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LIMMA
•
reset
0
votes
2
replies
119
views
Cross-validation with multiple control subgroups in limma
limma
multtest
1 day ago • updated 14 hours ago
Ali Barry
▴ 40
1
vote
9
replies
609
views
Normalizing batch effects using technical replicates for DGE analysis between two conditions from 2 different runs
limma
DESeq2
edgeR
updated 1 hour ago by
yinhjk602
• 0 • written 4 days ago by
anna.cot.anna.cot
▴ 30
0
votes
2
replies
256
views
Advice needed: Using limma for differential pathway analysis with continuous disease progression scores
limma
DifferentialExpression
Designmatrix
12 days ago • updated 11 days ago
Tadeoye
▴ 20
0
votes
2
replies
427
views
sva: get a sv for RIN scores
limma
sva
variancePartition
voom
RNASeq
updated 15 days ago by
Gordon Smyth
52k • written 16 days ago by
Lina
▴ 10
2
votes
3
replies
1.1k
views
How to annotate a limma Elist raw class object with an annotation file
limma
AnnotationDbi
updated 17 days ago by
James W. MacDonald
67k • written 17 days ago by
giulia.gentile
▴ 10
1
vote
2
replies
292
views
contrast matrix design for a continuous variable
limma
19 days ago
dtzhu337
• 0
3
votes
3
replies
562
views
Crossover design with limma
limma
crossover
updated 5 days ago by
Gordon Smyth
52k • written 24 days ago by
Marek Gierlinski
▴ 30
0
votes
9
replies
790
views
Differential Expression Genes as Independent Variables
limma
DifferentialExpression
variancePartition
dream
updated 17 days ago by
James W. MacDonald
67k • written 22 days ago by
abadgerw
• 0
6
votes
10
replies
2.4k
views
What type of counts data to import for performing Isoform analysis in edgeR
limma
isoform
edgeR
salmon
R
updated 13 days ago by
ATpoint
★ 4.6k • written 24 days ago by
mohammedtoufiq91
▴ 10
1
vote
3
replies
395
views
Struggle with the replicates of epic array data
limma
epicarray
IlluminaHumanMethylationEPICanno.ilm10b2.hg19
written 29 days ago by
Melisa Tecik
• 0
0
votes
2
replies
178
views
List of DEGs belonging to an Agilent-single color microarray experiment using Limma package
limma
4 weeks ago
giulia.gentile
▴ 10
4
votes
2
replies
528
views
Using voom-transformed counts for unsupervised analyses (PCA, Random Forest, Elastic Net): Best practices?
limma
limma-voom
updated 4 weeks ago by
Gordon Smyth
52k • written 4 weeks ago by
Ahdee
▴ 50
0
votes
2
replies
173
views
Limma Interaction Effect with Covariates
limma
DMRcate
4 weeks ago
kitzhu
• 0
2
votes
3
replies
876
views
[How to construct a model.matrix ]
limma
modelmatrix
updated 5 weeks ago by
Gordon Smyth
52k • written 5 weeks ago by
kyj2226
• 0
3
votes
4
replies
623
views
Limma- Print-tip loess normalisation-printer layout information does not match M row dimension
Normalization
limma
within-arraynormalisation
Printerlayout
5 weeks ago
dseauer
• 0
1
vote
3
replies
424
views
makeContrastsDream for Interaction
limma
DifferentialExpression
variancePartition
dream
6 weeks ago • updated 24 days ago
abadgerw
• 0
0
votes
5
replies
619
views
Merge Replicates vs Mixed Model
limma
DifferentialExpression
variancePartition
dream
6 weeks ago • updated 20 days ago
abadgerw
• 0
1
vote
8
replies
