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Showing :
DESeq2
•
reset
8
votes
9
replies
15k
views
GSEA preranked analysis downstream of DEseq2
deseq2
gsea
rnaseq
updated 7 hours ago by
Ajay
• 0 • written 8.7 years ago by
EJ
▴ 20
0
votes
0
replies
16
views
Filtering after DESeq
DESeq2
IHW
13 hours ago
caroline.zanchi
• 0
0
votes
3
replies
1.7k
views
Controlling for batch and estrus cycle using DESeq2 in RNA-seq analysis
deseq2
random effects
batch effects
RNA-seq
rna-seq
updated 21 hours ago by
donnycrimson
• 0 • written 7.8 years ago by
Dipro
• 0
0
votes
2
replies
2.6k
views
DESeq2 Design controlling for gender
deseq2
multiple factor design
updated 21 hours ago by
donnycrimson
• 0 • written 7.6 years ago by
rejane.troudet
• 0
0
votes
2
replies
439
views
Differing results with DESeq2
DESeq2
updated 1 day ago by
JKim
• 0 • written 21 days ago by
hampsdiv
• 0
0
votes
1
reply
205
views
Analyzing TnSeq data with DESeq2
DESeq2
updated 2 days ago by
cold.wolverine.arku
• 0 • written 2 days ago by
Lynn
• 0
0
votes
2
replies
298
views
RNA Seq using Lrt
DESeq2
3 days ago • updated 2 days ago
Rob
• 0
2
votes
3
replies
2.3k
views
How to convert transcript counts to gene counts in R?
DESeq2
tximport
updated 2 days ago by
ben
• 0 • written 2.5 years ago by
L
▴ 50
0
votes
1
reply
585
views
How do I set up my DESeq analysis when I know a few vehicle control samples provide background effects we don't want to see in any of the subsequent …
DESeq2
RUVSeq
updated 3 days ago by
Michael Love
43k • written 4 weeks ago by
Simran
• 0
0
votes
3
replies
1.7k
views
DeSeq Strategies for run to run Replication
DESeq2
Normalization
Clustering
replicate
updated 6 days ago by
Michael Love
43k • written 2.7 years ago by
Giorgio
• 0
0
votes
3
replies
714
views
Log2foldchange strange behavior
DESeq2
updated 11 days ago by
ATpoint
★ 4.8k • written 11 days ago by
RambakKulit
• 0
0
votes
1
reply
530
views
WGCNA
WGCNA
DESeq2
updated 14 days ago by
ATpoint
★ 4.8k • written 17 days ago by
bioinformaticssrm2011
• 0
0
votes
1
reply
826
views
Comparison of results obtained from a subset of the data versus the complete dataset for a few selected genes.
DESeq2
updated 14 days ago by
swbarnes2
★ 1.4k • written 15 days ago by
Bioinformatician_R
▴ 20
1
vote
1
reply
1.1k
views
PhD scholar
RNASeqAnalysis
DESeq2
updated 14 days ago by
shepherl
4.1k • written 15 days ago by
Richa
• 0
0
votes
2
replies
721
views
DESeq2 lfcShrink drastic shrinkage
DESeq2
updated 15 days ago by
Nick
• 0 • written 15 months ago by
theophile
• 0
1
vote
8
replies
2.3k
views
[ChIP-seq] DESeq2 on counts matrix from union set of called peaks
csaw
ChIPseq
DESeq2
DiffBind
6 weeks ago • updated 16 days ago
Luca
• 0
0
votes
1
reply
279
views
DESeq2 - am I extracting results incorrectly?
DESeq2
updated 17 days ago by
James W. MacDonald
68k • written 17 days ago by
bd2000
▴ 30
1
vote
3
replies
780
views
DESeq - help with pair wise comparisons
DESeq2
18 days ago
bd2000
▴ 30
1
vote
8
replies
2.5k
views
DESeq: Gene is not flagged as differentially expressed despite count data showing differences
DifferentialExpression
DESeq2
21 days ago • updated 18 days ago
ktz
• 0
0
votes
11
replies
1.9k
views
Question about using LRT test for time course experiments with DESeq2
DESeq2
updated 23 days ago by
Rob
• 0 • written 5 months ago by
Denise
• 0
5
votes
30
replies
5.9k
views
DESeq2 model - How to test case/control time course model when taking account within sample variability?
