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EdgeR
•
reset
1
vote
2
replies
1.2k
views
How to perform longitudinal analysis using EdgeR
StatisticalMethod
FoldChange
edgeR
RNASeq
22 months ago • updated 21 months ago
Sabiha
▴ 20
5
votes
2
replies
1.5k
views
Filter genes by expression for voomLmFit
DEG
edgeR
limma
voom
filterByExpr
updated 22 months ago by
Gordon Smyth
53k • written 22 months ago by
annikagable
• 0
1
vote
2
replies
1.4k
views
EdgeR: Exact test and glmQLFTest produce very different results
DifferentialExpression
edgeR
updated 22 months ago by
Gordon Smyth
53k • written 22 months ago by
Jurgen
• 0
12
votes
5
replies
4.9k
views
limma::voom vs edgeR::voomLmFit - when to use?
edgeR
limma
voom
RNASeq
muscat
updated 4 months ago by
Geoff
• 0 • written 22 months ago by
annikagable
• 0
0
votes
3
replies
1.2k
views
'Subtracting' counts on basis of Negative control media and water samples
limma
edgeR
RNASeq
DESeq2
22 months ago
Karthik
• 0
5
votes
2
replies
2.1k
views
DecideTest vs topTAGs in edgeR
RNAseq
StatisticalMethod
edgeR
updated 23 months ago by
Gordon Smyth
53k • written 23 months ago by
alvaroperona1
▴ 10
6
votes
7
replies
1.7k
views
[Bug] Segfault in edgeR::cpm
edgeR
segfault
updated 23 months ago by
ATpoint
★ 5.0k • written 24 months ago by
jonas.freimuth
▴ 20
0
votes
0
replies
701
views
Dual species RNA-seq, Library size and normalization
Normalization
edgeR
RNASeq
DESeq2
24 months ago
Florian
• 0
1
vote
3
replies
861
views
edgeR: Time course RNA-seq experiments
edgeR
updated 24 months ago by
ATpoint
★ 5.0k • written 2.0 years ago by
SuperDad
• 0
0
votes
1
reply
821
views
Batch effect adjust in model matrix
BatchEffect
edgeR
updated 2.0 years ago by
ATpoint
★ 5.0k • written 2.0 years ago by
Giuseppe
• 0
1
vote
6
replies
3.6k
views
Can I use EdgeR log2(cpm) instead of voom log2(cpm) to be analyzed as microarray data?
limma
edger
timecourse
without replicate
voom
written 8.9 years ago by
Camila Orellana
▴ 10
1
vote
4
replies
1.3k
views
MDS plot edgeR
edgeR
updated 2.1 years ago by
Gordon Smyth
53k • written 2.1 years ago by
jbono
▴ 10
3
votes
3
replies
954
views
comparing contrasts in edger/lima using makeContrasts function
DifferentialExpression
edgeR
limma
updated 2.1 years ago by
James W. MacDonald
68k • written 2.1 years ago by
stuartthafass
• 0
1
vote
2
replies
2.0k
views
Should I use CPM or Voom transformed values for downstream analysis?
edgeR
limma
2.1 years ago
petertam1343
• 0
1
vote
5
replies
1.2k
views
Why do I obtain Identical Adj.P value when running edgeR ?
