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Showing :
TXIMPORT
•
reset
4
votes
5
replies
564
views
DESeq Input from Salmon + tximport/tximeta
tximportData
summarizeToGene
tximport
DESeq2
salmon
17 days ago
LuciaNhu
• 0
0
votes
2
replies
343
views
How can I obtain DEGs that are resulted from using salmon in alignment-based mode or mapping-based mode?
tximport
DESeq2
updated 7 weeks ago by
Michael Love
42k • written 7 weeks ago by
oguzhankaraosmanoglu
• 0
0
votes
2
replies
391
views
tximport data import
tximport
updated 11 weeks ago by
Michael Love
42k • written 11 weeks ago by
rodongo
• 0
1
vote
2
replies
744
views
How to use specific size factors and average transcript lengths from tximport in DESeq2?
DESeq2
tximport
3 months ago
grothmn
• 0
1
vote
2
replies
2.2k
views
using tximport with STAR transcript-quantification output
tximport
deseq2
STAR
updated 5 months ago by
rodrigo.duarte88
▴ 40 • written 5.1 years ago by
mohamedrefaat.1.1992
• 0
0
votes
4
replies
571
views
Create count matrix from multible quant sf files from different directories
tximportData
tximport
salmon
5 months ago
JG13
• 0
0
votes
2
replies
510
views
edgeR analysis: glm functions and offset matrix
tximport
edgeR
Normalization
7 months ago
Marianna
▴ 20
0
votes
2
replies
540
views
Clarification on Library Size Normalization After Using tximport with countsFromAbundance="lengthScaledTPM"
tximport
updated 8 months ago by
Michael Love
42k • written 8 months ago by
SG
• 0
2
votes
1
reply
475
views
Creating data matrix in Deseq2 from tximport data invalid class SummarizedExperiment object
DESeq2
tximport
updated 8 months ago by
ATpoint
★ 4.5k • written 8 months ago by
hemantcnaik
• 0
2
votes
3
replies
2.3k
views
Using STAR's readspergene.tab.out outputs to make gene-level count matrix for DESeq2 using tximport
STAR
tximport
RNASeq
DESeq2
updated 11 months ago by
swbarnes2
★ 1.4k • written 11 months ago by
Bo
• 0
0
votes
1
reply
669
views
tximport error with rsem files
rsem
tximport
updated 11 months ago by
ATpoint
★ 4.5k • written 11 months ago by
apolitics
• 0
3
votes
2
replies
944
views
tximport: Error in is(txi, "list") : argument "txi" is missing, with no default
tximport
DESeq2
updated 12 months ago by
James W. MacDonald
67k • written 12 months ago by
mckinlee.salazar
• 0
0
votes
3
replies
765
views
Tximport Error - transcripts in quant files are not the same as tx2gene
salmon
tximport
deseq2
updated 13 months ago by
Michael Love
42k • written 13 months ago by
Kayleigh
• 0
10
votes
11
replies
2.7k
views
tximport error: vroom
tximport
updated 13 months ago by
Abhishek
• 0 • written 13 months ago by
RLiu
▴ 40
2
votes
3
replies
1.2k
views
Import Salmon data with tximport and pool technical replicates
tximport
edgeR
written 15 months ago by
Vitor
• 0
4
votes
13
replies
2.5k
views
Does countsFromAbundance="lengthScaledTPM" produce un-normalized counts?
tximport
DESeq2
updated 14 months ago by
Michael Love
42k • written 15 months ago by
Christian
• 0
0
votes
4
replies
1.4k
views
Handle zero effective gene length when tximport RSEM results
tximport
edgeR
updated 15 months ago by
Michael Love
42k • written 15 months ago by
hanc
• 0
1
vote
2
replies
2.2k
views
IsoformSwitchAnalyzeR: What are the SV values created in design matrix?
