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DESeq2
•
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0
votes
0
replies
33
views
LRT, pairwise comparisons and p-value adjustment
DESeq2
17 hours ago
boczniak767
▴ 720
0
votes
3
replies
79
views
Counter-directional LFC shrinkage
DESeq2
updated 10 hours ago by
swbarnes2
★ 1.4k • written 21 hours ago by
ennui
• 0
0
votes
2
replies
112
views
How to add Ensembl ids after Pseudobulk analysis by DESeq2
Seurat
DESeq2
single-cell
scRNA
Pseudobulk
updated 1 day ago by
Guido Hooiveld
★ 4.0k • written 3 days ago by
Sara
• 0
0
votes
3
replies
140
views
GSVA function
DESeq2
GSVA
ssGSEA
updated 2 days ago by
Robert Castelo
★ 3.3k • written 4 days ago by
michelafrancesconi8
• 0
0
votes
2
replies
113
views
Normalisation using clinical traits in RNA seq using DESeq2
DESeq2
clinicaltraits
Normalization
batchnormalisation
5 days ago • updated 2 days ago
sukeshinik5
• 0
0
votes
4
replies
240
views
Volcano plot of scRNA-seq data not "centered"
DESeq2
updated 3 days ago by
ATpoint
★ 4.2k • written 5 days ago by
tjl
• 0
1
vote
8
replies
199
views
Slightly different count plots from R and spreadsheet
DESeq2
ggplot2
6 days ago
boczniak767
▴ 720
1
vote
3
replies
178
views
LRT, more than two conditions and pairwise comparisons
DESeq2
updated 8 days ago by
James W. MacDonald
66k • written 9 days ago by
boczniak767
▴ 720
2
votes
4
replies
257
views
DESeq2- Mysterious interaction suppresses DEGs
DESeq2
PCAplot
RNA-Seq
DesignModel
updated 8 days ago by
Michael Love
42k • written 11 days ago by
shaunpeterson
• 0
0
votes
6
replies
632
views
Numerical differences in DESeq2 output depending on different design matrix construction
DESeq2
12 days ago • updated 8 days ago
Ken C
• 0
4
votes
9
replies
338
views
Can DESeq2 rlog normalized matrix reflects time course result directly?
DESeq2
timecoursedata
GeneExpression
updated 12 days ago by
ATpoint
★ 4.2k • written 14 days ago by
pangtaihin
• 0
0
votes
3
replies
173
views
Assymetric MA plot with no overall trend
DESeq2
DifferentialExpression
updated 14 days ago by
Michael Love
42k • written 14 days ago by
Kasper
• 0
1
vote
2
replies
287
views
How can I create a eigencorplot of pca created from deseq2 object with PCAtools
DESeq2
eigencorplot
PCAtools
15 days ago • updated 12 days ago
BioinfGuru
▴ 30
0
votes
8
replies
588
views
Can I trust the differential expression analysis when the two groups overlap on the PCA?
DESeq2
pcaExplorer
updated 14 days ago by
BioinfGuru
▴ 30 • written 15 days ago by
Eric Katagirya
• 0
0
votes
1
reply
131
views
normalization across different species for DESeq2 analysis
DESeq2
normalization
Normalization
acrossspecies
updated 14 days ago by
Michael Love
42k • written 15 days ago by
piemmea
• 0
0
votes
1
reply
205
views
Differential gene expression analysis results
DESeq2
DifferentialExpression
updated 19 days ago by
ATpoint
★ 4.2k • written 19 days ago by
sajadahmad41454
• 0
0
votes
1
reply
127
views
normalization using Deseq2
DESeq2
rnaseqGene
Normalization
updated 20 days ago by
ATpoint
★ 4.2k • written 20 days ago by
sajadahmad41454
• 0
2
votes
7
replies
484
views
deseq2 results
DESeq2
DifferentialExpression
rnaseqGene
DifferentialRegulation
21 days ago • updated 18 days ago
sajadahmad41454
• 0
2
votes
3
replies
284
views
Too many significant genes when integrating gtex and tcga
RNA-seq
DESeq2
updated 24 days ago by
ATpoint
★ 4.2k • written 25 days ago by
Reza
• 0
1
vote
1
reply
203
views
Handling multiple differential expression comparisons
DESeq2
updated 24 days ago by
Michael Love
42k • written 25 days ago by
Zainab
▴ 10
0
votes
6
