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Comment: Is there a way to do DEG analysis across two batches sharing one sample only?
by
georgeyu703
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Michael, Thank you very much!
Comment: Is there a way to do DEG analysis across two batches sharing one sample only?
by
Michael Love
43k
Yes, with the knowledge that, it is expected to be underpowered.
Comment: Is there a way to do DEG analysis across two batches sharing one sample only?
by
georgeyu703
•
0
Thank you, Michael. How? Could you provide more details? Can I just do it as usual, I mean to include batch in the model?
Answer: Is there a way to do DEG analysis across two batches sharing one sample only?
by
Michael Love
43k
The short answer is yes, you can use even a single sample to estimate the batch effect (and therefore control for it in otherwise confounde…
Comment: How to sort negative log2FC or FC in RNAseq for GSEA?
by
alserg
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280
`NES > 0` means that your gene set has more genes with positive logFC/stat. In your case these genes should be up in Subset1. However, if y…
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