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rnaseq123
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224
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Assistance with Bulk RNA-seq Data Analysis Including Random Effect (`duplicateCorrelation` from `limma`)
limma
RNAseq123
10 weeks ago
tim.meese
• 0
1
vote
2
replies
451
views
Improving the design matrices vignette in RNAseq123
RNAseq123
7 months ago
Lluís Revilla Sancho
▴ 760
1
vote
1
reply
638
views
Differential gene expression analysis
RNASeqData
RNAseq123
updated 12 months ago by
ATpoint
★ 4.6k • written 12 months ago by
kaurprab
• 0
0
votes
2
replies
822
views
Deseq2 paird analysis in a cohort mixed of paired and unpaired samples
DESeq2
RNAseq123
22 months ago
fatemeh.peimani
• 0
0
votes
3
replies
1.2k
views
DESeq2 Statistic - Padj NA
DESeq2
RNAseq123
rnaseq
deseq2
metatranscriptomic
updated 23 months ago by
Michael Love
43k • written 24 months ago by
irish.prg
• 0
1
vote
6
replies
4.6k
views
KEGG enrichment analysis for non model organism
RNAseq123
2.0 years ago
afsanarupa1
• 0
2
votes
5
replies
1.3k
views
Can we massively improve RNA-Seq results by slightly reducing the counts in it?
edgeR
RNAseq123
updated 2.0 years ago by
Gordon Smyth
52k • written 2.1 years ago by
Istvan Albert
▴ 50
0
votes
1
reply
2.0k
views
Confused about understanding the output and statistics of BAM file after STAR aligning
RNAseq123
VariantFiltering
Alignment
VariantAnnotation
updated 2.1 years ago by
James W. MacDonald
67k • written 2.1 years ago by
Mohamed
▴ 30
1
vote
1
reply
931
views
Confused about design for RNA seq experiment
RNAseq
ExperimentData
RNAseq123
RNA
ExperimentalDesign
updated 2.2 years ago by
ATpoint
★ 4.6k • written 2.2 years ago by
Mohamed
▴ 30
0
votes
0
replies
717
views
Plotting a line plot for genes across 4-species
RNAseq123
2.4 years ago
Mohamed
▴ 30
2
votes
1
reply
1.3k
views
Error in RNAseq123 analysis, unexpected symbol.
RNAseq123
Rna
updated 2.5 years ago by
Gordon Smyth
52k • written 2.5 years ago by
Mikołaj Mierzejewski
• 0
5
votes
6
replies
3.3k
views
Large difference in PCA plot based on LCPM (log counts per million) and VST (variance stabilizing transformations)
PCAtools
edgeR
pcaExplorer
RNASeq
RNAseq123
2.7 years ago
Alan
• 0
0
votes
1
reply
984
views
python and R
snpeff
python
tophat
r
RNAseq123
updated 3.4 years ago by
Kevin Blighe
★ 4.0k • written 3.4 years ago by
kusum
• 0
1
vote
1
reply
1.8k
views
featureCounts running too long
RNAseq123
updated 10 months ago by
difraiadomenico
• 0 • written 3.8 years ago by
demirkalecy
▴ 10
0
votes
0
replies
664
views
Circular RNA
CircularRNA
RNASeq
RNAseq123
3.8 years ago
rpokhre2
• 0
1
vote
3
replies
5.3k
views
Using TMM normalized counts in Deseq2
RNAseq123
written 3.9 years ago by
mmicky5050
• 0
12
votes
4
replies
4.8k
views
differential expression analysis of htseq data with edgeR/voom
voom
htseq
edgeR
limma
RNASeq123
updated 5.9 years ago by
Gordon Smyth
52k • written 7.5 years ago by
wd
▴ 30
17 results • Page
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Comment: Feature Counts - potential bug with outfile.jcounts
by
chris2.a.white
• 0
Hi Gordon, Thank you for pointing out that FeatureCounts detects junction reads de novo. It is not in the manual, and maybe could be. Give…
Answer: Pre-filtering TPM kallisto for ssGSEA / DESeq2
by
Michael Love
43k
I've seen in some papers ~1 TPM for saying something in mouse/human etc. was "minimally expressed". For an average length gene, 10 count…
Answer: Feature Counts - potential bug with outfile.jcounts
by
Gordon Smyth
52k
The behavior that you have documented is expected and is explained in the featureCounts documentation. The first thing to understand is th…
Answer: Feature Counts -R XS Read Assignment Tag
by
Gordon Smyth
52k
No, featureCounts cannot change the XS tag to a different tag, because that would change the information in the output. featureCounts needs…
Comment: Margins for gene_set_enrichment_plot
by
Leonardo Collado Torres
★ 1.1k
Thanks! This one might be worth remaking with `ComplexHeatmap`. Similar to https://github.com/LieberInstitute/spatialLIBD/pull/91 that was …
Votes
Answer: Mapping Between GenBank ID's and Uniprot
Comment: What type of counts data to import for performing Isoform analysis in edgeR
A: Relations used in GO.db GOBPANCESTOR
Relations used in GO.db GOBPANCESTOR
Answer: RSubread output significantly different from bash Subread output
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