replies
325
views
Once again a "Model matrix not full rank"
DESeq2
design
coldata
updated 25 days ago by
swbarnes2
★ 1.4k • written 26 days ago by
arfranco
▴ 130
0
votes
2
replies
188
views
DESeq2 v1.35.0
DESeq2
updated 26 days ago by
Michael Love
42k • written 26 days ago by
Espresso
• 0
0
votes
2
replies
227
views
Deseq2 and Normalization of RNA sequencing data
DESeq2
27 days ago • updated 26 days ago
kcarey
• 0
0
votes
3
replies
252
views
Differences in differential analysis methods for RNA-Seq
DESeq2
StatisticalMethod
Proteomics
DifferentialExpression
RNASeq
updated 27 days ago by
ATpoint
★ 4.2k • written 27 days ago by
james.zhang20
• 0
1
vote
3
replies
338
views
Getting Error in hclust(d, method = method): NA/NaN/Inf in foreign function call when trying to subset pheatmap
DESeq2
updated 21 days ago by
ATpoint
★ 4.2k • written 27 days ago by
angelathuynh5
• 0
0
votes
3
replies
315
views
log2FoldChange value is way too different when compared with counts(dds)
DESeq2
updated 27 days ago by
Michael Love
42k • written 28 days ago by
HAK
• 0
1
vote
1
reply
166
views
Fold change calculation in Diffbind vs. DESEQ2?
DiffBind
ChIPSeqData
DESeq2
updated 28 days ago by
Michael Love
42k • written 28 days ago by
marcusn
• 0
0
votes
1
reply
145
views
putting my own expression data in deseq2
DESeq2
updated 29 days ago by
ATpoint
★ 4.2k • written 29 days ago by
Dev
• 0
0
votes
2
replies
184
views
Timecourse RNASeq analysis
DESeq2
ImpulseDE2
timecour
timecoursedata
moanin
29 days ago • updated 26 days ago
Aurora
• 0
0
votes
1
reply
144
views
Padj "NA" with thousands of low count although the raw read counts are quite large
DESeq2
updated 28 days ago by
Michael Love
42k • written 29 days ago by
Agung
• 0
1
vote
4
replies
545
views
DEseq2 coefficient
DESeq2
coefficient
3 months ago • updated 27 days ago
JKim
• 0
0
votes
2
replies
1.6k
views
How to use spike-in information (sequences from another species) with DESeq2::DESeq()
DESeq2
SpikeIn
Normalization
updated 19 days ago by
maria.soler
• 0 • written 16 months ago by
kalavattam
▴ 10
6
votes
19
replies
2.6k
views
DESeq2 a lot of genes showing up as differentially expressed that only have 1 sample with any expression
deseq2
updated 28 days ago by
Sey R.
• 0 • written 4.7 years ago by
hsbio
▴ 10
33 results • Page
1 of 1
Recent ...
Replies
Answer: Counter-directional LFC shrinkage
by
swbarnes2
★ 1.4k
The y axes in those plots are logged. The average for condition1 is going to be higher than condition2 because of the very high outliers. …
Comment: Cannot install oligo on Fedora
by
Guido Hooiveld
★ 4.0k
Thanks for all suggestions and getting to the bottom of this! Unfortunately, fixing C code goes way above my skill level, so I cannot contr…
Comment: Cannot install oligo on Fedora
by
James W. MacDonald
66k
I think the solution is for the C code in `oligo` to be updated to work with more modern versions of `zlib`. The `zlibbioc` package is real…
Comment: Error in gseDO function
by
Guido Hooiveld
★ 4.0k
I don't fully understand what you are asking... Yet, in my opinion it doesn't make sense to use the results of only 12 genes for ORA (nor G…
Comment: Counter-directional LFC shrinkage
by
ATpoint
★ 4.2k
Is this RNA-seq? The unshrunken MA-plot looks very odd and indicates that normalization is completely offset.
Votes
A: DESeq2: add annotations (from data frame) to DESeqDataSet
Answer: Error in gseDO function
Comment: what the test method for enrichGO in clusterProfiler?
A: Comparison between two different group's pathway analysis results
DiffBind Batch Effect
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