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rna seq
•
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0
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9
replies
3.7k
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How to create manhattan plot with DESeq2
deseq2
mahattan plot
RNA Seq
updated 4.5 years ago by
bernatgel
▴ 150 • written 5.5 years ago by
adeler001
• 0
2
votes
5
replies
4.4k
views
Possible batch effect correction with RNA-Seq data when the batches do not contain both biological conditions for DE analysis
batch effect
limma
rna seq
DE
updated 5.0 years ago by
Steve Lianoglou
★ 13k • written 5.0 years ago by
svlachavas
▴ 840
0
votes
3
replies
1.2k
views
Normalization of RNA seq data
RNA seq
cpm
updated 5.0 years ago by
Kevin Blighe
★ 4.0k • written 5.0 years ago by
isabel.pieterse
• 0
4
votes
4
replies
1.3k
views
Custom gene annotations for SGSeq
sgseq
alternative splicing
rna seq
updated 8 months ago by
Sara
• 0 • written 5.4 years ago by
116100016
• 0
1
vote
4
replies
1.2k
views
Difference between Fold Changes from EdgeR glmLRTest and directly calculated fold changes.
edger
RNA Seq
updated 5.4 years ago by
James W. MacDonald
68k • written 5.4 years ago by
abf
▴ 30
0
votes
0
replies
717
views
searching for specific RNA sequences
rna seq
5.4 years ago
adeler001
• 0
0
votes
2
replies
1.2k
views
using only uniquely-mapping reads for alternative splicing using SGSeq
rna seq
SGSeq
Alternative splicing
updated 5.5 years ago by
Leonard Goldstein
▴ 260 • written 5.5 years ago by
116100016
• 0
0
votes
1
reply
732
views
51 views DESeq2 RNAseq differential expression analysis
deseq2
likelihood ratio
rna seq
wald test
updated 5.6 years ago by
Michael Love
43k • written 5.6 years ago by
adeler001
• 0
0
votes
2
replies
1.2k
views
Need help cprrecting overlappinge genes on valcano plot
volcano plot
rna seq
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
adeler001
• 0
1
vote
5
replies
1.6k
views
Time course experiment using limma with voom
limma
voom
RNA Seq
Time-course experiments
updated 5.9 years ago by
Aaron Lun
★ 28k • written 5.9 years ago by
mahes.muniandy
• 0
5
votes
3
replies
1.5k
views
Steep dendrograms and few modules. WGCNA on RNA seq data
RNA seq
wgcna
updated 5.9 years ago by
Peter Langfelder
★ 3.0k • written 5.9 years ago by
agustin.gonvi
▴ 20
1
vote
2
replies
2.5k
views
adapter trimming paired vs unpaired reads
rna seq
trimmomatic
trimming
adapter trimming
paired analysis
6.0 years ago
Mozart
▴ 30
0
votes
0
replies
2.0k
views
Sub clustering from hierarchical cluster used to make heat map of gene expression data
heatmap.2
hierarchical clustering
microarray
RNA seq
DEG
7.2 years ago
lawarde.ankita1
▴ 30
13 results • Page
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Comment: GSEA preranked analysis downstream of DEseq2
by
Ajay
• 0
`logFC * -log10(pval)` seems to be the pi-score proposed in this [paper][1] [1]: https://pmc.ncbi.nlm.nih.gov/articles/PMC3957066/
Comment: error in ChAMP loading file
by
janwilseo
• 0
Any update on this post? I also had an issue with this. [erosion control](https://charlestonhydroseeding.com/)
Answer: CluserProfiler message "No gene can be mapped"
by
James W. MacDonald
68k
Two things. First, the order of your term2gene is backwards (should be term and then gene, like the name), and by default `enricher` adjust…
Comment: Controlling for batch and estrus cycle using DESeq2 in RNA-seq analysis
by
donnycrimson
• 0
One potential workaround might be to adjust for these factors using a combination of design matrices perhaps including batch as a covariate…
Comment: DESeq2 Design controlling for gender
by
donnycrimson
• 0
On a lighter note, while you are tackling such complex data, you might enjoy a quick break playing the [Slope Game](https://slopegame.lol)…
Votes
DiffBind; Error: No sites have activity greater than filter value
remove X and Y chromosome genes in RNA-seq data using DESeq2 pipeline
How to remove X & Y chromosome genes from RNAseq data
Is it advisable to remove X and Y chromosome genes in mouse bulk RNA-seq data at the level of the count matrix?
A: Unbalanced experiment with multiple samples from each patient.
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