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DeSeq2
•
reset
0
votes
3
replies
2.3k
views
How can i fix this problem in R
S4Vectors
DESeq2
updated 3.4 years ago by
ATpoint
★ 5.0k • written 3.4 years ago by
Habib
• 0
0
votes
1
reply
1.8k
views
Error: DESeq(dds , useT = TRUE, minmu = 1e-06, test = "LRT", : likelihood ratio test requires a 'reduced' design, see ?DESeq
scRNAseq
DESeq2
updated 3.4 years ago by
ATpoint
★ 5.0k • written 3.4 years ago by
abdi.zhr26
• 0
9
votes
9
replies
4.5k
views
Using Age or other numerical variable to model expression in deseq2
DESeq2
updated 3.4 years ago by
James W. MacDonald
68k • written 3.4 years ago by
chm
• 0
0
votes
4
replies
1.8k
views
I got error message about DESeqDataSetFromMatrix
DESeq2
updated 3.4 years ago by
swbarnes2
★ 1.4k • written 3.4 years ago by
Md. Faridul
• 0
2
votes
6
replies
2.1k
views
DESeq2: relevel() unsuccessfully setting control group
DESeq2
updated 3.4 years ago by
Slot
• 0 • written 4.7 years ago by
vanbelj
▴ 30
1
vote
3
replies
3.1k
views
miRNA differential expression analysis - treat as transcript level?
tximport
DESeq2
edgeR
swish
miRNA
updated 3.4 years ago by
Michael Love
43k • written 3.5 years ago by
BG
• 0
2
votes
8
replies
2.6k
views
RSEM - tximport; where is effective_length information used? Implications for scRNA?
RSEM
tximport
scRNAseq
DESeq2
3.4 years ago
Kelen
▴ 10
4
votes
3
replies
3.3k
views
Large difference between lfcshrink and non-shrunk DESeq2 results
DESeq2
3.5 years ago • updated 3.4 years ago
Dave
▴ 10
0
votes
20
replies
3.5k
views
Different DESeq2 results (both FC and pvalue) by changing col/row orders in input
DESeq2
3.5 years ago • updated 3.4 years ago
Weisheng
• 0
2
votes
4
replies
2.3k
views
deseq2 lfc shrinkage with few DEGs
RNASeq
DESeq2
updated 8 months ago by
Michael Love
43k • written 3.5 years ago by
Victor
▴ 10
3
votes
3
replies
1.4k
views
Nested analysis or Collapsed-replicate analysis?
RNASeq
DESeq2
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
giulia.lopatriello
• 0
0
votes
1
reply
778
views
Interpretation of comparison results in nested designs
DESeq2
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
thkapell
▴ 10
1
vote
3
replies
2.0k
views
Error in shrinkage for DESeq2
sva
DESeq2
3.5 years ago
Kent
• 0
1
vote
3
replies
1.3k
views
Obtaining many more significant differentially expressed genes by subsetting than just using contrasts
DESeq2
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
Tom
▴ 10
0
votes
1
reply
720
views
Is my design bad for accounting for Batch Effect in DESEQ2?
DESeq2
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
Stephan
• 0
1
vote
1
reply
827
views
roughDispEstimate and momentsDispEstimate
estimator
roughmethodofmoments
DESeq2
R
Code
updated 3.5 years ago by
Basti
▴ 780 • written 3.5 years ago by
stew
• 0
0
votes
5
replies
1.4k
views
problem withDESeq2
DESeq2
updated 3.5 years ago by
andres.wokaty
▴ 90 • written 3.5 years ago by
fnu.chelsea
• 0
1
vote
5
replies
1.8k
views
Error in DESeqDataSetFromMatrix
DESeq2
3.5 years ago
fnu.chelsea
• 0
0
votes
1
reply
978
views
lfc Shrinkage with multiple comparison groups
RNAseq
lfcShrinkage
DESeq2
updated 3.5 years ago by
ATpoint
★ 5.0k • written 3.5 years ago by
m.lorgen.06
• 0
0
votes
1
reply
769
views
Multifactor analysis
DESeq2
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
Manmohit
• 0
1
vote
3
replies
875
views
DESeq2 changing Interaction term interpretation
DESeq2
written 3.5 years ago by
mmartinez
• 0
0
votes
1
reply
780
views
Nested analysis or Collapsed-replicate analysis?
RNASeq
DESeq2
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
giulia.lopatriello
• 0
0
votes
2
replies
1.1k
views
Design set up, interaction or group? Two Salinities treatments, Five Populations, Three Replicates
Design
DESeq2
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
lgspeight
• 0
1
vote
1
reply
1.1k
views
RNA-Seq normalization and batch effect removal
RNASeq
BatchEffect
DESeq2
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
Alexandre
• 0
1
vote
3
replies
1.1k
views
summary function when applying lfcThreshold gives a different result than when operating on the result of the same
DESeq2
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
JaneDoe
• 0
2
votes
5
replies
1.3k
views
DE genes with zero counts
DESeq2
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
pt
• 0
0
votes
3
replies
1.4k
views
Controversial DESEQ2 results
phyloseq
DESeq2
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
Stephan
• 0
1
vote
6
replies
1.5k
views
DESeq2 counts error in as.data.frame
DESeq2
updated 3.5 years ago by
jeroen.gilis
▴ 100 • written 3.5 years ago by
ali reza
• 0
2
votes
2
replies
3.4k
views
Create TPM gene tables/dataframe from ReCount3
recount3
TCGA
GTEx
DESeq2
3.5 years ago
David
▴ 20
6
votes
7
replies
3.3k
views
Batch effect with one sample being in two batches
BatchEffect
DESeq2
3.5 years ago • updated 3.4 years ago
lmrogers34
▴ 10
0
votes
3
replies
3.6k
views
ENSEMBL to gene symbol
biomaRt
DESeq2
updated 3.5 years ago by
Guido Hooiveld
★ 4.1k • written 3.5 years ago by
Matt
• 0
4
votes
3
replies
3.1k
views
Log2FoldChange and normalized count values are not consistent?