1.1k
views
Combining RNAseq and microarray data using edgeR/limma-voom
limma
voom
RNASeq
edgeR
Microarray
written 6 weeks ago by
Adam Marstrand
• 0
1
vote
2
replies
933
views
Consistent Decimals In Data After Antibody Array Background Correction Using Limma:nec Function
limma
MicroarrayData
Normalization
6 weeks ago
Ai
• 0
3
votes
3
replies
891
views
Robust way of dealing with low number of samples for Differential Gene Expression
limma
edgeR
DESeq2
MAST
written 8 weeks ago by
Satoshi
• 0
4
votes
3
replies
800
views
Analysis of 2*4 factorial design
limma
updated 7 weeks ago by
Gordon Smyth
52k • written 8 weeks ago by
Sudipta
• 0
2
votes
12
replies
1.6k
views
limma technical repeat correction
limma
lmer
duplicateCorrelation
technicalrepeats
8 weeks ago • updated 4 weeks ago
john
• 0
4
votes
6
replies
1.5k
views
LIMMA Differing P-Values for Treatment-Contrast Parameterization vs Group-Means Parameterization
limma
updated 8 weeks ago by
Gordon Smyth
52k • written 8 weeks ago by
abadgerw
• 0
4
votes
6
replies
1.1k
views
Zero intra-block correlation in RRBS analysis using voomLmFit with block
limma
duplicateCorrelation
edgeR
modelMatrixMeth
voomLmFit
9 weeks ago • updated 8 weeks ago
altintas.ali
• 0
2
votes
2
replies
333
views
Automatically generate contrasts from a model matrix / formula
limma
updated 9 weeks ago by
ATpoint
★ 4.6k • written 9 weeks ago by
Jonathan
▴ 10
3
votes
3
replies
310
views
How to subset voomLmFit (MArrayLM) object?
limma
edgeR
10 weeks ago • updated 9 weeks ago
Guido Hooiveld
★ 4.1k
0
votes
3
replies
357
views
Need help interpreting heatmaps from limma
limma
written 10 weeks ago by
lhwh1208
• 0
2
votes
1
reply
1.4k
views
Removing Effect of Covariate in Only a Subset of Samples in a Model Containing All Samples Loses Full Rank if Factor but not Numerical
limma
updated 10 weeks ago by
James W. MacDonald
67k • written 10 weeks ago by
danphillips28
▴ 10
5
votes
4
replies
470
views
How to control batch effect if Group and batch are same?
limma
DESeq2
BatchEffect
updated 10 weeks ago by
ATpoint
★ 4.6k • written 11 weeks ago by
Bioinformagician
• 0
1
vote
2
replies
475
views
Poor dispersion fit in DESeq2
limma
DESeq2
RNASeq
edgeR
updated 12 weeks ago by
Michael Love
43k • written 3 months ago by
Ben
▴ 10
0
votes
0
replies
233
views
Assistance with Bulk RNA-seq Data Analysis Including Random Effect (`duplicateCorrelation` from `limma`)
limma
RNAseq123
12 weeks ago
tim.meese
• 0
0
votes
2
replies
375
views
EdgeR - Apparent Wrong Direction of logFC with Low Count Data
limma
edgeR
updated 11 weeks ago by
Gordon Smyth
52k • written 12 weeks ago by
blmurphy
• 0
0
votes
2
replies
466
views
Question on performing ANOVA-like analysis using limma
limma
updated 3 months ago by
Gordon Smyth
52k • written 3 months ago by
grubble
• 0
17
votes
9
replies
7.1k
views
How to use removeBatchEffect for removing effect of multiple confounding variables
limma
removebatcheffect
deseq2
updated 3 months ago by
James W. MacDonald
67k • written 8.6 years ago by
ompandey
▴ 10
2
votes
1
reply
360
views
Question: How to Set Up Contrasts for Case-Control Analysis in Limma While Adjusting for Covariates?
