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simpleSingleCell
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reset
4
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5
replies
2.8k
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emptyDrops identified less non-empty cells with lower total UMI counts?
DropletUtils
scRNA-seq
emptyDrops
simplesinglecell
cell calling
updated 4.9 years ago by
Aaron Lun
★ 29k • written 6.0 years ago by
C T
▴ 140
4
votes
6
replies
1.8k
views
Feature selection across batches in simpleSingleCell when spike-ins are not available.
simpleSingleCell
updated 6.1 years ago by
Aaron Lun
★ 29k • written 6.1 years ago by
Angelos Armen
• 0
1
vote
1
reply
967
views
Feature selection across batches in simpleSingleCell "Correcting batch effects" vignette
simpleSingleCell
updated 6.1 years ago by
Aaron Lun
★ 29k • written 6.1 years ago by
Angelos Armen
• 0
5
votes
1
reply
5.4k
views
Error with DropletUtils::EmptyDrops()
DropletUtils
EmptyDrops
simpleSingleCell
updated 3.1 years ago by
Stefano Mangiola
▴ 40 • written 6.1 years ago by
shbrief
▴ 20
1
vote
5
replies
2.3k
views
Preprocessing in scran::doubletCells
scran
simpleSingleCell
updated 6.1 years ago by
Aaron Lun
★ 29k • written 6.1 years ago by
Angelos Armen
• 0
4
votes
4
replies
3.5k
views
using limma and edgeR for differential expression of Drop-seq/10xGenomics scRNA-seq data
limma
edgeR
simplesingleCell
scran
scater
updated 6.1 years ago by
Aaron Lun
★ 29k • written 6.1 years ago by
Bogdan
▴ 670
4
votes
2
replies
2.2k
views
single cell data: adding random effects for subject with multilevel experiment design
SingleCellExperiment
scran
limma
simpleSingleCell
updated 6.3 years ago by
Aaron Lun
★ 29k • written 6.3 years ago by
Matt Mulé
• 0
1
vote
3
replies
2.1k
views
Troubles with barcodeRanks()
DropletUtils
SingleCellExperiement
DelayedArray
simpleSingleCell
updated 6.3 years ago by
Aaron Lun
★ 29k • written 6.3 years ago by
Steve Pederson
▴ 170
8
votes
2
replies
3.5k
views
Recommendations for combining multiple 10x runs into one SingleCellExperiment
singlecellexperiment
dropletutils
simplesinglecell
updated 6.9 years ago by
Aaron Lun
★ 29k • written 6.9 years ago by
Peter Hickey
▴ 760
0
votes
1
reply
1.2k
views
Unusual graph for Identifying HVGs from the normalized log-expression
workflows
simplesinglecell
updated 7.0 years ago by
Aaron Lun
★ 29k • written 7.0 years ago by
es.azadian
• 0
0
votes
1
reply
1.3k
views
The explanation of plotExplanatoryVariables plot
simplesinglecell
updated 7.5 years ago by
Aaron Lun
★ 29k • written 7.5 years ago by
Xingyu
• 0
1
vote
2
replies
2.2k
views
SingleCellExperiment object cannot be used by Scater package?
scater
singlecellexperiment
simplesinglecell
updated 7.6 years ago by
Aaron Lun
★ 29k • written 7.6 years ago by
Xingyu
• 0
3
votes
2
replies
4.1k
views
Sparse matrices in Bioconductor objects for single-cell analyses
scater
simpleSingleCell
workflows
updated 8.2 years ago by
Aaron Lun
★ 29k • written 8.2 years ago by
sandmann.t
▴ 70
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Answer: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
Michael Love
43k
I'm not familiar with this usage, or code here ``` normFactors <- exp(-1 * offst(set2)) ``` In the RNA-seq gene-level workflow, we …
Answer: Which of apeglm and ashr may be more appropriate for pseudobulked DESeq2 analysi
by
Michael Love
43k
> apeglm appears much more aggressive in shrinkage, pushing many genes toward logFC = 0 > ashr maintains a gradient of shrinkage val…
Comment: voomLmFit fitted log-CPM values
by
Gordon Smyth
53k
The fitted values are on the log2CPM scale. I would have told you if they were not! I think you may be getting confused by the differences…
Comment: voomLmFit fitted log-CPM values
by
Lina
▴ 10
Is the output from fitted(v) in the log2 CPM scale? or CPM scale?
Answer: voomLmFit fitted log-CPM values
by
Gordon Smyth
53k
Fitted values are obtained from limma MArrayLM objects by ``` fitted(v) ```
Votes
A: How barcode-plot enrichment is calculated?
A: How barcode-plot enrichment is calculated?
Answer: ROAST compare results with complex design
How to account for solvent controls in DESeq2 when comparing follicular vs luteal phase?
Answer: How to account for solvent controls in DESeq2 when comparing follicular vs lutea
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