deseq2
multiple time points
time course
casecontrol
model design
updated 25 days ago by
wuwenhui2
• 0 • written 7.8 years ago by
heikki.sarin
▴ 10
0
votes
1
reply
562
views
DESeq2
org.Mmu.eg.db
DESeq2
TimeCourse
DMSOvsdrug
RNASeqData
updated 27 days ago by
ATpoint
★ 4.8k • written 28 days ago by
Cosmic
• 0
4
votes
6
replies
1.2k
views
Surprising behavior for DEXseq dispersion estimate plot?
DESeq2
DEXSeq
updated 28 days ago by
ATpoint
★ 4.8k • written 7 weeks ago by
Elijah
• 0
1
vote
4
replies
451
views
DESEQ2 looking at main effects while controlling for a continuous variable
DESeq2
5 weeks ago • updated 4 weeks ago
Lennie
• 0
1
vote
3
replies
5.7k
views
DESeq2 estimateSizeFactors with control genes
deseq2
differential gene expression
controls
references
updated 4 weeks ago by
sofia.rigou
• 0 • written 6.4 years ago by
cris.kgs
• 0
2
votes
4
replies
1.7k
views
deseq2 lfc shrinkage with few DEGs
RNASeq
DESeq2
updated 5 weeks ago by
Michael Love
43k • written 2.8 years ago by
Victor
▴ 10
38
votes
23
replies
17k
views
DESeq2 testing ratio of ratios (RIP-Seq, CLIP-Seq, ribosomal profiling)
deseq2
10.6 years ago • updated 5 weeks ago
Michael Love
43k
0
votes
4
replies
614
views
Guidance Needed: Best Practices for Handling Technical Replicates in RNA-seq Analysis
DESeq2
TechnicalReplicates
RNASeq
written 5 weeks ago by
questionanswer
• 0
0
votes
2
replies
465
views
Removing UTR from GTF for gene quantification
edgeR
DESeq2
updated 5 weeks ago by
Michael Love
43k • written 6 weeks ago by
rohitsatyam102
▴ 20
1
vote
1
reply
805
views
Use of org.Rn.eg.db with GRCr8 reference genome
DESeq2
org.Rn.eg.db
updated 6 weeks ago by
James W. MacDonald
68k • written 6 weeks ago by
Erika
• 0
1
vote
5
replies
4.1k
views
DESeq2 swap ensembl gene id rownames with gene symbol column
deseq2
dataframe
ensembl gene id
gene symbol
entrezid
updated 6 weeks ago by
Erika
• 0 • written 5.4 years ago by
jason.williams
▴ 10
1
vote
6
replies
1.9k
views
how to analysis htseq-count
deseq2
written 7.3 years ago by
Bioinformatics
▴ 30
1
vote
3
replies
4.9k
views
Log2 fold change value in DESeq2
deseq2
rnaseq
written 7.5 years ago by
AP
• 0
5
votes
3
replies
28k
views
DESeq2: thousands of NA for pvalues?
deseq2
rna-seq
DEGs
cooks cutoff
written 6.3 years ago by
aishu.jp
▴ 30
0
votes
1
reply
226
views
DESeq use in lipidomic analysis
DESeq2
package
Lipidomics
updated 7 weeks ago by
Michael Love
43k • written 7 weeks ago by
泓宇
• 0
0
votes
3
replies
673
views
how many significant figures are there in DESeq2 output
DESeq2
updated 7 weeks ago by
ATpoint
★ 4.8k • written 8 weeks ago by
Matthew McCormack
▴ 180
2
votes
6
replies
1.7k
views
Too many significant genes when integrating gtex and tcga
RNA-seq
DESeq2
updated 7 weeks ago by
Jane
• 0 • written 11 months ago by
Reza
• 0
2
votes
5
replies
1.0k
views
Group-specific condition effects with individuals nested: matrix is not full rank
DESeq2
8 weeks ago
picasa1983
• 0
3
votes
7
replies
2.1k
views
Vertical line at A=0, nothing else, on MA plot
deseq2
updated 8 weeks ago by
melissa
▴ 10 • written 6.1 years ago by
david.wheeler7
▴ 20
0
votes
1
reply
451
views
How to adjust for Cell Type Differences between groups in Pseudo-Bulk Differential Gene Expression analysis in scRNA-seq?