MethylationArray
EpigeneticsWorkflow
limma
Regression
updated 3 months ago by
James W. MacDonald
67k • written 3 months ago by
rae.alshammari
▴ 10
0
votes
1
reply
619
views
Unable to install/use limma
limma
Bioconductor
updated 3 months ago by
James W. MacDonald
67k • written 3 months ago by
Jennifer
• 0
3
votes
3
replies
532
views
voom does not seem to properly correct for library size
limma
updated 3 months ago by
Gordon Smyth
52k • written 3 months ago by
ATpoint
★ 4.6k
1
vote
5
replies
840
views
RNASeq normlization for samples with global shift in gene expression
qsmooth
DESeq2
limma
Normalization
updated 3 months ago by
Gordon Smyth
52k • written 4 months ago by
Xuan Yi
• 0
0
votes
3
replies
556
views
Differential expression analysis in time course experiments with DESeq2
DESeq2
limma
DifferentialExpression
updated 4 months ago by
Gordon Smyth
52k • written 4 months ago by
Jay
• 0
3
votes
4
replies
613
views
using limma trend workflow in CAMERA
CAMERA
limma
4 months ago • updated 3 months ago
Jay
• 0
3
votes
3
replies
570
views
One-sided tests with CAMERA-PR
CAMERA
limma
updated 4 months ago by
Gordon Smyth
52k • written 4 months ago by
Tyler Sagendorf
▴ 10
1
vote
6
replies
711
views
limma and Syntactically Invalid Factor Values
limma
updated 5 months ago by
Gordon Smyth
52k • written 5 months ago by
Dario Strbenac
★ 1.5k
1
vote
1
reply
584
views
DEG Analysis using Limma on DSP GeoMx Data: Handling Multiple ROIs per Core
limma
DSP
GeomxTools
5 months ago
Bioinformagician
• 0
4
votes
3
replies
589
views
Limma comparing gene expression between base line and one treatment
limma
updated 5 months ago by
James W. MacDonald
67k • written 5 months ago by
AL
• 0
0
votes
1
reply
544
views
How to rank DEG for GSEA using DEseq2 result ?
DESeq2
limma
GeneSetEnrichment
updated 5 months ago by
ATpoint
★ 4.6k • written 5 months ago by
picasa1983
• 0
2
votes
5
replies
642
views
Limma - 0 downreg and upreg values
GeoMx
limma
DifferentialExpression
updated 5 months ago by
James W. MacDonald
67k • written 5 months ago by
sop
• 0
4
votes
14
replies
1.7k
views
LIMMA 2x2 Factorial Design with Paired Samples
factorialdesign
limma
pairedsamples
updated 4 months ago by
Gordon Smyth
52k • written 5 months ago by
Michael E
• 0
3
votes
4
replies
833
views
Differences in Limma and Dreamlet random effects from confounded dataset
limma
dreamlet
5 months ago
jcollier
• 0
4
votes
4
replies
1.2k
views
Using both voom and removeBatchEffect, will they double adjust the data or is it the right way to perform this
limma
adjustment
voom
removeBatchEffect
covariates
6 months ago
sukeshinik5
▴ 20
0
votes
1
reply
338
views
Deseq2 design matrix
DESeq2
Designmatrix
limma
updated 6 months ago by
James W. MacDonald
67k • written 6 months ago by
Neha
• 0
50 results • Page
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Comment: Normalizing batch effects using technical replicates for DGE analysis between tw
by
yinhjk602
• 0
https://sprunkiphase4.net/ Sprunki Phase 4 is the latest update in the music rhythm game, offering new beats, characters, and challenges. Y…
Comment: Cross-validation with multiple control subgroups in limma
by
Ali Barry
▴ 40
Thanks so much for taking the time here, the insights are much appreciated. I suspected I'd have to ad hoc compare results if I did repeat …
Answer: Cross-validation with multiple control subgroups in limma
by
Gordon Smyth
52k
limma doesn't do cross-validation or resampling. Like most classical linear modeling procedures in statistics, limma uses a statistical mod…
Comment: Issues with seqlevelsStyle when making custom txdb objects for genomes/annotatio
by
Robert Castelo
★ 3.4k
Hi Hervé, thank you very much for your input into how to properly build and manage TxDb objects. gDNAx has two main inputs, one or more BAM…
Comment: Biostrings: Error writing long reads (>200 kbps) with writeQualityScaledXStringS
by
Aidan
▴ 60
Quick update: this is fixed in a branch awaiting PR to Biostrings (https://github.com/Bioconductor/Biostrings/pull/122). Should be in `deve…
Votes
Comment: Issues with seqlevelsStyle when making custom txdb objects for genomes/annotatio
C: edgeR and lack of counts ID on CPM matrix
A: DESeq2 Following RSEM
A: importing RSEM data into DESeq2
A: Expected counts from RSEM in DESeq2
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