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rna seq
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reset
0
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9
replies
4.3k
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How to create manhattan plot with DESeq2
deseq2
mahattan plot
RNA Seq
updated 5.3 years ago by
bernatgel
▴ 150 • written 6.3 years ago by
adeler001
• 0
2
votes
5
replies
5.3k
views
Possible batch effect correction with RNA-Seq data when the batches do not contain both biological conditions for DE analysis
batch effect
limma
rna seq
DE
updated 5.8 years ago by
Steve Lianoglou
★ 13k • written 5.8 years ago by
svlachavas
▴ 840
0
votes
3
replies
1.6k
views
Normalization of RNA seq data
RNA seq
cpm
updated 5.8 years ago by
Kevin Blighe
★ 4.0k • written 5.8 years ago by
isabel.pieterse
• 0
4
votes
4
replies
1.6k
views
Custom gene annotations for SGSeq
sgseq
alternative splicing
rna seq
updated 17 months ago by
Sara
• 0 • written 6.2 years ago by
116100016
• 0
1
vote
4
replies
1.4k
views
Difference between Fold Changes from EdgeR glmLRTest and directly calculated fold changes.
edger
RNA Seq
updated 6.2 years ago by
James W. MacDonald
68k • written 6.2 years ago by
abf
▴ 30
0
votes
0
replies
848
views
searching for specific RNA sequences
rna seq
6.2 years ago
adeler001
• 0
0
votes
2
replies
1.4k
views
using only uniquely-mapping reads for alternative splicing using SGSeq
rna seq
SGSeq
Alternative splicing
updated 6.3 years ago by
Leonard Goldstein
▴ 260 • written 6.3 years ago by
116100016
• 0
0
votes
1
reply
871
views
51 views DESeq2 RNAseq differential expression analysis
deseq2
likelihood ratio
rna seq
wald test
updated 6.4 years ago by
Michael Love
43k • written 6.4 years ago by
adeler001
• 0
0
votes
2
replies
1.6k
views
Need help cprrecting overlappinge genes on valcano plot
volcano plot
rna seq
updated 6.4 years ago by
Michael Love
43k • written 6.4 years ago by
adeler001
• 0
1
vote
5
replies
2.0k
views
Time course experiment using limma with voom
limma
voom
RNA Seq
Time-course experiments
updated 6.6 years ago by
Aaron Lun
★ 29k • written 6.6 years ago by
mahes.muniandy
• 0
5
votes
3
replies
1.8k
views
Steep dendrograms and few modules. WGCNA on RNA seq data
RNA seq
wgcna
updated 6.6 years ago by
Peter Langfelder
★ 3.0k • written 6.6 years ago by
agustin.gonvi
▴ 20
1
vote
2
replies
2.8k
views
adapter trimming paired vs unpaired reads
rna seq
trimmomatic
trimming
adapter trimming
paired analysis
6.7 years ago
Mozart
▴ 30
0
votes
0
replies
2.2k
views
Sub clustering from hierarchical cluster used to make heat map of gene expression data
heatmap.2
hierarchical clustering
microarray
RNA seq
DEG
7.9 years ago
lawarde.ankita1
▴ 30
13 results • Page
1 of 1
Recent ...
Replies
Comment: Combining two proteomics datasets with limpa
by
Andrew Pattison
• 0
Thanks heaps Gordon. I went with option 1 and no normalisation and all seems to have worked well. Cheers, Andrew
Comment: limpa-blank normalization and Spectronaut's PTM stoichiometry
by
Gordon Smyth
53k
Flagging protein groups as likely contaminants in this way will be fine for a limpa analysis. The `Cont_` proteins can optionally stay in f…
Answer: Making a Full Rank Model for Allele-Specific Expression with DESeq2
by
Gordon Smyth
53k
Aaron Lun was a PhD student in my Lab back in 2015, when he posted the answers about allele-specific expression that you and Mike Love have…
Comment: Making a Full Rank Model for Allele-Specific Expression with DESeq2
by
Michael Love
43k
You just need the mouse identifier (controls baseline) and the group specific allelic effect.
Comment: limpa-blank normalization and Spectronaut's PTM stoichiometry
by
Julia Broadbent
• 0
Hi Emily, Just sharing a resource for handling contaminants - we use the methods described in [Frankenfield et al. (2022)](https://pubs.acs…
Votes
A: Filtering read counts matrix: how to deal with duplicated gene symbols, differen
Comment: limpa analysis advice
Answer: When to use edgeR or limma
Answer: When to use edgeR or limma
Answer: When to use edgeR or limma
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