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simplesingleCell
•
reset
4
votes
5
replies
2.6k
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emptyDrops identified less non-empty cells with lower total UMI counts?
DropletUtils
scRNA-seq
emptyDrops
simplesinglecell
cell calling
updated 4.5 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
C T
▴ 140
4
votes
6
replies
1.6k
views
Feature selection across batches in simpleSingleCell when spike-ins are not available.
simpleSingleCell
updated 5.7 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
Angelos Armen
• 0
1
vote
1
reply
903
views
Feature selection across batches in simpleSingleCell "Correcting batch effects" vignette
simpleSingleCell
updated 5.7 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
Angelos Armen
• 0
5
votes
1
reply
5.2k
views
Error with DropletUtils::EmptyDrops()
DropletUtils
EmptyDrops
simpleSingleCell
updated 2.7 years ago by
Stefano Mangiola
▴ 40 • written 5.7 years ago by
shbrief
▴ 20
1
vote
5
replies
2.1k
views
Preprocessing in scran::doubletCells
scran
simpleSingleCell
updated 5.7 years ago by
Aaron Lun
★ 28k • written 5.7 years ago by
Angelos Armen
• 0
4
votes
4
replies
3.4k
views
using limma and edgeR for differential expression of Drop-seq/10xGenomics scRNA-seq data
limma
edgeR
simplesingleCell
scran
scater
updated 5.8 years ago by
Aaron Lun
★ 28k • written 5.8 years ago by
Bogdan
▴ 670
4
votes
2
replies
2.1k
views
single cell data: adding random effects for subject with multilevel experiment design
SingleCellExperiment
scran
limma
simpleSingleCell
updated 5.9 years ago by
Aaron Lun
★ 28k • written 5.9 years ago by
Matt Mulé
• 0
1
vote
3
replies
2.0k
views
Troubles with barcodeRanks()
DropletUtils
SingleCellExperiement
DelayedArray
simpleSingleCell
updated 5.9 years ago by
Aaron Lun
★ 28k • written 5.9 years ago by
Steve Pederson
▴ 170
8
votes
2
replies
3.3k
views
Recommendations for combining multiple 10x runs into one SingleCellExperiment
singlecellexperiment
dropletutils
simplesinglecell
updated 6.5 years ago by
Aaron Lun
★ 28k • written 6.5 years ago by
Peter Hickey
▴ 750
0
votes
1
reply
1.1k
views
Unusual graph for Identifying HVGs from the normalized log-expression
workflows
simplesinglecell
updated 6.6 years ago by
Aaron Lun
★ 28k • written 6.6 years ago by
es.azadian
• 0
0
votes
1
reply
1.1k
views
The explanation of plotExplanatoryVariables plot
simplesinglecell
updated 7.1 years ago by
Aaron Lun
★ 28k • written 7.1 years ago by
Xingyu
• 0
1
vote
2
replies
2.1k
views
SingleCellExperiment object cannot be used by Scater package?
scater
singlecellexperiment
simplesinglecell
updated 7.2 years ago by
Aaron Lun
★ 28k • written 7.2 years ago by
Xingyu
• 0
3
votes
2
replies
3.9k
views
Sparse matrices in Bioconductor objects for single-cell analyses
scater
simpleSingleCell
workflows
updated 7.9 years ago by
Aaron Lun
★ 28k • written 7.9 years ago by
sandmann.t
▴ 70
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Comment: Bioconductor 3.20 Release Schedule
by
randyprice13102000
• 0
This offers much info https://bioconductor.org/checkResults/release/bioc-LATEST/
Comment: Filtering after DESeq
by
ATpoint
★ 4.8k
The problem is that your prefilter is not group-aware so the behaviour of seeing these sorts of outliers is expected. This is why I always …
Comment: GSEA preranked analysis downstream of DEseq2
by
ATpoint
★ 4.8k
All due respect, this is what people do since pvalues exist, so this paper is not a reference, not is this a special score that merits a na…
Comment: Check removeBatchEffect effectiveness
by
ATpoint
★ 4.8k
> insert size, mean GC, etc... This is not a classical "batch" in the common sense, at least I've never seen this to be corrected for expl…
Answer: Check removeBatchEffect effectiveness
by
Gordon Smyth
52k
Your call to removeBatchEffect() is not correct because that function does not have an argument called `batch3`, meaning that the batch fac…
Votes
Answer: DiffBind; Error: No sites have activity greater than filter value
DiffBind; Error: No sites have activity greater than filter value
remove X and Y chromosome genes in RNA-seq data using DESeq2 pipeline
How to remove X & Y chromosome genes from RNAseq data
Is it advisable to remove X and Y chromosome genes in mouse bulk RNA-seq data at the level of the count matrix?
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