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simplesingleCell
•
reset
4
votes
5
replies
3.2k
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emptyDrops identified less non-empty cells with lower total UMI counts?
DropletUtils
scRNA-seq
emptyDrops
simplesinglecell
cell calling
updated 5.3 years ago by
Aaron Lun
★ 29k • written 6.5 years ago by
C T
▴ 140
4
votes
6
replies
2.0k
views
Feature selection across batches in simpleSingleCell when spike-ins are not available.
simpleSingleCell
updated 6.5 years ago by
Aaron Lun
★ 29k • written 6.5 years ago by
Angelos Armen
• 0
1
vote
1
reply
1.1k
views
Feature selection across batches in simpleSingleCell "Correcting batch effects" vignette
simpleSingleCell
updated 6.5 years ago by
Aaron Lun
★ 29k • written 6.5 years ago by
Angelos Armen
• 0
5
votes
1
reply
5.7k
views
Error with DropletUtils::EmptyDrops()
DropletUtils
EmptyDrops
simpleSingleCell
updated 3.5 years ago by
Stefano Mangiola
▴ 40 • written 6.5 years ago by
shbrief
▴ 20
1
vote
5
replies
2.6k
views
Preprocessing in scran::doubletCells
scran
simpleSingleCell
updated 6.5 years ago by
Aaron Lun
★ 29k • written 6.5 years ago by
Angelos Armen
• 0
4
votes
4
replies
3.8k
views
using limma and edgeR for differential expression of Drop-seq/10xGenomics scRNA-seq data
limma
edgeR
simplesingleCell
scran
scater
updated 6.6 years ago by
Aaron Lun
★ 29k • written 6.6 years ago by
Bogdan
▴ 670
4
votes
2
replies
2.5k
views
single cell data: adding random effects for subject with multilevel experiment design
SingleCellExperiment
scran
limma
simpleSingleCell
updated 6.7 years ago by
Aaron Lun
★ 29k • written 6.7 years ago by
Matt Mulé
• 0
1
vote
3
replies
2.3k
views
Troubles with barcodeRanks()
DropletUtils
SingleCellExperiement
DelayedArray
simpleSingleCell
updated 6.7 years ago by
Aaron Lun
★ 29k • written 6.7 years ago by
Steve Pederson
▴ 170
8
votes
2
replies
4.0k
views
Recommendations for combining multiple 10x runs into one SingleCellExperiment
singlecellexperiment
dropletutils
simplesinglecell
updated 7.3 years ago by
Aaron Lun
★ 29k • written 7.3 years ago by
Peter Hickey
▴ 760
0
votes
1
reply
1.3k
views
Unusual graph for Identifying HVGs from the normalized log-expression
workflows
simplesinglecell
updated 7.4 years ago by
Aaron Lun
★ 29k • written 7.4 years ago by
es.azadian
• 0
0
votes
1
reply
1.4k
views
The explanation of plotExplanatoryVariables plot
simplesinglecell
updated 7.9 years ago by
Aaron Lun
★ 29k • written 7.9 years ago by
Xingyu
• 0
1
vote
2
replies
2.4k
views
SingleCellExperiment object cannot be used by Scater package?
scater
singlecellexperiment
simplesinglecell
updated 8.0 years ago by
Aaron Lun
★ 29k • written 8.0 years ago by
Xingyu
• 0
3
votes
2
replies
4.4k
views
Sparse matrices in Bioconductor objects for single-cell analyses
scater
simpleSingleCell
workflows
updated 8.7 years ago by
Aaron Lun
★ 29k • written 8.7 years ago by
sandmann.t
▴ 70
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Answer: Should I use beta1 from limpa::dpc() or limpa::dpcCN(), if they differ?
by
Gordon Smyth
53k
I tend to use dpcCN(), because it more robust than dpc() to experiments with lots of samples and big differences between samples. You coul…
Answer: Which preprocessing steps are needed before running limpa?
by
Gordon Smyth
53k
Please see the example limpa analyses given at <http://github.com/smythlab/limpa>, especially the kidney cancer case study. limpa does not…
Comment: Why does IDTaxa trainingSet for SILVA contain "_2" suffixes in the taxonomy name
by
Korneel
• 0
Thanks for the quick reply! If I understand correctly, I think if I keep track of the full lineage, I shouldn't have any risk of collapsin…
Comment: Pathway abundance data from Curatedmetagenomics
by
yesquokkan
• 0
Hello, I was also wondering about the same issue. Did you figure it out?
Comment: [DESeq2] Changing dispersion estimates between runs of DESeq2
by
colinmccornack
• 0
FWIW: This seems to be due to architectural differences between two compute nodes I was running on, specifically with the DESeq2:::fitNbino…
Votes
Answer: Subsetting data verses contrasts [DESeq2]?
A: WGCNA differences two groups 10 replicates each group
A: WGCNA sample size minimum: why?
Comment: Why does IDTaxa trainingSet for SILVA contain "_2" suffixes in the taxonomy name
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