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fpkm
•
reset
3
votes
3
replies
3.0k
views
Differential expression analysis with only FPKM matrix available from total newbie in R
FPKM
RNASeqData
DEAnalysis
limma
19 months ago
Ethan Nguyen
• 0
2
votes
4
replies
2.2k
views
How to get TPM / FPKM after batch correction with DESeq2?
DESeq2
BatchEffect
tpm
fpkm
2.0 years ago
vk
• 0
0
votes
5
replies
5.4k
views
How to remove batch effect from RNA-seq without count data?
RUVSeq
FPKM
removeBatchEffect
RNA-seq
sva
updated 3.3 years ago by
ATpoint
★ 4.8k • written 4.1 years ago by
Xiaojie Cheng
• 0
1
vote
4
replies
5.6k
views
Conversion from FPKM,RPKM to tpm
DESEQ2
fpkm
rpkm
tpm
3.6 years ago
Sandhiya
• 0
0
votes
1
reply
2.6k
views
Doing differential expression using FPKM , samples don't have replication
dge
fpkm
rna-seq
updated 3.9 years ago by
Kevin Blighe
★ 4.0k • written 3.9 years ago by
lkianmehr
• 0
1
vote
4
replies
1.4k
views
tximport abundanceCol parameter not functioning for rsem data
tximport
rsem
transcript abundance
TPM
FPKM
updated 4.5 years ago by
Michael Love
43k • written 4.6 years ago by
m.metsger
• 0
2
votes
5
replies
16k
views
Conversion from counts to FPKM
counts
fpkm
fpkm()
normalization
deseq2
updated 18 months ago by
Hamza
• 0 • written 6.4 years ago by
chiara.facciotto
• 0
9
votes
5
replies
6.0k
views
Finding DE genes from RNA-seq data
edgeR
pvalue
qvalue
fpkm
differential gene expression
updated 6.5 years ago by
Steve Lianoglou
★ 13k • written 6.5 years ago by
Harrisonesmith
• 0
2
votes
4
replies
2.5k
views
why the logFC is different between edgeR,DESeq2 results and fpkm logFC(log2(FPKM_tumor_mean/FPKM_normal_mean)?
deseq2
edger
fpkm
counts
fc
updated 6.6 years ago by
Gordon Smyth
52k • written 6.6 years ago by
ZihaoXing
• 0
1
vote
2
replies
1.5k
views
how to output and get the normalization gene expression data (similar to the FPKM gene expression data) from reads count data by DESeq2 and edgeR?
deseq2
edger
fpkm
counts
TCGA
updated 6.6 years ago by
Gordon Smyth
52k • written 6.6 years ago by
ZihaoXing
• 0
0
votes
1
reply
1.8k
views
TPM and FPKM for lncRNA
GenomicFeatures
TPM
FPKM
lncRNA
6.9 years ago
Ina Hoeschele
▴ 620
4
votes
4
replies
6.2k
views
Best DEG tool for datasets with FPKM counts?
FPKM
7.5 years ago
Nithisha
▴ 10
1
vote
8
replies
3.8k
views
Add avgTxLength to DESeqDataSet
deseq2
fpkm
updated 3.8 years ago by
Michael Love
43k • written 7.7 years ago by
rhart
• 0
1
vote
1
reply
1.5k
views
FPKM normalization questions!
r
fpkm
bioinformatics
updated 8.5 years ago by
Aaron Lun
★ 28k • written 8.5 years ago by
fromhj304
▴ 10
5
votes
5
replies
3.5k
views
Why the log fold change of some genes returned by edgeR is not compatible with their related FPKM value?
edgeR
logFC
FPKM
updated 8.5 years ago by
Gordon Smyth
52k • written 8.5 years ago by
Sara
▴ 20
5
votes
9
replies
6.7k
views
Voom: need at least two non-NA values to interpolate
limma voom
voom
rsem
fpkm
8.6 years ago
komal.rathi
▴ 120
3
votes
4
replies
2.4k
views
Large differences in sample clustering using cummeRbund (csDendro) and rlog-distance (DESeq2)
deseq2
cummerbund
rlog
fpkm
clustering
9.3 years ago
Jon Bråte
▴ 260
2
votes
8
replies
4.9k
views
How to annotate DESeq2 object with gene size to get FPKMs
deseq2
fpkm
updated 9.7 years ago by
Michael Love
43k • written 9.7 years ago by
ashley.doane
▴ 20
7
votes
4
replies
3.0k
views
Gene expression between two copies of same gene:use counts or RPKM or FPKM?
count
rnaseq
rpkm
fpkm
edgeR
updated 10.0 years ago by
Gordon Smyth
52k • written 10.0 years ago by
thibault.lorin
▴ 10
19 results • Page
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Recent ...
Replies
Comment: Controlling for batch and estrus cycle using DESeq2 in RNA-seq analysis
by
donnycrimson
• 0
One potential workaround might be to adjust for these factors using a combination of design matrices perhaps including batch as a covariate…
Comment: DESeq2 Design controlling for gender
by
donnycrimson
• 0
On a lighter note, while you are tackling such complex data, you might enjoy a quick break playing the [Slope Game](https://slopegame.lol)…
Comment: MetaDE.pvalue: Error in xj[i]: invalid subscript type 'list'
by
donnycrimson
• 0
Have you considered streamlining how you handle data input to mitigate these issues. It might help to define the parameters more strictly …
Comment: CluserProfiler message "No gene can be mapped"
by
Carolina
• 0
There is, the overlap that reads out is ( Genes in common: 368 of 368 ). Here is the link for [background genes][1], [term2gene][2], [term2…
Comment: Differing results with DESeq2
by
JKim
• 0
My two cents. I think it would be more straightforward if you use cellmeans model. Have a look at [A guide to creating design matrices for …
Votes
remove X and Y chromosome genes in RNA-seq data using DESeq2 pipeline
How to remove X & Y chromosome genes from RNAseq data
Is it advisable to remove X and Y chromosome genes in mouse bulk RNA-seq data at the level of the count matrix?
A: Unbalanced experiment with multiple samples from each patient.
A: Understanding contrasts limma
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