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ApeGLM
•
reset
1
vote
2
replies
298
views
Questions in Regards to LFC shrinkage in DESeq2
Dseq2
RNASeqData
apeglm
12 weeks ago
Gordon
• 0
2
votes
10
replies
845
views
How to run allelic imbalance in apeglm
apeglm
6 months ago • updated 5 months ago
euge.eugenio
▴ 20
0
votes
1
reply
730
views
lfcShrink error: the line search routine failed
apeglm
DESeq2
updated 8 months ago by
Michael Love
42k • written 8 months ago by
Yuqin
• 0
0
votes
2
replies
1.4k
views
Error: insufficient numeric precision during Log fold change shrinkage with apeglm
apeglm
updated 14 months ago by
Michael Love
42k • written 15 months ago by
Juan Pablo
• 0
0
votes
4
replies
920
views
lfc direction of effect flipped for ashr, apeGLM, but accurate to biological data for non-shrunken
DESeq2
apeglm
DESeq
ApeGLM
Ashr
updated 16 months ago by
Michael Love
42k • written 16 months ago by
Ndimensional
▴ 20
2
votes
7
replies
2.4k
views
optimHess problem in apeglm/DESeq2
DESeq2
apeglm
updated 18 months ago by
Michael Love
42k • written 18 months ago by
ATpoint
★ 4.5k
3
votes
2
replies
1.1k
views
DESeq2 lfcShrink: swapping reference gives different results for the same comparison
DESeq2
apeglm
20 months ago • updated 19 months ago
Daphnia
• 0
1
vote
5
replies
2.3k
views
DESeq2 outlier options and LFC Shrinkage
DESeq2
RNASeq
RnaSeqSampleSize
apeglm
updated 2.3 years ago by
Michael Love
42k • written 2.3 years ago by
Riley
• 0
1
vote
3
replies
1.2k
views
LFC shrinkage setting negative LFC svalues to zero
DESeq2
DifferentialExpression
apeglm
updated 2.4 years ago by
Michael Love
42k • written 2.4 years ago by
81das_col
• 0
1
vote
2
replies
1.1k
views
running apeglm on a normal model and multiple coefficients
shrinkage
apeglm
2.6 years ago
Frederik Ziebell
▴ 30
1
vote
2
replies
1.4k
views
Use of shrunken LFCs to address questions about the distribution of LFCs between gene categories.
deseq2
apeglm
DESeq2
2.9 years ago
i.sudbery
▴ 40
2
votes
3
replies
2.1k
views
Choosing an svalue threshold from apeglm shrunken results in DESeq2
apeglm
DESeq2
updated 3.2 years ago by
Michael Love
42k • written 3.2 years ago by
i.sudbery
▴ 40
1
vote
5
replies
1.7k
views
lfcShrink probelm in many 0 count genes RNA-seq data
ashr
DESeq2
apeglm
updated 3.2 years ago by
Michael Love
42k • written 3.3 years ago by
Guandong Shang
▴ 40
0
votes
1
reply
853
views
Non-significant but high LFC after apeglm shrinkage
DESeq2
apeglm
updated 3.7 years ago by
Michael Love
42k • written 3.7 years ago by
hpapoli
• 0
0
votes
1
reply
1.3k
views
Plotting the most expressed genes after conducting DESeq2
DESeq2
pheatmap
apeglm
updated 3.8 years ago by
Michael Love
42k • written 3.8 years ago by
amoaristotle
▴ 10
2
votes
2
replies
1.3k
views
DESeq2 in R
DESeq2
apeglm
updated 3.8 years ago by
swbarnes2
★ 1.4k • written 3.8 years ago by
amoaristotle
▴ 10
0
votes
2
replies
1.4k
views
DSEQ apeglm
DESeq2
apeglm
3.8 years ago
amoaristotle
▴ 10
2
votes
1
reply
1.4k
views
apeglm -- influence of reference level on logFC and svalues
DESeq2
apeglm
updated 3.8 years ago by
Michael Love
42k • written 3.8 years ago by
ATpoint
★ 4.5k
0
votes
3
replies
1.2k
views
Using altHypothesis on apleglm-shrunken log2FoldChanges
apeglm
DESeq2
3.9 years ago
zefrieira
• 0
0
votes
1
reply
1.2k
views
DESeq2 - shrunken LFC calculation
apeglm
DESeq2
updated 3.9 years ago by
Michael Love
42k • written 3.9 years ago by
alexandr.gopanenko
▴ 50
0
votes
3
replies
827
views
Influence of DEG in ASE analysis within apeglm
apeglm
updated 4.1 years ago by
Michael Love
42k • written 4.1 years ago by
Vincent.C.
• 0
1
vote
1
reply
771
views
Exceeding Over-dispersion parameter bbEstDisp
apeglm
bbEstdisp
allele ratio
updated 4.2 years ago by
Michael Love
42k • written 4.2 years ago by
Vincent.C.
• 0
5
votes
10
replies
1.5k
views
Allelic imbalance in apeglm
apeglm
Allelic imbalance
concept
updated 4.2 years ago by
Michael Love
42k • written 4.2 years ago by
Vincent.C.
