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SingleCellData
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News:
Course: Single-cell analysis with R/Bioconductor
SingleCellExperiment
SingleCell
SingleCellWorkflow
SingleCellData
5 months ago
Ludwig Geistlinger
▴ 80
1
vote
3
replies
1.3k
views
Possible issue following the logNormCounts(filtered) with resulted fit
single-cell
SingleCell
SingleCellExperiment
SingleCellWorkflow
SingleCellData
8 months ago
Konstantinos Yeles
▴ 90
0
votes
1
reply
1.6k
views
How to adjust for Cell Type Differences between groups in Pseudo-Bulk Differential Gene Expression analysis in scRNA-seq?
SingleCellData
DESeq2
pseudobulk
sing
updated 11 months ago by
James W. MacDonald
68k • written 11 months ago by
Sara
• 0
0
votes
0
replies
456
views
Help with mouse cell type annotation
annotation
SingleR
mouse
SingleCellData
scRNAseq
12 months ago
Muhammad
• 0
0
votes
2
replies
1.6k
views
Integration of Single Cell Experiments with batchelor::fastMNN
fastMNN
SingleCellData
BatchEffect
batchelor
16 months ago • updated 15 months ago
José
• 0
0
votes
1
reply
782
views
Is CEMiTool for gene coexpression analysis suitable for single cell RNA Seq data?
SingleCellData
CEMiTool
updated 18 months ago by
James W. MacDonald
68k • written 18 months ago by
Menna
• 0
0
votes
1
reply
836
views
Dimensionality reduction visualization
imcRtools
SingleCellData
imcdatasets
SpatialWorkflow
updated 2.1 years ago by
James W. MacDonald
68k • written 2.1 years ago by
Simon
• 0
0
votes
10
replies
4.0k
views
Easiest way to convert read10xMolInfo data into a dataframe in R with gene labels as row names
tidyverse
R
SingleCellExperiment
SingleCellData
written 2.2 years ago by
Sitapriya
• 0
0
votes
2
replies
1.1k
views
Fail to annotate chromosome data to single cell sequencing data obtained from cellranger
AnnotationHubSoftware
SingleCellData
annotationTools
updated 2.2 years ago by
ATpoint
★ 5.0k • written 2.2 years ago by
yw24418
• 0
0
votes
3
replies
1.5k
views
insufficient unique points for computing knee/inflection points
DropletUtils
SingleCellData
updated 2.5 years ago by
ATpoint
★ 5.0k • written 2.5 years ago by
p.vlachou-efstathiou
• 0
0
votes
2
replies
2.2k
views
Error in SingleR, no common genes between 'test' and 'ref'
singleR
SingleCellExperiment
SingleCellData
2.6 years ago
Matvii Mykhailichenko
• 0
1
vote
0
replies
1.2k
views
Job:
Research Assistant - Computational Biology/Immunology, Memorial Sloan Kettering, New York
SingleCellData
ComputationalBiology
2.9 years ago
Chrysothemis
▴ 10
0
votes
0
replies
1.4k
views
Job:
Job as a bioinformatician in Hamburg, Germany
DimensionReduction
SingleCellData
multiomics
MachineLearning
3.2 years ago
Diego's
• 0
0
votes
0
replies
1.0k
views
Gene Id duplicated
SingleCell
SingleCellData
updated 3.4 years ago by
ATpoint
★ 5.0k • written 3.4 years ago by
Romina
• 0
2
votes
4
replies
2.2k
views
How to detect LacZ tagged cells from single cell
SingleCell
SingleCellExperiment
SingleCellWorkflow
SingleCellData
updated 6 months ago by
Partly
• 0 • written 3.4 years ago by
k1m
▴ 20
2
votes
4
replies
1.8k
views
Summary of library size factor is all equal to one
SingleCell
SingleCellData
SingleCellWorkflow
SingleCellExperiment
3.4 years ago
Romina
• 0
0
votes
1
reply
1.5k
views
Splatter (unknown correlation between gene expression level in different cell types)
SingleCellData
splatter
updated 3.8 years ago by
luke.zappia
▴ 50 • written 3.8 years ago by
Fatima
• 0
0
votes
1
reply
1.3k
views
Running PLAGE gets killed
GSVA
SingleCellData
updated 3.9 years ago by
Robert Castelo
★ 3.4k • written 3.9 years ago by
Amna
• 0
2
votes
1
reply
2.9k
views
Convert AnnotatedDataFrame S4 object from GEO datasets to Seurat Object
biobase
SingleCellData
seurat
SingleCell
updated 4.1 years ago by
James W. MacDonald
68k • written 4.1 years ago by
NGS_enthusiast
• 0
1
vote
3
replies
2.7k
views
10X FeatureBarcode producing too many "cells"
SingleCellExperiment
DropletUtils
SingleCellData
updated 5.1 years ago by
Aaron Lun
★ 29k • written 5.1 years ago by
msn
▴ 10
20 results • Page
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Replies
Comment: Which preprocessing steps are needed before running limpa?
by
miro165sabo
• 0
Hi @gordonsmyth, thank you a lot for very fast and valuable response. I edited the question to provide more details.
Answer: Should I use beta1 from limpa::dpc() or limpa::dpcCN(), if they differ?
by
Gordon Smyth
53k
I tend to use dpcCN(), because it more robust than dpc() to experiments with lots of samples and big differences between samples. You coul…
Answer: Which preprocessing steps are needed before running limpa?
by
Gordon Smyth
53k
Please see the example limpa analyses given at <http://github.com/smythlab/limpa>, especially the kidney cancer case study. limpa does not…
Comment: Why does IDTaxa trainingSet for SILVA contain "_2" suffixes in the taxonomy name
by
Korneel
• 0
Thanks for the quick reply! If I understand correctly, I think if I keep track of the full lineage, I shouldn't have any risk of collapsin…
Comment: Pathway abundance data from Curatedmetagenomics
by
yesquokkan
• 0
Hello, I was also wondering about the same issue. Did you figure it out?
Votes
Answer: Which preprocessing steps are needed before running limpa?
Answer: Should I use beta1 from limpa::dpc() or limpa::dpcCN(), if they differ?
Answer: Which preprocessing steps are needed before running limpa?
Answer: Which preprocessing steps are needed before running limpa?
Answer: Should I use beta1 from limpa::dpc() or limpa::dpcCN(), if they differ?
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