edgeR
updated 2.1 years ago by
Gordon Smyth
53k • written 2.1 years ago by
Mohamed
▴ 30
3
votes
3
replies
1.1k
views
EdgeR and DESeq2, variance estimation and parametric test
parametrictest
edgeR
DESeq2
2.1 years ago
Nisha
• 0
2
votes
3
replies
1.5k
views
Import Salmon data with tximport and pool technical replicates
tximport
edgeR
written 2.2 years ago by
Vitor
• 0
1
vote
5
replies
1.2k
views
edgeR design/contrast matrix troubles
edgeR
updated 2.1 years ago by
James W. MacDonald
68k • written 2.1 years ago by
alex.hart
• 0
0
votes
1
reply
813
views
Is it possible to combine count gene expression data coming from one sequencing technology (RNA seq) with intensity gene expression data coming from …
DESeq2
RNASeqData
edgeR
limma
DifferentialExpression
updated 2.1 years ago by
ATpoint
★ 5.0k • written 2.1 years ago by
Sep
• 0
2
votes
4
replies
2.0k
views
Handle zero effective gene length when tximport RSEM results
tximport
edgeR
updated 2.1 years ago by
Michael Love
43k • written 2.1 years ago by
hanc
▴ 10
0
votes
0
replies
2.0k
views
Tutorial:
Read data file containing both counts and annotation into edgeR
rpkm
edgeR
featureCounts
2.1 years ago
Gordon Smyth
53k
2
votes
7
replies
5.2k
views
EdgeR glmLRT vs glmQLFTest
edgeR
DifferentialExpression
updated 2.1 years ago by
lucap
• 0 • written 4.5 years ago by
Marianna
▴ 20
0
votes
8
replies
3.4k
views
Question about filterByExpr function edgeR
edgeR
2.1 years ago
lucap
• 0
0
votes
2
replies
748
views
EdgeR FTest
edgeR
updated 2.2 years ago by
Gordon Smyth
53k • written 2.2 years ago by
nawaz.u.toyama
• 0
2
votes
5
replies
1.7k
views
Recommendation for large.n in filterByExpr
scRNAseq
edgeR
SingleCellExperiment
2.2 years ago
duez.tolga
• 0
0
votes
2
replies
1.3k
views
EdgeR and repeated measures: how to design a correct matrix having three time points
repeatedmeasure
edgeR
2.2 years ago
greta
• 0
1
vote
1
reply
651
views
A query about selecting the coefficient in glmLRT to test for by index
edgeR
updated 2.2 years ago by
Gordon Smyth
53k • written 2.2 years ago by
S
• 0
0
votes
9
replies
1.4k
views
estimateDisp() error
edgeR
updated 2.2 years ago by
Gordon Smyth
53k • written 2.2 years ago by
ASNbioinf
• 0
4
votes
3
replies
1.6k
views
Custom design for scRNA DGE with pseudobulking
scRNAseq
scrna
paireddesign
edgeR
pseudobulking
updated 2.2 years ago by
Gordon Smyth
53k • written 2.2 years ago by
garcia
• 0
4
votes
9
replies
3.6k
views
RNA seq differential expression analysis for large sample size
edgeR
DESeq2
2.2 years ago
Luca
• 0
2
votes
5
replies
2.0k
views
Question about RNAseq analysis in EdgeR to identify common and donor specific differentially expressed genes
R
edgeR
RNASeq
2.2 years ago
mohammedtoufiq91
▴ 10
6
votes
2
replies
1.2k
views
How to access the C++ code of the function .cxx_ave_log_cpm in the aveLogCPM.default function of EdgeR package?
edgeR
2.2 years ago
Josué
▴ 20
1
vote
1
reply
934
views
NA p values in edgeR results
DifferentialExpression
edgeR
updated 2.3 years ago by
Gordon Smyth
53k • written 2.3 years ago by
miona.rankovic
• 0
1
vote
2
replies
1.1k
views
edgeR, RRBS Library size preparation
RRBSdata
edgeR
methylationArrayAnalysis
2.3 years ago
Krzysztof
• 0
1
vote
1
reply
780
views
Best way to handle batch effects from difference Mice?
RNASeqData
BatchEffect
edgeR
limma
updated 2.3 years ago by
Gordon Smyth
53k • written 2.3 years ago by
Sara
• 0
3
votes
2
replies
1.1k
views
Does this RNAseq experiment require a repeated measures approach?
StatisticalMethod
RNASeqData
edgeR
DESeq2
updated 2.3 years ago by
Gordon Smyth
53k • written 2.3 years ago by
CDSPARKS
▴ 30
0
votes
5
replies
1.4k
views
Model and Contrasts with 4 variables
contrast
edgeR
DESeq2
updated 2.3 years ago by
James W. MacDonald
68k • written 2.3 years ago by
Cristina
• 0
3
votes
6
replies
1.5k
views
Question on constructing design matrix and defining contrasts for analysis
wgcna
DESeq2
edgeR
limma
design
updated 2.4 years ago by
Gordon Smyth
53k • written 2.4 years ago by
mohammedtoufiq91
▴ 10
2
votes
1
reply
858
views
DESeq2 or edgeR for Presence and Absence of Proteins
edgeR
DESeq2
updated 2.4 years ago by
ATpoint
★ 5.0k • written 2.4 years ago by
calvincornell1996
• 0
5
votes
11
replies
7.3k
views
Visualization of negative log CPM values
Visualization
Normalization
ggplot2
edgeR
written 2.4 years ago by
Sabiha
▴ 20
5
votes
7
replies
2.2k
views
Question about setting threshold using filtering by expression on genes in edgeR
filtering
RNA-Seq
CPM
edgeR
filterByExpr
updated 2.4 years ago by
Gordon Smyth
53k • written 2.4 years ago by
mohammedtoufiq91
▴ 10
0
votes
1
reply
933
views
DESeq2 about comparasion of gene expression change on two-time points between two group samples.