• 0
1
vote
4
replies
2.0k
views
Using different LFC shrinkage estimators for spike-in normalised RNA-seq data
deseq2
apeglm
rna-seq
spike-in
updated 4.3 years ago by
Michael Love
42k • written 4.3 years ago by
nadezda.fursova
▴ 10
3
votes
2
replies
2.3k
views
lfcShrink function in DEseq2 and s-values
deseq2
apeglm
updated 4.3 years ago by
Michael Love
42k • written 4.3 years ago by
josmantorres
▴ 10
2
votes
1
reply
829
views
What are the steps to use a complex model of Allelic imbalance through apeglm
apeglm
complex model
ASE
Allelic imbalance
updated 4.3 years ago by
Michael Love
42k • written 4.3 years ago by
Vincent.C.
• 0
1
vote
1
reply
1.0k
views
Why s-values instead of p-values with non-default lfcThreshold and apeglm shrinkage in DESeq2
deseq2
apeglm
updated 4.4 years ago by
Michael Love
42k • written 4.4 years ago by
jabaron.phd
• 0
3
votes
4
replies
2.5k
views
Reporting S values? Reporting padj values?
deseq2
apeglm
updated 4.5 years ago by
Michael Love
42k • written 4.5 years ago by
n.tear
▴ 20
4
votes
4
replies
3.0k
views
RNA seq analysis using Deseq2 and apeglm lfc shrinkage
deseq2
fdrtool
apeglm
updated 4.6 years ago by
Michael Love
42k • written 4.6 years ago by
Paul
• 0
0
votes
2
replies
1.1k
views
Coefficient names for multiple group comparisons to use with lfcShrink & apeglm
deseq2
lfcShrink
apeglm
updated 4.9 years ago by
Michael Love
42k • written 4.9 years ago by
coulsonr
▴ 20
0
votes
2
replies
1.5k
views
lfcShrink in DESeq2
deseq2
apeglm
updated 5.3 years ago by
Michael Love
42k • written 5.3 years ago by
Meghdad
• 0
3
votes
3
replies
1.7k
views
Using alternative LFC shrinkage methods for computing p-values in DESeq2
deseq2
apeglm
statistics
updated 5.5 years ago by
Michael Love
42k • written 5.5 years ago by
zefrieira
• 0
3
votes
2
replies
1.7k
views
Calculate S/N ratios from apeglm for GSEA
apeglm
fgsea
Signal-To-Noise Ratio
updated 5.5 years ago by
Michael Love
42k • written 5.5 years ago by
coulsonr
▴ 20
2
votes
1
reply
1.1k
views
Deactivate svalue=T in lfcShrink - DESeq2 - apeglm
deseq2
apeglm
updated 5.6 years ago by
Michael Love
42k • written 5.6 years ago by
ATpoint
★ 4.5k
2
votes
4
replies
1.5k
views
What is the biological meaning of genes with FC = 0 after shrinkage
deseq2
apeglm
updated 5.7 years ago by
Michael Love
42k • written 5.7 years ago by
polyptwo
• 0
0
votes
2
replies
3.1k
views
DESeq2 coefficients for APEGLM-shrinkage
deseq2
apeglm
updated 6.0 years ago by
Michael Love
42k • written 6.0 years ago by
zolotaryovgl
• 0
4
votes
4
replies
2.4k
views
Unexpected MAplot after apeglm shrinkage in DESeq2
apeglm
deseq2
rna-seq
updated 6.1 years ago by
Michael Love
42k • written 6.1 years ago by
ATpoint
★ 4.5k
4
votes
2
replies
2.8k
views
Interpreting the results from lfcShrink() with apeglm in DESeq2
deseq2
apeglm
lfcshrink
updated 6.1 years ago by
Michael Love
42k • written 6.1 years ago by
charles.foster
▴ 160
1
vote
4
replies
1.8k
views
Log2 fold-change shrinkage fails with lfcShrink when using the 'apeglm' method
deseq2
apeglm
lfcshrink
6.3 years ago
coulsonr
▴ 20
0
votes
4
replies
1.4k
views
Error in Apeglm Method for Effect Size Shrinkage
deseq2
apeglm
updated 6.4 years ago by
Michael Love
42k • written 6.4 years ago by
rbenel
▴ 40
10
votes
6
replies
6.3k
views
Log2FoldChange shrinkage method in DESeq2
deseq2
apeglm
lfcshrink
updated 6.5 years ago by
Michael Love
42k • written 6.5 years ago by
Benjamin Ostendorf
▴ 90
41 results • Page
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Replies
Comment: ANOVA like approach of edgeR
by
Yunshun Chen
▴ 870
If you do it this way, the dispersion estimates would be much higher than they should (as the cell type difference is not accounted for in …
Comment: ANOVA like approach of edgeR
by
SamGG
▴ 350
Simply remove the cell type from the model. design <- model.matrix(~targets$Status)
Answer: ANOVA like approach of edgeR
by
Yunshun Chen
▴ 870
You could try the followings: > design <- model.matrix(~ 0 + group) > contrast <- makeContrasts(PvsL = 0.5*(L.pregnant + B.pregnan…
Answer: Identical samples after deseq2 batch effect removal
by
maripane
• 0
Thanks Michael, so is there a way to work around this issue? Can I somehow make sure I can remove this batch effects without overcorrecti…
Comment: CleanUpRNAseq with a GTF that is missing mitochondria annotation data
by
rcreed
• 0
The updated version worked well on my data. Thank you!
Votes
A: ANOVA-like test with edgeR
Answer: CleanUpRNAseq with a GTF that is missing mitochondria annotation data
Answer: CleanUpRNAseq with a GTF that is missing mitochondria annotation data
A: TopGO: How to retrieve the gene list related to a GO ID ?
A: Error in DESeqDataSet : some values in assay are not integers
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