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CyTOF
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flow cytometry differential expression through time
cytofWorkflow
cytof
diffcyt
4.0 years ago
achaillon
• 0
0
votes
0
replies
1.7k
views
Error from FlowSom to Cytofast
Cytofast
FlowSom
CyTOF
fcs files
4.3 years ago
domenico.somma
• 0
1
vote
5
replies
1.3k
views
CyTOF Differential Abundance Analysis using DESeq2
deseq2
mass cytometry
CyTOF
updated 4.3 years ago by
Michael Love
43k • written 4.3 years ago by
mikhael.manurung
▴ 280
0
votes
0
replies
782
views
Concatenating different runs from the same pool of barcoded CyTOF samples
batch effect
cytof
4.8 years ago
mikhael.manurung
▴ 280
1
vote
6
replies
2.0k
views
Re: Cydar. Can I Normalize CyTOF data with just one FCS file/batch and if so, which normalization mode is recommended?
Cydar
CyTOF
normalization
updated 5.3 years ago by
Aaron Lun
★ 28k • written 5.3 years ago by
ghogg
• 0
2
votes
4
replies
1.1k
views
Cydar's countCells tolerance parameter for arcsinh-transformed data
cydar
CyTOF
updated 5.9 years ago by
Aaron Lun
★ 28k • written 5.9 years ago by
mikhael.manurung
▴ 280
2
votes
2
replies
1.5k
views
Cytofkit - Trouble with writing files after performing the analysis
cytofkit
cytof
write.csv
output
updated 8.2 years ago by
chen_hao
▴ 30 • written 8.2 years ago by
cjackson13
• 0
1
vote
2
replies
3.0k
views
Errors when reading CyToF flowSet into flowCore
flowCore
flowcore
CyToF
Rtsne
updated 9.4 years ago by
Jiang, Mike
★ 1.3k • written 9.4 years ago by
clevy
▴ 10
8 results • Page
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Comment: Why did PCA plots look exactly the same after adjusting for batch covariate in D
by
James W. MacDonald
67k
How you analyze your data is up to you. But do note that Subset * Conditions implies that you want to find genes that respond to stimulus d…
Comment: MBDseq analysis using qsea package
by
kf
• 0
Hi Matthias, Happy new year! I wondered if it is possible to retrieve normalized value from each samples? makeTable seems only return beta…
Comment: Why did PCA plots look exactly the same after adjusting for batch covariate in D
by
Quang
• 0
Hi, James. I forgot to mention, but yes, I did run `DESeq2::DESeq()`. So, I ran `DESeq2::DESeqDataSetFromHTSeqCount`, then `DESeq2::DESeq()…
Answer: Why did PCA plots look exactly the same after adjusting for batch covariate in D
by
ATpoint
★ 4.6k
It's an FAQ in the vignette: https://bioconductor.org/packages/release/bioc/vignettes/DESeq2/inst/doc/DESeq2.html#why-after-vst-are-there-s…
Comment: Deseq2, enrichGO and ensembl ID
by
James W. MacDonald
67k
I am not sure I understand. You say that a unique Ensembl ID maps to multiple gene symbols. That doesn't mean you have two genes (it's just…
Votes
A: Trajectory analysis for single cell sequencing
Answer: Annotation with org.Hs.eg.db Genome Build 38
Answer: Annotation with org.Hs.eg.db Genome Build 38
Answer: Considerations behind the choice of RNAseq Differential Expression Analysis Tool
A: goana limma- extract list of DE genes and genes in the enriched GO terms?
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