Log In
Sign Up
about
faq
Ask a question
Latest
News
Jobs
Tutorials
Tags
Users
New Post
Latest
News
Jobs
Tutorials
Tags
Users
Log In
Sign Up
About
Limit
all time
today
this week
this month
this year
Unanswered
All posts
Sort
Update
Answers
Bookmarks
Creation
Replies
Rank
Views
Votes
Showing :
deseq2
•
reset
1
vote
1
reply
564
views
i'm not getting any differentially expressed genes.
DESeq2
updated 19 months ago by
ATpoint
★ 5.0k • written 19 months ago by
Marilyn
• 0
2
votes
1
reply
782
views
Creating data matrix in Deseq2 from tximport data invalid class SummarizedExperiment object
DESeq2
tximport
updated 19 months ago by
ATpoint
★ 5.0k • written 19 months ago by
hemantcnaik
• 0
1
vote
1
reply
504
views
Perform PCA for sample clustering using DESeq2
DESeq2
updated 19 months ago by
ATpoint
★ 5.0k • written 19 months ago by
synat.keam
• 0
1
vote
3
replies
991
views
Cross-species analysis - how to compute the mid parent value?
DESeq2
limma
edgeR
DifferentialExpression
written 21 months ago by
pl23
• 0
0
votes
2
replies
910
views
DESeq2: Comparing A Knockout to a Strain
DESeq2
19 months ago
Viktor Thiel
• 0
0
votes
1
reply
814
views
DESeq2 for pseudobulk analysis of scCITE-seq
DESeq2
scCITE-seq
updated 19 months ago by
ATpoint
★ 5.0k • written 19 months ago by
daiane.hemerichbrennan
• 0
0
votes
8
replies
1.4k
views
DESeq and SVA for batch effect
DESeq2
BatchEffect
sva
19 months ago
michelafrancesconi8
• 0
0
votes
1
reply
646
views
Unusually large fold-changes in Deseq2 results
DESeq2
updated 19 months ago by
ATpoint
★ 5.0k • written 19 months ago by
Sneha
• 0
1
vote
4
replies
877
views
DESeq2 Replicates
DESeq2
RNASeq
19 months ago
kcarey
• 0
2
votes
6
replies
977
views
Factor vs Character in design
DESeq2
19 months ago
James Aiden
• 0
1
vote
3
replies
987
views
Dispersion estimation step is very slow with latest DESeq2 and glmGamPoi
DESeq2
glmGamPoi
20 months ago • updated 19 months ago
I-Hsuan Lin
▴ 10
4
votes
7
replies
1.8k
views
weird volcano plot
DESeq2
updated 19 months ago by
Shuning
• 0 • written 23 months ago by
shouzheng
▴ 10
0
votes
1
reply
696
views
DEseq2 dispersion plot
DESeq2
updated 19 months ago by
Michael Love
43k • written 20 months ago by
Emma
• 0
1
vote
3
replies
1.0k
views
DESEQ2 Batch Effect
DESeq2
BatchEffect
RNASeq
updated 20 months ago by
swbarnes2
★ 1.4k • written 20 months ago by
sap275
▴ 10
0
votes
2
replies
1.0k
views
Is DESeq2 normalisation appropriate when counts were obtained for a small number of genes?
DESeq2
updated 20 months ago by
ATpoint
★ 5.0k • written 20 months ago by
Suvi
• 0
0
votes
3
replies
868
views
Would I treat surrogate variables as batches?
