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Showing :
contrasts
•
reset
3
votes
3
replies
629
views
DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
DESeq2
contrasts
updated 8 months ago by
ATpoint
★ 4.6k • written 8 months ago by
bioinf
▴ 10
2
votes
3
replies
632
views
edgeR, 3 x 2 design with a batch effect and contrasts
design
edgeR
contrasts
updated 9 months ago by
Gordon Smyth
52k • written 9 months ago by
JKim
• 0
0
votes
3
replies
1.1k
views
Multiple Testing When Using Multiple Contrasts/Comparisons in DESEQ2
MultipleComparison
DESeq2
Genetics
contrasts
RNASeq
updated 18 months ago by
James W. MacDonald
67k • written 18 months ago by
a26fd14e
• 0
1
vote
2
replies
749
views
Individual DESeq2 runs versus a single DESeq2 run with contrasts produce different results
contrasts
DESeq2
19 months ago
jovel_juan
▴ 30
3
votes
6
replies
1.4k
views
Question about making Contrasts for comparisons and assignment of values
edgeR
contrasts
limma
design
model.matrix
2.1 years ago
mohammedtoufiq91
▴ 10
1
vote
3
replies
869
views
Limma F-statistic identical for different comparison, why?
limma
F-statistic
contrasts
updated 2.2 years ago by
Gordon Smyth
52k • written 2.2 years ago by
jeltje.van.baren
• 0
3
votes
8
replies
4.4k
views
DESeq2 how to specify contrast to test difference of differences
DifferentialExpression
contrasts
interaction
DESeq2
written 2.7 years ago by
Nicole
▴ 10
0
votes
3
replies
1.8k
views
limma voom contrasts
contrasts
limma
voom
updated 2.8 years ago by
Gordon Smyth
52k • written 2.8 years ago by
S T
▴ 90
0
votes
1
reply
911
views
DESeq2 2x3 design contrast question
DESeq2
contrasts
updated 2.9 years ago by
Michael Love
43k • written 2.9 years ago by
Mir
• 0
0
votes
2
replies
1.2k
views
limma voom; fitted lines, factors that aren't of interest, and hypothesis testing
topTable
voom
contrasts
3.4 years ago
James
• 0
0
votes
3
replies
1.3k
views
DESeq2: difficulty with contrasts and main effects
DESeq2
phyloseq
contrasts
updated 3.4 years ago by
Kevin Blighe
★ 4.0k • written 3.4 years ago by
Mir
• 0
2
votes
8
replies
2.5k
views
DESeq2 numbers of DEGs not consistent with PCA plot
DifferentialExpression
DESeq2
contrasts
3.6 years ago
Jane
▴ 10
3
votes
8
replies
1.5k
views
Same contrasts returning different edgeR results
edger
limma
contrasts
DGE
updated 5.1 years ago by
James W. MacDonald
67k • written 5.1 years ago by
s.connell
• 0
0
votes
1
reply
1.2k
views
DESeq2 - how to run contrasts leaving out one factor, not just using the reference?
deseq2
contrasts
reference level
updated 5.1 years ago by
Michael Love
43k • written 5.1 years ago by
ananda2
• 0
0
votes
0
replies
848
views
EdgeR: Transforming coefficients before testing for differential expression
edger
contrasts
model.matrix
circadian
5.1 years ago
ingerslev
• 0
0
votes
1
reply
1.3k
views
DESeq2 very complex design - how extract contrasts of interest?
DESeq2
RNA-Seq
Multifactorial Design
GLM
Contrasts
updated 5.1 years ago by
Michael Love
43k • written 5.1 years ago by
ananda2
• 0
2
votes
3
replies
1.6k
views
DESeq2 2x2 factorial design: find genes unique to factor A?
deseq2
deseq
differential expression
contrasts
updated 5.1 years ago by
Michael Love
43k • written 5.1 years ago by
Keith Hughitt
▴ 180
0
votes
4
replies
1.2k
views
Setting up contrasts with 'limma', patient data, small number of repeats
limma
nanostring
contrasts
updated 5.3 years ago by
Gordon Smyth
52k • written 5.3 years ago by
uridavid.akavia
• 0
0
votes
1
reply
835
views
DESeq2 compairson of 3 samples from 1 type
deseq2
multiple factor design
contrasts
experimental design
updated 5.6 years ago by
Michael Love
43k • written 5.6 years ago by
kehoe
• 0
3
votes
5
replies
1.7k
views
Experimental design RNAseq differential expression
edger
limma
design
contrasts
batch
updated 5.8 years ago by
Aaron Lun
★ 28k • written 5.8 years ago by
Pietro
▴ 40
2
votes
6
replies
1.4k
views
Making sense out of makeContrasts and using coefficents for contrasts
edger
contrasts
updated 5.9 years ago by
Gordon Smyth
52k • written 5.9 years ago by
Maiv
• 0
21 results • Page
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Recent ...
Replies
Comment: How do I fix an odd voom plot in a combined dataset?
by
1sunmic2
• 0
Thanks for responding! I did change the code slightly: dge1 <- DGEList(counts = combined_data_counts) keep <- rowSums(cpm(dge1)…
Comment: Incorporating Salmon offset matrix into Wilcoxon DEG analysis
by
Andre
• 0
The estimateGLMRobustDisp() function seems to have an effect on glmQLFit(), even in edgeR 4 (4.4.1). If I use estimateGLMRobustDisp(), like…
Comment: Incorporating Salmon offset matrix into Wilcoxon DEG analysis
by
Gordon Smyth
52k
Here, estimateGLMRobustDisp() was used just to get the diagnostic BCV plot by plotBCV(y). The robust NB dispersions were not used for the D…
Answer: Please, I desperately need help with DESeq2. Three groups of repeated measures w
by
Michael Love
43k
I have to reserve my time on the support site for software related questions. I recommend working with a local statistician or someone fami…
Answer: Is it possible to work with DESeq2 where data is not properly matched for sex a
by
Michael Love
43k
I would probably just do the M vs M analysis. You could instead include a term for sex and have a model with all 45 samples, but the M vs M…
Votes
Answer: How do I fix an odd voom plot in a combined dataset?
Answer: How do I fix an odd voom plot in a combined dataset?
Comment: How do I fix an odd voom plot in a combined dataset?
C: How does edgeR cpm function calculate log(CPM) values?
C: How does edgeR cpm function calculate log(CPM) values?
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