SingleCellData
DESeq2
pseudobulk
sing
updated 8 weeks ago by
James W. MacDonald
68k • written 8 weeks ago by
Sara
• 0
0
votes
3
replies
488
views
displaying the differential expression with deseq2 over 4X2 samples with the individual changes in gene expression per sample
DESeq2
updated 8 weeks ago by
Leonard
• 0 • written 11 weeks ago by
Martijn
• 0
2
votes
2
replies
608
views
Plot title for PCA in DESeq2
DESeq2
8 weeks ago
AL
• 0
1
vote
1
reply
503
views
Interpreting DESEQ2 interaction term and inclusion of covariate
DESeq2
updated 8 weeks ago by
swbarnes2
★ 1.4k • written 8 weeks ago by
Len
▴ 10
1
vote
1
reply
321
views
DEseq2 design for additive treatment
DESeq2
updated 8 weeks ago by
ATpoint
★ 4.8k • written 8 weeks ago by
picasa1983
• 0
0
votes
6
replies
2.0k
views
DESeq2 It is correct to use interaction term for patient variability?
deseq2
design
interaction term
R
written 7.2 years ago by
briverog
• 0
0
votes
3
replies
615
views
Differential variance analysis - can i use deseq2
DESeq2
DifferentialExpression
RNASeq
RNASeqData
scRNAseq
updated 9 weeks ago by
Michael Love
43k • written 9 weeks ago by
Marwan
• 0
3
votes
4
replies
2.3k
views
How to formulate 2 factor nested design in DESeq2
nested_design
RNASeqData
DESeq2
updated 9 weeks ago by
Mariane
• 0 • written 2.9 years ago by
JK Kim
▴ 20
1
vote
2
replies
1.1k
views
Weird p-values using DESeq2?
DESeq2
RNASeq
updated 10 weeks ago by
ATpoint
★ 4.8k • written 10 weeks ago by
ingrid.h
• 0
1
vote
5
replies
1.8k
views
p-value the same for different contrasts DESeq2
deseq2
design and contrast matrix
updated 10 weeks ago by
caroline.zanchi
• 0 • written 7.0 years ago by
chitsazanalex
▴ 10
0
votes
1
reply
194
views
Comparing Mock vs Infected in WT and Knockout Cells
DESeq2
DifferentialExpression
DifferentialRegulation
RNASeq
GeneSetEnrichment
updated 10 weeks ago by
Michael Love
43k • written 11 weeks ago by
Sahil
• 0
50 results • Page
1 of 1
Recent ...
Replies
Comment: GSEA preranked analysis downstream of DEseq2
by
Ajay
• 0
`logFC * -log10(pval)` seems to be the pi-score proposed in this [paper][1] [1]: https://pmc.ncbi.nlm.nih.gov/articles/PMC3957066/
Comment: error in ChAMP loading file
by
janwilseo
• 0
Any update on this post? I also had an issue with this. [erosion control](https://charlestonhydroseeding.com/)
Answer: CluserProfiler message "No gene can be mapped"
by
James W. MacDonald
68k
Two things. First, the order of your term2gene is backwards (should be term and then gene, like the name), and by default `enricher` adjust…
Comment: Controlling for batch and estrus cycle using DESeq2 in RNA-seq analysis
by
donnycrimson
• 0
One potential workaround might be to adjust for these factors using a combination of design matrices perhaps including batch as a covariate…
Comment: DESeq2 Design controlling for gender
by
donnycrimson
• 0
On a lighter note, while you are tackling such complex data, you might enjoy a quick break playing the [Slope Game](https://slopegame.lol)…
Votes
DiffBind; Error: No sites have activity greater than filter value
remove X and Y chromosome genes in RNA-seq data using DESeq2 pipeline
How to remove X & Y chromosome genes from RNAseq data
Is it advisable to remove X and Y chromosome genes in mouse bulk RNA-seq data at the level of the count matrix?
A: Unbalanced experiment with multiple samples from each patient.
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