DESeq2
sva
updated 20 months ago by
James W. MacDonald
68k • written 20 months ago by
MEME
▴ 10
0
votes
1
reply
651
views
Changed DESeq2 comparison value but the heatmap is still the same
DESeq2
pheatmap
updated 20 months ago by
James W. MacDonald
68k • written 20 months ago by
zhangaimei
• 0
1
vote
4
replies
967
views
Further clarification of unbalanced group sizes DESeq2
DESeq2
20 months ago
hmillike
• 0
0
votes
1
reply
455
views
few significant genes from DESeq2 analysis
DESeq2
updated 20 months ago by
Michael Love
43k • written 20 months ago by
Ming
• 0
1
vote
4
replies
1.4k
views
DEseq2 coefficient
DESeq2
coefficient
20 months ago • updated 18 months ago
JKim
• 0
3
votes
5
replies
1.7k
views
DESEQ Inquiry
DESeq2
rnaseqGene
RNASeqData
limma
RNASeq
updated 20 months ago by
Michael Love
43k • written 20 months ago by
sap275
▴ 10
2
votes
1
reply
717
views
Limma Question
DESeq2
BatchEffect
limma
RNASeq
updated 20 months ago by
Michael Love
43k • written 20 months ago by
sap275
▴ 10
2
votes
2
replies
801
views
locfunc not found in DESeq2
locfunc
DESeq2
shorth
estimateSizeFactors
20 months ago
Hope
▴ 10
1
vote
2
replies
823
views
evaluate the variable importance on genes
DESeq2
deseq
20 months ago
Meme
• 0
0
votes
3
replies
799
views
library sizes differ extremely after dds
DESeq2
updated 20 months ago by
Michael Love
43k • written 20 months ago by
HAK
• 0
1
vote
4
replies
1.5k
views
Comparing gene expression across three tissue types to identify any difference.
DESeq2
20 months ago
James Aiden
• 0
0
votes
13
replies
1.8k
views
Unexpected behavior of lfcShrink() function in DESeq2 analysis
DESeq2
SmallRNA
updated 20 months ago by
Michael Love
43k • written 20 months ago by
r.r.bioinf
• 0
1
vote
5
replies
953
views
vst transformation and zero counts
DESeq2
updated 20 months ago by
ATpoint
★ 5.0k • written 20 months ago by
boczniak767
▴ 740
1
vote
12
replies
2.6k
views
How to calculate p value when comparing two sets of DEGs and not applying interaction
DEGcomparison
DESeq2
InteractionSet
pvalue
20 months ago
paria.alipour
• 0
0
votes
3
replies
1.3k
views
utf-8 codec can't decode byte 0xd5
importr
DESeq2
updated 20 months ago by
Michael Love
43k • written 20 months ago by
yuhang
• 0
0
votes
1
reply
466
views
Problems with filtering DESeq Results (log2fold change)
DESeq2
updated 20 months ago by
ATpoint
★ 5.0k • written 20 months ago by
Aaliya
• 0
0
votes
3
replies
1.1k
views
DESeq2: Adjust for treatment effect patient vs control
DESeq2
updated 20 months ago by
Michael Love
43k • written 20 months ago by
Ead72bbe
• 0
0
votes
1
reply
1.8k
views
Pseudobulk-DESeq2 analyze multiple cell types at once?
pseudobulk
DESeq2
updated 20 months ago by
Michael Love
43k • written 20 months ago by
puffsquash
• 0
0
votes
7
replies
1.3k
views
DESeq filtering specific to contrasts
DESeq2
updated 18 months ago by
Michael Love
43k • written 20 months ago by
Emma
• 0
0
votes
2
replies
826
views
help checking the design matrix and contrast in DESeq2 "not full rank" scenario
DESeq2
20 months ago
ccshao
▴ 70
0
votes
1
reply
643
views
Double batch effect (Individuals nested within blocks in DESeq2)
DESeq2
updated 20 months ago by
Michael Love
43k • written 20 months ago by
Rania Ouaz
• 0
1
vote
2
replies
918
views
DESeq2 issue: can't create DESeq2 object
DESeq2
RBioinf
20 months ago
Magdalena
• 0
0
votes
1
reply
441
views
Inconsistency in DESeq2 Log fold change
DESeq2
updated 20 months ago by
ATpoint
★ 5.0k • written 20 months ago by
Kuldeep
• 0
0
votes
3
replies
741
views
DESeq2 Results in Different Pvalue & Log2FC using the same code and data
DESeq2
dese
written 20 months ago by
Xu
• 0
0
votes
5
replies
1.1k
views
DESEQ2 Repeated Measures-like design error help
DESeq2
updated 20 months ago by
Michael Love
43k • written 20 months ago by
DS
▴ 10
0
votes
3
replies
1.0k
views
DESeq2 interaction terms RIPseq analysis
DESeq2
RIPSeq
updated 20 months ago by
Michael Love
43k • written 20 months ago by
sg197
• 0
2
votes
2
replies
1.7k
views
Graphing DEseq2 Normalized Counts using ggplot2
DESeq2
Normalization
RNASeq
20 months ago
jackafiore
• 0
4
votes
2
replies
2.6k
views
How to do DESeq2 on Kallisto Data
DESeq2
Kallisto
updated 20 months ago by
dsull
▴ 40 • written 20 months ago by
Aaliya
• 0
1
vote
2
replies
2.4k
views
How to combine a bulk and pseudobulk dataset?