Normalization
DESeq2
3.5 years ago
Karla
▴ 10
1
vote
7
replies
2.5k
views
RUVseq, DESeq2, and different codesets for nanostring data
RUVSeq
DESeq2
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
xiaofeiwang18266
▴ 60
0
votes
1
reply
1.1k
views
Aggregate differentially expressed genes across all contrast results using DEVis
DifferentialExpression
NanoporeRNASeq
DESeq2
GeneExpression
DEVis
updated 3.5 years ago by
James W. MacDonald
68k • written 3.5 years ago by
Ana
• 0
3
votes
4
replies
8.7k
views
Dispersion plot -Interpretation -DESeq2
DESeq2
3.5 years ago
suhanya
▴ 20
0
votes
5
replies
3.6k
views
GO enrichment with non-model organism, supplying GO mappings
clusterProfiler
goseq
topGO
DESeq2
updated 3.5 years ago by
James W. MacDonald
68k • written 3.5 years ago by
Lucía
▴ 30
5
votes
4
replies
2.6k
views
How to formulate 2 factor nested design in DESeq2
nested_design
RNASeqData
DESeq2
updated 9 months ago by
Mariane
• 0 • written 3.5 years ago by
JK Kim
▴ 30
0
votes
2
replies
3.6k
views
Deseq2 Time series - 2 treatments 1 control 6 Time Points
timecourse
DESeq2
updated 3.5 years ago by
jeroen.gilis
▴ 100 • written 3.5 years ago by
Maria
• 0
3
votes
8
replies
5.4k
views
DESeq2 how to specify contrast to test difference of differences
DifferentialExpression
contrasts
interaction
DESeq2
written 3.6 years ago by
Nicole
▴ 10
1
vote
1
reply
1.1k
views
paired analysis : return data matrix with indvidual/subject effects eliminated.
RUVSeq
sva
DESeq2
updated 3.5 years ago by
James W. MacDonald
68k • written 3.5 years ago by
ramonfallon
▴ 20
0
votes
1
reply
904
views
Creating DESeq object and choosing a contrast
DESeq2
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
Dev Raj
• 0
3
votes
3
replies
1.3k
views
Merging two plated in R
dese
DESeq2
updated 3.5 years ago by
James W. MacDonald
68k • written 3.5 years ago by
lmrogers34
▴ 10
3
votes
9
replies
3.3k
views
Warnings when using "ashr" in lfcshrinkage for DESeq2
RNASeq
DESeq2
updated 2.9 years ago by
ayabalbaa1990
• 0 • written 3.5 years ago by
andrebolerbarros
▴ 20
2
votes
4
replies
3.9k
views
ashr vs apeglm for interaction data
lfcShrink
DESeq2
3.5 years ago
Ali Barry
▴ 40
1
vote
4
replies
1.3k
views
different results in DESeq2 when comparing groups in two ways
DESeq2
3.5 years ago
zohreh.fazelan
• 0
2
votes
1
reply
2.3k
views
Difference between raw counts and featureCounts for DESeq2
deseq
featurecounts
counts
DESeq2
updated 3.5 years ago by
swbarnes2
★ 1.4k • written 3.5 years ago by
Hicham
▴ 10
1
vote
2
replies
1.1k
views
DESeq2 paired design: Differential list comparing fold changes
DESeq2
3.5 years ago • updated 3.4 years ago
athira
• 0
3
votes
4
replies
2.1k
views
DESeq2: Extracting Averaged Counts Information from dds and rld
DESeq2
3.6 years ago • updated 3.5 years ago
vanbelj
▴ 30
0
votes
6
replies
1.9k
views
DESeq2, Calculate the size factor by using median-of-ratio
median
DESeq2
3.6 years ago • updated 3.5 years ago
KEREN
• 0
0
votes
1
reply
3.3k
views
The results(dds) function in DESeq2 gives Na values for padj
DESeq2
updated 3.6 years ago by
ATpoint
★ 5.0k • written 3.6 years ago by
keshav.prasad.gubbi
• 0
4,840 results • Page
21 of 97
Recent ...
Replies
Answer: problem reading bam files into nucleR
by
James W. MacDonald
68k
The first step is to update to the current versions of R/Bioconductor and try again. It's possible that you have run into a bug that's alre…
Answer: Question about DESeq2 Design for Paired Treatment Study
by
ATpoint
★ 5.0k
It's a paired analysis so you don't need any patient-specific covariates such as age and sex. What you call batch effect might just be the …
Comment: problem reading bam files into nucleR
by
efoss
▴ 10
I thought I might be able to track down a problematic line in the bam file by repeatedly splitting the bam file in and then running the co…
Answer: two questions about limma
by
Gordon Smyth
53k
Answered here: https://support.bioconductor.org/p/16318/
Comment: Highly similar RNA-seq samples in PCA - pooling or technical duplication?
by
Ahmed Salah
• 0
Thank you very much! will take that into consideration
Votes
Upcoming Ensembl API and data access changes - new blog post available
Comment: Highly similar RNA-seq samples in PCA - pooling or technical duplication?
Answer: Large logFC but somewhat high FDR
Comment: upstream git branches of bioc-release
Comment: upstream git branches of bioc-release
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