salmon
IsoformSwitchAnalyzeR
tximport
RNASeqData
updated 16 months ago by
k.vitting.seerup
▴ 120 • written 16 months ago by
elva.gypsophila
▴ 10
2
votes
9
replies
1.7k
views
Importing RSEM processed data already formatted as a summarized experiment into DESeq2
tximport
tximportData
DESeq2
16 months ago
Ndimensional
▴ 20
0
votes
2
replies
904
views
tximport error in reading salmon output files?
tximport
tximportData
updated 9 months ago by
Matteo
• 0 • written 17 months ago by
barrypraveen
• 0
0
votes
1
reply
884
views
Which file in tximport should I use for DESeq2 analysis?
tximport
DESeq2
updated 18 months ago by
James W. MacDonald
67k • written 18 months ago by
Yijing
• 0
2
votes
1
reply
976
views
scaledTPM for DESeq2 using tximport
DESeq2
tximport
updated 18 months ago by
Michael Love
42k • written 18 months ago by
prangannathofficial
• 0
3
votes
7
replies
1.5k
views
failed to use tximport for creating count matrix from kallisto
tximport
kallisto
19 months ago • updated 18 months ago
Yijing
• 0
0
votes
2
replies
1.2k
views
Error in H5Fopen
rhdf5
Rhdf5lib
kallisto
tximport
19 months ago
Yijing
• 0
1
vote
3
replies
1.2k
views
tximport error
tximport
r
updated 19 months ago by
Michael Love
42k • written 19 months ago by
Joana
• 0
0
votes
2
replies
1.3k
views
combining multiple salmon runs after tximport
tximport
RNASeq
salmon
updated 21 months ago by
ATpoint
★ 4.5k • written 21 months ago by
Ivana
• 0
1
vote
5
replies
1.6k
views
tximport: kallisto + GENCODE transcript sanitization
tximport
updated 21 months ago by
Michael Love
42k • written 21 months ago by
Michael Steinbaugh
▴ 90
6
votes
10
replies
2.6k
views
Which input file is used for DGEList in EgdeR?
salmon
edgeR
tximport
nf-core
gene_counts
updated 7 months ago by
i.atanasoai
• 0 • written 21 months ago by
mohammedtoufiq91
▴ 10
0
votes
1
reply
945
views
Is it possible to convert transcript-level to gene-level dataset to use it in DESeq2 ?
DESeq2
Transcriptomics
tximport
deseq
txi
updated 22 months ago by
Dario Strbenac
★ 1.5k • written 22 months ago by
Hicham
▴ 10
0
votes
2
replies
1.1k
views
how to match salmon output to txname
GenomicFeatures
tximport
salmon
written 22 months ago by
camilia.savitri
• 0
0
votes
1
reply
1.1k
views
Missing transcript
DESeq2
tximport
salmon
updated 22 months ago by
ATpoint
★ 4.5k • written 22 months ago by
camilia.savitri
• 0
2
votes
1
reply
971
views
Confused about tximport-DESeq2 setup
DESeq2
tximport
salmon
updated 22 months ago by
ATpoint
★ 4.5k • written 22 months ago by
Dunois
• 0
2
votes
5
replies
1.9k
views
Normalizing Salmon output count data matrix using DESeq2
DESeq2
tximport
counts
Salmon
updated 9 months ago by
Michael Love
42k • written 23 months ago by
kiqbal
• 0
0
votes
2
replies
1.0k
views
Differentially expression analysis of orthologous genes between two species
DESeq2
tximport
24 months ago
Konstantinos
• 0
2
votes
3
replies
1.6k
views
Error while reading rsem files in tximport
DESeq2
tximport
rsem
RNASeq
tximportData
24 months ago
mohammedtoufiq91
▴ 10
4
votes
3
replies
1.4k
views
tx2gene object trouble when working with kallisto and A.thaliana
tx2gene
tximport
Arabidopsis_thaliana
kallisto
updated 2.0 years ago by
James W. MacDonald
67k • written 2.0 years ago by
Mikhail Biryukov
▴ 10
0
votes
1
reply
1.2k
views
Trouble with importing transcript counts with Salmon
Salmon
tximport
RNAseq
EdgeR
updated 2.0 years ago by
ATpoint
★ 4.5k • written 2.0 years ago by
Jenni
• 0
1
vote
2
replies
1.0k
views
error loading kallisto output in tximport
file.exists
false
kallisto
tximport
2.0 years ago
cta
• 0
2
votes
3
replies
1.7k
views
How to convert transcript counts to gene counts in R?