DESeq2
RNASeqData
edgeR
limma
DifferentialExpression
updated 2.4 years ago by
swbarnes2
★ 1.4k • written 2.4 years ago by
simon
• 0
0
votes
1
reply
853
views
CI for logCPM (fitted values)
RNAseq
edgeR
updated 2.4 years ago by
Gordon Smyth
53k • written 2.4 years ago by
Lina
▴ 10
3
votes
4
replies
1.1k
views
Anova table edgeR limma
limma
RNASeq
edgeR
2.4 years ago
KATHERINE CHUERE NUNES
• 0
1
vote
2
replies
1.0k
views
Obtention of the binomial transformed count table with edgeR
DESeq2
edgeR
RNASeq
DifferentialExpression
updated 2.4 years ago by
Gordon Smyth
53k • written 2.4 years ago by
Sofía
• 0
1
vote
3
replies
1.5k
views
Is it okay to increase filtering to get more significant Adjusted P Values for DEGs found using Limma?
RNASeqPower
edgeR
DifferentialExpression
limma
RNASeq
updated 2.4 years ago by
Gordon Smyth
53k • written 2.4 years ago by
Sara
• 0
0
votes
1
reply
1.1k
views
Subtype specific differential expression analysis
R
limmaGUI
edgeR
limma
regutools
updated 2.4 years ago by
Gordon Smyth
53k • written 2.4 years ago by
qiz218591
• 0
2
votes
1
reply
992
views
edgeR with Successive Differences Contrast Coding?
hypothesisTesting
contrastCoding
edger
ExperimentalDesign
expressionDifferences
updated 2.5 years ago by
Gordon Smyth
53k • written 2.5 years ago by
Hannes
• 0
0
votes
3
replies
1.3k
views
edgeR DEGs multilevel
edgeR
RNASeq
DifferentialRegulation
updated 2.5 years ago by
Gordon Smyth
53k • written 2.5 years ago by
bioinfoWanderer
• 0
1
vote
1
reply
695
views
Using R to measure differential gene expression of one gene due to different treatments using RNASeq counts
DESeq2
edgeR
RNAseq
updated 2.5 years ago by
Gordon Smyth
53k • written 2.5 years ago by
cmareevicars
• 0
2,031 results • Page
3 of 41
Recent ...
Replies
Comment: Help with defining groups
by
Phinney, Brett
▴ 10
I got it working , thank you!!!
Comment: Check removeBatchEffect effectiveness
by
massimo
• 0
I would kindly ask for additional support. When not including the design_timepoint argument in removeBatchEffect and thus keeping the inter…
Comment: Error Loading Experiment from SimBenchData Package
by
ksankaran
• 0
Aha, that was it! The object was class Seurat, but I didn't have it installed. After installing, everything works as expected.
Comment: Streamlining the computing time for MiloDE p-value correction in large dataset?
by
ATpoint
★ 5.0k
Is a method that is so computationally expensive really beneficial and necessary? You seem to have biological replication, don't you, so ps…
Answer: NA values for DESeq2 p-values and adjusted p-values using large sample sizes
by
ATpoint
★ 5.0k
Outliers without replacement, independent filtering or all-zero counts for that gene. That's the three options that trigger NAs https://bio…
Votes
Comment: Check removeBatchEffect effectiveness
Gene expression associated with continuous (quantitative) traits (Limma/edgeR/correlation)
Answer: Error Loading Experiment from SimBenchData Package
Comment: incomplete imputation of missing values with LEA impute() function
Answer: Expected memory usage for analyzing large single-cell seq datasets (> 450,000 ce
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