DESeq2
limma
RNASeq
RNASeqData
written 2.5 years ago by
informatician
• 0
0
votes
2
replies
712
views
Pairwise analysis of DESeq result and limma result
DESeq2
limma
20 months ago
Lily Cheang
▴ 10
0
votes
4
replies
844
views
DESeq2 nomalize value
DESeq2
20 months ago
史哲
• 0
0
votes
4
replies
1.8k
views
Should I use the 'log2FoldChange' from lfcShrink() to subset significant DEGs?
lfcshrink
DESeq2
written 22 months ago by
Igor
• 0
2
votes
1
reply
706
views
Continuous variable to identify responder genes using DESeq2
DESeq2
rnaseqGene
DifferentialExpression
updated 21 months ago by
Michael Love
43k • written 21 months ago by
Abhishek Singh
▴ 20
0
votes
3
replies
1.0k
views
LOG2FC DESEQ2
DESeq2
updated 21 months ago by
Michael Love
43k • written 21 months ago by
ilaria.montali
• 0
0
votes
1
reply
421
views
unequal number of treatmets to control
DESeq2
updated 21 months ago by
Michael Love
43k • written 21 months ago by
shawaimuhyees
• 0
4,835 results • Page
7 of 97
Recent ...
Replies
Answer: Using DESeq2 for age prediction
by
ATpoint
★ 5.0k
The package has no such inbuilt function but you are free to use the vst (or any normalized) counts to train a regression model or classifi…
Comment: Best practice for handling large data (matrix with >2^31-1 non-zero elements) in
by
James W. MacDonald
68k
Aaron already answered your question. If you have an HDF5 file, it will be read in as a `TENxMatrix` object, which isn't actually read in -…
Comment: Best practice for handling large data (matrix with >2^31-1 non-zero elements) in
by
dan.gatti
• 0
Very strange. We have the same package versions. I tried adding the mtx.class, type, and delayed arguments, but it's still making the coun…
Comment: DESeq2 for candidate gene analysis
by
piffelpaff
▴ 20
thank you so much for answering Dr. Love!
Answer: DESeq2 for candidate gene analysis
by
Michael Love
43k
Your plan 1 is reasonable. Run DESeq2 on the whole dataset to obtain size factors and moderated dispersion estimates and then extract the u…
Votes
A: Multi-level experiments in Limma
Answer: Should voomWithQualityWeights still be used when the sample-specific weights var
Answer: DESeq2 for candidate gene analysis
DESeq2 for candidate gene analysis
Answer: DESeq2 for candidate gene analysis
Awards
• All
Popular Question
to
Gordon Smyth
53k
Popular Question
to
Matej
• 0
Popular Question
to
Hervé Pagès
16k
Popular Question
to
Josef Frank
• 0
Popular Question
to
dan.gatti
• 0
Locations
• All
United States,
just now
United States,
12 minutes ago
United States,
20 minutes ago
United States,
27 minutes ago
United States,
34 minutes ago
Traffic: 855 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6