DESeq2
tximport
written 2.1 years ago by
L
▴ 50
3
votes
3
replies
1.3k
views
What is the correct way to import transcript abundances from 3' Quantseq results that calculated by kallisto?
tximport
tximportData
Quantseq
kallisto
2.1 years ago
duhwa.lee
• 0
1
vote
2
replies
2.0k
views
transcripts missing from tx2gene
tximport
2.2 years ago
Erick
• 0
0
votes
1
reply
866
views
tximport: not all(file.exists(files)) are true
tximport
updated 2.2 years ago by
Michael Love
42k • written 2.2 years ago by
凯
• 0
2
votes
1
reply
2.5k
views
Understanding tximport working with salmon outputs
Transcriptomics
tximport
updated 2.2 years ago by
ATpoint
★ 4.5k • written 2.2 years ago by
Florent
▴ 10
3
votes
4
replies
1.8k
views
difference between txi$counts and txi$abundance when countsFromAbundance is true
RNAseq
tximport
updated 2.2 years ago by
ATpoint
★ 4.5k • written 2.2 years ago by
snowmattin
• 0
0
votes
3
replies
1.2k
views
tximport error
error
tximport
updated 2.2 years ago by
Michael Love
42k • written 2.2 years ago by
hana
• 0
5
votes
6
replies
1.9k
views
Selecting individual samples from tximport and DGE matrix design with missing biological replicate
DESeq2
limma
tximport
updated 2.2 years ago by
Gordon Smyth
51k • written 2.2 years ago by
Kent
• 0
0
votes
4
replies
1.4k
views
Alternative for wininet method in tximeta
DESeq2
tximport
tximportData
tximeta
2.4 years ago
uhlkatie
• 0
1
vote
3
replies
2.2k
views
miRNA differential expression analysis - treat as transcript level?
tximport
DESeq2
edgeR
swish
miRNA
updated 2.4 years ago by
Michael Love
42k • written 2.4 years ago by
BG
• 0
2
votes
8
replies
2.0k
views
RSEM - tximport; where is effective_length information used? Implications for scRNA?
RSEM
tximport
scRNAseq
DESeq2
2.4 years ago
Kelen
▴ 10
0
votes
1
reply
1.3k
views
Function Request for Rsubread or tximport: parsing command-line featureCounts output
tximport
Rsubread
updated 2.5 years ago by
ATpoint
★ 4.5k • written 2.5 years ago by
johnmcma
▴ 10
50 results • Page
1 of 1
Recent ...
Replies
Comment: Help with RSEM counts: do I transpose matrix?
by
NB
• 0
OK thank you.
Comment: Help with RSEM counts: do I transpose matrix?
by
James W. MacDonald
67k
You can ask here, but please read the `DESeq2` vignette first, which should answer most questions.
Comment: Help with RSEM counts: do I transpose matrix?
by
NB
• 0
Thank you. If I have questions about QC/normalization, do I ask them here or ask a new question?
Answer: Help with RSEM counts: do I transpose matrix?
by
James W. MacDonald
67k
Yes. Samples in columns and genes in rows.
Comment: Issues with BSgenome.Mmusculus.UCSC.mm39 and generating motif matrix in Signac (
by
James W. MacDonald
67k
It's not informative except for me to say I was right that it's `getSeq` that is providing the error. It appears that `AddMotifs` is passin…
Votes
Answer: Detecting genomic signatures of drug resistance breast cancer in transcriptom
Comment: Issues with BSgenome.Mmusculus.UCSC.mm39 and generating motif matrix in Signac (
Answer: Issues with BSgenome.Mmusculus.UCSC.mm39 and generating motif matrix in Signac (
Comment: does anyone how to create rarefaction curves from a phyloseq object?
Answer: ANOVA like approach of edgeR
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