 months ago
nhaus
▴ 70
0
votes
0
replies
180
views
Confidence interval contains 0 but p<0.05
MAST
4 months ago
Adelyn
• 0
0
votes
8
replies
2.4k
views
MAST - not getting Hurdle p values
MAST
updated 7.2 years ago by
Andrew_McDavid
▴ 280 • written 7.2 years ago by
jka8119
▴ 10
0
votes
0
replies
402
views
Z-score Interpretation across components
MAST
7 months ago
GJ
• 0
0
votes
3
replies
1.9k
views
MAST invalid Hypothesis, class objectORconnection error
R
MAST
scRNASeq
4.7 years ago
TriS
▴ 200
0
votes
1
reply
1.6k
views
Removal of batch effects before non-linear clustering for scRNAseq
scrnaseq
MAST
8.0 years ago
Andrew_McDavid
▴ 280
0
votes
3
replies
1.9k
views
Modeling Dose-response data in MAST
MAST
sc-rnaseq
scrnaseq
updated 7.8 years ago by
Andrew_McDavid
▴ 280 • written 7.8 years ago by
eleanorahowe
• 0
0
votes
2
replies
1.7k
views
What is the most useful distance measure to use for cell clustering based on MAST deviance residuals?
MAST
7.6 years ago
alexanderaivazidis
• 0
0
votes
4
replies
1.7k
views
MAST: What does the function gseaAfterBoot do exactly?
MAST
7.1 years ago
dfm2129
• 0
0
votes
6
replies
2.0k
views
Error in zlm function model matrix
MAST
7.0 years ago
jka8119
▴ 10
0
votes
2
replies
1.6k
views
Warning in bayes fit in MAST GSEA
MAST
GSEA
bayesglm.fit
6.6 years ago
santos22903
• 0
0
votes
1
reply
1.4k
views
How can I compute AIC for MAST hurdle model fitted in the whole dataset?
MAST
AIC calcuation
Parameters
single cell
hurdle model
updated 13 months ago by
rproendo
▴ 30 • written 6.2 years ago by
mcalgaro93
• 0
0
votes
1
reply
2.4k
views
Minimum number of cells required for the MAST
MAST
scRNAseq
differential expression
5.6 years ago
Andrew_McDavid
▴ 280
0
votes
0
replies
1.2k
views
DEG among experimental and control group in MAST
MAST
DEG
batch effects
5.6 years ago
chendianyu
• 0
0
votes
1
reply
1.9k
views
About getting DE genes using MAST
MAST
updated 5.3 years ago by
Andrew_McDavid
▴ 280 • written 5.3 years ago by
ahmed.abbas
• 0
0
votes
2
replies
1.6k
views
MAST compare two conditions with several donors
MAST
updated 4.2 years ago by
Emanuele
• 0 • written 4.5 years ago by
sofia.puvogel
• 0
0
votes
1
reply
1.6k
views
How to do pairwise multiple comparisons in MAST
MAST
updated 9 months ago by
Kelly
• 0 • written 4.2 years ago by
sysumed3615
• 0
0
votes
1
reply
1.2k
views
MAST zlm shows warning: Coefficients ... are never estimible and will be dropped.
MAST
updated 3.9 years ago by
shamsudheen
• 0 • written 4.2 years ago by
Helene
• 0
0
votes
1
reply
807
views
Mismatch between MAST DE results and expression dot plot
MAST
mastR
15 months ago
Simon
• 0
0
votes
0
replies
1.6k
views
How to interpret MAST coefficients
DifferentialExpression
MAST
2.5 years ago
Ismail
• 0
49 results • Page
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Comment: GSEA preranked analysis downstream of DEseq2
by
Ajay
• 0
`logFC * -log10(pval)` seems to be the pi-score proposed in this [paper][1] [1]: https://pmc.ncbi.nlm.nih.gov/articles/PMC3957066/
Comment: error in ChAMP loading file
by
janwilseo
• 0
Any update on this post? I also had an issue with this. [erosion control](https://charlestonhydroseeding.com/)
Answer: CluserProfiler message "No gene can be mapped"
by
James W. MacDonald
68k
Two things. First, the order of your term2gene is backwards (should be term and then gene, like the name), and by default `enricher` adjust…
Comment: Controlling for batch and estrus cycle using DESeq2 in RNA-seq analysis
by
donnycrimson
• 0
One potential workaround might be to adjust for these factors using a combination of design matrices perhaps including batch as a covariate…
Comment: DESeq2 Design controlling for gender
by
donnycrimson
• 0
On a lighter note, while you are tackling such complex data, you might enjoy a quick break playing the [Slope Game](https://slopegame.lol)…
Votes
DiffBind; Error: No sites have activity greater than filter value
remove X and Y chromosome genes in RNA-seq data using DESeq2 pipeline
How to remove X & Y chromosome genes from RNAseq data
Is it advisable to remove X and Y chromosome genes in mouse bulk RNA-seq data at the level of the count matrix?
A: Unbalanced experiment with multiple samples from each patient.
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