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simplesingleCell
•
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4
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5
replies
2.0k
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emptyDrops identified less non-empty cells with lower total UMI counts?
DropletUtils
scRNA-seq
emptyDrops
simplesinglecell
cell calling
updated 3.6 years ago by
Aaron Lun
★ 28k • written 4.7 years ago by
C T
▴ 140
4
votes
6
replies
1.2k
views
Feature selection across batches in simpleSingleCell when spike-ins are not available.
simpleSingleCell
updated 4.7 years ago by
Aaron Lun
★ 28k • written 4.7 years ago by
Angelos Armen
• 0
1
vote
1
reply
677
views
Feature selection across batches in simpleSingleCell "Correcting batch effects" vignette
simpleSingleCell
updated 4.7 years ago by
Aaron Lun
★ 28k • written 4.7 years ago by
Angelos Armen
• 0
5
votes
1
reply
4.5k
views
Error with DropletUtils::EmptyDrops()
DropletUtils
EmptyDrops
simpleSingleCell
updated 21 months ago by
Stefano Mangiola
▴ 10 • written 4.8 years ago by
shbrief
▴ 20
1
vote
5
replies
1.6k
views
Preprocessing in scran::doubletCells
scran
simpleSingleCell
updated 4.8 years ago by
Aaron Lun
★ 28k • written 4.8 years ago by
Angelos Armen
• 0
4
votes
4
replies
2.8k
views
using limma and edgeR for differential expression of Drop-seq/10xGenomics scRNA-seq data
limma
edgeR
simplesingleCell
scran
scater
updated 4.8 years ago by
Aaron Lun
★ 28k • written 4.8 years ago by
Bogdan
▴ 670
4
votes
2
replies
1.6k
views
single cell data: adding random effects for subject with multilevel experiment design
SingleCellExperiment
scran
limma
simpleSingleCell
updated 4.9 years ago by
Aaron Lun
★ 28k • written 4.9 years ago by
Matt Mulé
• 0
1
vote
3
replies
1.7k
views
Troubles with barcodeRanks()
DropletUtils
SingleCellExperiement
DelayedArray
simpleSingleCell
updated 5.0 years ago by
Aaron Lun
★ 28k • written 5.0 years ago by
Steve Pederson
▴ 170
7
votes
2
replies
2.4k
views
Recommendations for combining multiple 10x runs into one SingleCellExperiment
singlecellexperiment
dropletutils
simplesinglecell
updated 5.6 years ago by
Aaron Lun
★ 28k • written 5.6 years ago by
Peter Hickey
▴ 740
0
votes
1
reply
855
views
Unusual graph for Identifying HVGs from the normalized log-expression
workflows
simplesinglecell
updated 5.7 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
es.azadian
• 0
0
votes
1
reply
954
views
The explanation of plotExplanatoryVariables plot
simplesinglecell
updated 6.2 years ago by
Aaron Lun
★ 28k • written 6.2 years ago by
Xingyu
• 0
1
vote
2
replies
1.8k
views
SingleCellExperiment object cannot be used by Scater package?
scater
singlecellexperiment
simplesinglecell
updated 6.2 years ago by
Aaron Lun
★ 28k • written 6.2 years ago by
Xingyu
• 0
3
votes
2
replies
3.1k
views
Sparse matrices in Bioconductor objects for single-cell analyses
scater
simpleSingleCell
workflows
updated 6.9 years ago by
Aaron Lun
★ 28k • written 6.9 years ago by
sandmann.t
▴ 70
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Comment: ChAMP installation issue
by
e1271094
• 0
I apologise for any inconvenience. I have successfully installed the ChAMP package. However, I am now encountering a new error: myLoad <- …
Comment: How to use bootRanges to bootstrap small RNA loci (nullranges package)
by
Poonam
• 0
Yes, blockLength=200000, and seg=seg. I am concerned about the inter-feature distance of features. I generated this plot for various bloc…
Answer: Can I trust the differential expression analysis when the two groups overlap on
by
Michael Love
42k
Can you use `vst` instead of `rlog` and increase to 1000 or 2000 genes? I'm wondering if there's a way to characterize these 7 samples. …
Comment: Can I trust the differential expression analysis when the two groups overlap on
by
BioinfGuru
▴ 30
I think I am about to run into the same issue with my data, so I'll be interested to read peoples opinions on this also i.e. when the group…
Answer: How to approach time course RNAseq data? Seeing 80% DE genes in timepoint groups
by
Gordon Smyth
50k
The MDS plot shows that the time points are hugely different and whereas the replicate samples appear very consistent, with very low variat…
Votes
Comment: Store output of matchPWM()
Answer: Store output of matchPWM()
Answer: Store output of matchPWM()
Answer: ChipQC 'names' attribute [9] must be the same length as the vector [2]'
ChipQC 'names' attribute [9] must be the same length as the vector [2]'
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