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Showing :
Contrasts
•
reset
3
votes
3
replies
1.1k
views
DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
DESeq2
contrasts
updated 17 months ago by
ATpoint
★ 5.0k • written 17 months ago by
bioinf
▴ 10
2
votes
3
replies
1.0k
views
edgeR, 3 x 2 design with a batch effect and contrasts
design
edgeR
contrasts
updated 18 months ago by
Gordon Smyth
53k • written 18 months ago by
JKim
• 0
0
votes
3
replies
1.5k
views
Multiple Testing When Using Multiple Contrasts/Comparisons in DESEQ2
MultipleComparison
DESeq2
Genetics
contrasts
RNASeq
updated 2.2 years ago by
James W. MacDonald
68k • written 2.3 years ago by
a26fd14e
• 0
1
vote
2
replies
889
views
Individual DESeq2 runs versus a single DESeq2 run with contrasts produce different results
contrasts
DESeq2
2.3 years ago
jovel_juan
▴ 30
3
votes
6
replies
1.8k
views
Question about making Contrasts for comparisons and assignment of values
edgeR
contrasts
limma
design
model.matrix
2.8 years ago
mohammedtoufiq91
▴ 10
1
vote
3
replies
1.1k
views
Limma F-statistic identical for different comparison, why?
limma
F-statistic
contrasts
updated 2.9 years ago by
Gordon Smyth
53k • written 2.9 years ago by
jeltje.van.baren
• 0
3
votes
8
replies
5.2k
views
DESeq2 how to specify contrast to test difference of differences
DifferentialExpression
contrasts
interaction
DESeq2
written 3.4 years ago by
Nicole
▴ 10
0
votes
3
replies
2.1k
views
limma voom contrasts
contrasts
limma
voom
updated 3.6 years ago by
Gordon Smyth
53k • written 3.6 years ago by
S T
▴ 90
0
votes
1
reply
1.1k
views
DESeq2 2x3 design contrast question
DESeq2
contrasts
updated 3.7 years ago by
Michael Love
43k • written 3.7 years ago by
Mir
• 0
0
votes
2
replies
1.4k
views
limma voom; fitted lines, factors that aren't of interest, and hypothesis testing
topTable
voom
contrasts
4.1 years ago
James
• 0
0
votes
3
replies
1.6k
views
DESeq2: difficulty with contrasts and main effects
DESeq2
phyloseq
contrasts
updated 4.2 years ago by
Kevin Blighe
★ 4.0k • written 4.2 years ago by
Mir
• 0
2
votes
8
replies
2.9k
views
DESeq2 numbers of DEGs not consistent with PCA plot
DifferentialExpression
DESeq2
contrasts
4.4 years ago
Jane
▴ 10
3
votes
8
replies
1.8k
views
Same contrasts returning different edgeR results
edger
limma
contrasts
DGE
updated 5.8 years ago by
James W. MacDonald
68k • written 5.8 years ago by
s.connell
• 0
0
votes
1
reply
1.4k
views
DESeq2 - how to run contrasts leaving out one factor, not just using the reference?
deseq2
contrasts
reference level
updated 5.8 years ago by
Michael Love
43k • written 5.8 years ago by
ananda2
• 0
0
votes
0
replies
976
views
EdgeR: Transforming coefficients before testing for differential expression
edger
contrasts
model.matrix
circadian
5.8 years ago
ingerslev
• 0
0
votes
1
reply
1.5k
views
DESeq2 very complex design - how extract contrasts of interest?
DESeq2
RNA-Seq
Multifactorial Design
GLM
Contrasts
updated 5.9 years ago by
Michael Love
43k • written 5.9 years ago by
ananda2
• 0
2
votes
3
replies
1.9k
views
DESeq2 2x2 factorial design: find genes unique to factor A?
deseq2
deseq
differential expression
contrasts
updated 5.9 years ago by
Michael Love
43k • written 5.9 years ago by
Keith Hughitt
▴ 180
0
votes
4
replies
1.4k
views
Setting up contrasts with 'limma', patient data, small number of repeats
limma
nanostring
contrasts
updated 6.0 years ago by
Gordon Smyth
53k • written 6.0 years ago by
uridavid.akavia
• 0
0
votes
1
reply
975
views
DESeq2 compairson of 3 samples from 1 type
deseq2
multiple factor design
contrasts
experimental design
updated 6.4 years ago by
Michael Love
43k • written 6.4 years ago by
kehoe
• 0
3
votes
5
replies
2.0k
views
Experimental design RNAseq differential expression
edger
limma
design
contrasts
batch
updated 6.6 years ago by
Aaron Lun
★ 29k • written 6.6 years ago by
Pietro
▴ 40
2
votes
6
replies
1.6k
views
Making sense out of makeContrasts and using coefficents for contrasts
edger
contrasts
updated 6.6 years ago by
Gordon Smyth
53k • written 6.6 years ago by
Maiv
• 0
21 results • Page
1 of 1
Recent ...
Replies
Comment: Help with defining groups
by
Phinney, Brett
▴ 10
I got it working , thank you!!!
Comment: Check removeBatchEffect effectiveness
by
massimo
• 0
I would kindly ask for additional support. When not including the design_timepoint argument in removeBatchEffect and thus keeping the inter…
Comment: Error Loading Experiment from SimBenchData Package
by
ksankaran
• 0
Aha, that was it! The object was class Seurat, but I didn't have it installed. After installing, everything works as expected.
Comment: Streamlining the computing time for MiloDE p-value correction in large dataset?
by
ATpoint
★ 5.0k
Is a method that is so computationally expensive really beneficial and necessary? You seem to have biological replication, don't you, so ps…
Answer: NA values for DESeq2 p-values and adjusted p-values using large sample sizes
by
ATpoint
★ 5.0k
Outliers without replacement, independent filtering or all-zero counts for that gene. That's the three options that trigger NAs https://bio…
Votes
Comment: Check removeBatchEffect effectiveness
Gene expression associated with continuous (quantitative) traits (Limma/edgeR/correlation)
Answer: Error Loading Experiment from SimBenchData Package
Comment: incomplete imputation of missing values with LEA impute() function
Answer: Expected memory usage for analyzing large single-cell seq datasets (> 450,000 ce
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